Single Cell Transcriptomes Derived from Human Cervical and Uterine Tissue during Pregnancy

Koh, Winston; Wu, Angela Ruohao; Penland, Lolita; Treutlein, Barbara; Neff, Norma; Mantalas, Gary L.; Blumenfeld, Yair J.; El‐Sayed, Yasser Y.; Stevenson, David K.; Shaw, Gary M.; Quake, Stephen R.

doi:10.1002/adbi.201800336

Cited by 14 publications

(11 citation statements)

References 44 publications

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“…The uterus and decidua of control mice also included modest populations of innate immune cells such as Monocyte and macrophage subsets as well as lymphocytes such as T cell, NK cell-1, NK cell-2, and B cell (Figure S1A), likely representing the resident immune populations that have been characterized in human and murine tissues (Bartmann et al, 2014;Li et al, 2018;Pique-Regi et al, 2022;Pique-Regi et al, 2019;Trundley and Moffett, 2004;Vento-Tormo et al, 2018). By contrast with the uterus and decidua, the cervix of control mice comprised a diverse compartment of epithelial subsets (clusters 5, 7, 8, 10, 9 11, 14) and other major cell types (Figure S1A), as previously shown (Chumduri et al, 2021;Koh et al, 2019;Zhao et al, 2021). Immune cells were scarce in the cervix, although a modest Macrophage-1 population was observed (Figure S1A) that is consistent with prior reports of cervical cell composition in late gestation (Dobyns et al, 2015;Dubicke et al, 2016;Osman et al, 2003;Timmons et al, 2009).…”

Section: A Single-cell Atlas Of the Murine Reproductive Tissues Durin...supporting

confidence: 73%

The single-cell atlas of the murine reproductive tissues during preterm labor

Garcia‐Flores

Romero

Peyvandipour

et al. 2022

Preprint

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Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the multi-etiological syndrome of preterm labor. Intra-amniotic infection is the best-established causal link to preterm labor and birth, and involves the premature activation of the parturition cascade in the reproductive tissues (uterus, decidua, and cervix). However, the simultaneous investigation of the cellular landscape and interaction networks in such tissues at single-cell resolution had not been undertaken. Herein, we utilized scRNA-seq to reveal that preterm labor affects the cellularity of the uterus, decidua, and cervix through immune cell infiltration and altered transcriptomic profiles of non-immune cell types. Preterm labor-affected cells showed shared and distinct immune processes, the latter indicating tissue-specific cellular functions. Cell-cell communication analysis implicated immune and non-immune cell types in preterm labor-associated inflammatory pathways across tissues. Next, correlation between uterine transcriptomic data from mice with local or systemic inflammation-induced preterm labor indicated significant overlap in labor-associated gene expression. Last, comparison of cell-cell communication in the murine and human uterus revealed shared signaling pathways implicated in preterm and term parturition. Together, these findings provide novel insight into the cellular landscape, dynamics, and communications in the reproductive tissues during preterm labor leading to premature birth.

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Section: A Single-cell Atlas Of the Murine Reproductive Tissues Durin...supporting

confidence: 73%

The single-cell atlas of the murine reproductive tissues during preterm labor

Garcia‐Flores

Romero

Peyvandipour

et al. 2022

Preprint

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show abstract

“…With advances in technology and development analysis pipelines, studies have reached the single-cell level 22 – 25 . To the best of our knowledge, all cell populations throughout the menstrual cycle were included in our study, which provided the most detailed and dynamic cell populations of the uterine tissue to date in comparison with previous bulk, single-cell studies on endometrial tissue or in vitro endometrial organoids 15 , 24 , 26 , 27 .…”

Section: Discussionmentioning

confidence: 99%

SFRP4+ stromal cell subpopulation with IGF1 signaling in human endometrial regeneration

Nie

et al. 2022

Cell Discov

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Our understanding of full-thickness endometrial regeneration after injury is limited by an incomplete molecular characterization of the cell populations responsible for the organ functions. To help fill this knowledge gap, we characterized 10,551 cells of full-thickness normal human uterine from two menstrual phases (proliferative and secretory phase) using unbiased single cell RNA-sequencing. We dissected cell heterogeneity of main cell types (epithelial, stromal, endothelial, and immune cells) of the full thickness uterine tissues, cell population architectures of human uterus cells across the menstrual cycle. We identified an SFRP4+ stromal cell subpopulation that was highly enriched in the regenerative stage of the human endometria during the menstrual cycle, and the SFRP4+ stromal cells could significantly enhance the proliferation of human endometrial epithelial organoid in vitro, and promote the regeneration of endometrial epithelial glands and full-thickness endometrial injury through IGF1 signaling pathway in vivo. Our cell atlas of full-thickness uterine tissues revealed the cellular heterogeneities, cell population architectures, and their cell–cell communications during the monthly regeneration of the human endometria, which provide insight into the biology of human endometrial regeneration and the development of regenerative medicine treatments against endometrial damage and intrauterine adhesion.

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“… 53 , 69 Proteomic studies of cervical mucus from NP rhesus macaque 70 and proteomic or limited single-cell analysis of cervical samples from pregnant women highlight species conservation of noted factors observed in the goblet 1 cluster. 12 , 71 , 72 , 73 , 74 …”

Section: Discussionmentioning

confidence: 99%