Mesenchymal Stem/Progenitors and Other Endometrial Cell Types From Women With Polycystic Ovary Syndrome (PCOS) Display Inflammatory and Oncogenic Potential

Piltonen, Terhi; Chen, J.; Erikson, David W.; Spitzer, Trimble L; Barragan, Fatima; Rabban, Joseph T.; Huddleston, Heather G.; Irwin, Juan C.; Giudice, Linda C.

doi:10.1210/jc.2013-1923

Cited by 85 publications

(65 citation statements)

References 52 publications

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“…Previous studies from our laboratory demonstrate gene expression differences in eMSC and eSF with the presence of pelvic inflammatory disease, namely polysystic ovary syndrome and endometriosis [7,17]. For this reason it is not possible to rule out uterine pathologies as a contributing cause of gene expression differences in the current study.…”

Section: Role Of Uterine Pathologiesmentioning

confidence: 85%

“…Tissue processing for FACS analysis was performed as previously described [4,17]. Briefly, enzymatically dissociated endometrial cells were incubated in blocking buffer (phosphatebuffered saline [PBS] containing 40% human serum and 1% bovine serum albumin [BSA]) for 30 min on ice and then labeled with the following fluorochrome-conjugated antibodies (BD Biosciences) …”

Section: Study Subjects and Tissuesmentioning

confidence: 99%

“…Microarray data analysis was performed using GeneSpring as previously described [4,17]. The intensity values of probe sets in the GeneChip operating software (Affymetrix) were imported into GeneSpring version 11.02 software (Agilent Technologies), normalized and subjected to log 2 transformation using the robust multiarray analysis as the background correction algorithm for ST array technology.…”

Section: Statisticsmentioning

confidence: 99%

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Stromal fibroblasts from perimenopausal endometrium exhibit a different transcriptome than those from the premenopausal endometrium†

Erikson

Barragan

Piltonen

et al. 2017

Biology of Reproduction

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Human endometrium undergoes extensive regeneration on a cyclic basis in premenopausal women and likely occurs through the contribution of stem/progenitor cells. Menopause results in the permanent cessation of menstrual cycles and is preceded by perimenopause, a period of several years in which endocrine and biological changes occur and is a period of risk for endometrial proliferative disorders. The objectives of this study were to identify endometrial mesenchymal stem cells (eMSC) and endometrial stromal fibroblasts (eSF) in endometrium of perimenopausal women and perform expression profile analysis of perimenopausal eMSC and eSF to gain insight into the biology of stem/progenitor and lineage cell populations during the transition to menopause. Endometrial tissue was collected from perimenopausal and premenopausal women (n = 9 each). Microarray analysis was performed on fluorescence-activated cell sorting-isolated eSF and eMSC, and data were validated by quantitative real-time PCR. Principal component analysis showed that cells clustered into three distinct groups in 3-dimensional space: perimenopausal eMSC and premenopausal eMSC clustered together, while perimenopausal eSF and premenopausal eSF formed two discrete clusters separate from eMSC. Hierarchical clustering revealed a branching pattern consistent with principle clustering analysis results, indicating that eMSC from premenopausal and perimenopausal women exhibit similar transcriptomic signatures. Pathway analysis revealed dysregulation of cytoskeleton, proliferation, and survival pathways in perimenopausal vs. Erikson et al., 2017, Vol. 97, No. 3 premenopausal eSF. These data demonstrate that cell populations have altered gene expression in perimenopausal vs. premenopausal endometrium, and that perimenopausal eSF had altered pathway activation when compared to premenopausal eSF. This study provides insight into aging endometrium with relevance to function in reproductively older women. Summary SentenceThe hormonal milieu during the transition to menopause has an effect on endometrial stromal fibroblast gene expression and a minimal effect on the endometrial mesenchymal stem cell population, offering insight into the mechanisms by which the endometrium remains functional after menopause.

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Section: Role Of Uterine Pathologiesmentioning

confidence: 85%

Section: Study Subjects and Tissuesmentioning

confidence: 99%

Section: Statisticsmentioning

confidence: 99%

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Stromal fibroblasts from perimenopausal endometrium exhibit a different transcriptome than those from the premenopausal endometrium†

Erikson

Barragan

Piltonen

et al. 2017

Biology of Reproduction

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“…Активация ERα может быть обусловлена повыше-нием экспрессии ко-активатора стероид-рецептора р160 в эндометрии женщин с СПКЯ в секреторную фазу [14]. В то же время в других относительно не-давних исследованиях не выявлено статистически значимых отличий экспрессии ER у женщин с СПКЯ и в группе контроля с аналогичным индексом массы тела (ИМТ), при этом было показано, что повышенная пролиферация эндометрия специфически коррелиру-ет с ожирением [29]. Последние исследования in vitro продемонстрировали тенденцию к уменьшению экс-прессии рецепторов к прогестерону (PR) с одновре-менным снижением экспрессии инсулиноподобного фактор-связывающего белка 1 (ИФР-СБ1) в ответ на введение эстрадиола [30].…”

Section: маркеры патологии эндометрия у женщин с спкяunclassified

“…Соотношение воспалительных и иммунных клеток изменяется на протяжении менструального цикла, причём фолликулярная фаза характеризуется преобладанием Т-клеток, а секреторная фаза -увели-ченным числом макрофагов и особенно естественных киллеров (клетки uNK) [53]. В недавних работах отмечено, что при СПКЯ в эндометрии пролифера-тивной фазы у женщин повышен воспалительный профиль (высокий уровень ИЛ-6 и цитокина CCL2) и снижено число клеток uNK в поздней секреторной фазе [29].…”

Section: Obstetrics and Gynaecologyunclassified

Endometrial Markers of Polycystic Ovary Syndrome (A Literature Review)

Sharifulin¹,

Шарифулин²,

Сутурина³

2018

Acta Biomedica Scientifica

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Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women and has a significant impact on various aspects of their health and the quality of life. The epidemiology of PCOS is well understood, while the prevalence of this syndrome depends on diagnostic criteria used, the characteristics of the population sample, and vary from 6-10 % to 15 % and higher. A number of studies suggest that the endometrium in women with PCOS differs from the normal endometrium morphologically and functionally. PCOS is associated with infertility problems, higher incidence of pregnancy complications and with increased risk of endometrial cancer, especially when obesity is present.

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Single Cell Transcriptomes Derived from Human Cervical and Uterine Tissue during Pregnancy

Koh

Penland

et al. 2019

Adv. Biosys.

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This work presents the workflow for generating single cell transcriptomes derived from primary human uterine and cervical tissue obtained during planned cesarean hysterectomies. In total, a catalogue of 310 single cell transcriptomes are obtained, cell types present in these biopsies are inferred, and specific genes defining each of the cellular types present in the tissue are identified. Further validation of the inferred cell identity is also demonstrated via meta‐analysis of independent repositories in literature generated by bulk sequenced data of fluorescence‐activated cell sorting sorted cells.

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Mesenchymal Stem/Progenitors and Other Endometrial Cell Types From Women With Polycystic Ovary Syndrome (PCOS) Display Inflammatory and Oncogenic Potential

Cited by 85 publications

References 52 publications

Stromal fibroblasts from perimenopausal endometrium exhibit a different transcriptome than those from the premenopausal endometrium†

Stromal fibroblasts from perimenopausal endometrium exhibit a different transcriptome than those from the premenopausal endometrium†

Endometrial Markers of Polycystic Ovary Syndrome (A Literature Review)

Single Cell Transcriptomes Derived from Human Cervical and Uterine Tissue during Pregnancy

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