Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow

Abstract

We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.

Use of bone mesenchymal stem cells to treat rats with acute liver failure

“…BMSC therapy currently represents a promising strategy for ALF treatment (Sato et al, 2005;Cho et al, 2009). The present study demonstrated that BMSC transplantation attenuates liver damage by reducing the infiltration of inflammatory cells and inhibiting apoptosis.…”

“…Cell suspensions were incubated with different antibodies, including FITC-labeled anti-rat CD45, CD29, CD11, and CD90 (Sato et al, 2005;Cho et al, 2009) (all from BioLegend, USA), at a recommended dilution (1:100) for 30 min at room temperature in the dark. Isotype-identical antibodies (IgG) served as controls.…”

“…Group B contained the portal vein group, in which rats were anesthetized and subjected to an upper midline incision inferiorly from the xiphoid, and the abdominal cavity was exposed with a retractor. The portal vein was gently isolated and directly injected with 1.4 x 10 7 cells/kg BMSCs using a 25-gauge needle connected to a 2-mL syringe (Sato et al, 2005;Cho et al, 2009). The abdominal wall was closed after bleeding was stopped by pressure.…”

Escenario actual y perspectivas de la terapia con células madre mesenquimales en medicina intensiva

“…Son multipotenciales, diferencián-dose hacia adipocitos, osteoblastos, condrocitos, tenocitos, miocitos, estroma hematopoyético y fibroblastos 6 . Presentan plasticidad para diferenciarse ex-vivo a tejidos no mesodermales, como neuronas, astrocitos, oligodendrocitos, neumocitos y hepatocitos 7,8 . La Sociedad Internacional de Terapia Celular (ISCT) ha sugerido criterios mínimos para definir una MSC: a) adherencia al plástico bajo condiciones estándar de cultivo; b) expresión de marcadores de superficie como CD105, CD90, CD73 y ausencia de marcadores, sobre todo hematopoyéticos, incluyendo CD45, CD34, CD14 y CD11b; c) capacidad de diferenciación in-vitro hacia los otros linajes de origen mesodermal (adipocitos, condrocitos, osteoblastos) 9 .…”

Liver Stem Cells Derived from the Bone Marrow and Umbilical Cord Blood

“…Biological signals released from the injured area and corresponding receptors expressed on the cell surface of MSCs are critical determinants of this step (16).…”