Liver Stem Cells Derived from the Bone Marrow and Umbilical Cord Blood

Ryu, Kyung Ha

doi:10.15283/ijsc.2009.2.2.97

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Coculture of rat hepatocytes with pancreatic islets (41) and rat hepatocytes with NIH/3T3 fibroblasts (42,43) enhanced spheroid formation and functions of hepatocytes.…”

Section: Coculture Systemmentioning

confidence: 99%

Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering

Jiang¹,

Kim²,

Cho³

et al. 2010

Int J Stem Cells

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The liver plays an important role in a broad spectrum of physiological functions and detoxifies endogenous and exogenous substances. The liver failure is associated with a high risk of mortality because it is one of important organs in our body. Various bioartificial liver (BAL) systems have been used for clinical trials as a bridge for liver transplantations in patients with liver failure. Long term and stable liver-specific functions of hepatocytes in the development of BAL support systems should be considered. Spheroid formation of hepatocytes enhances liver-specific functions. In this review, hepatocyte spheroid formation methods such as galactose density, topology of extracellular matrix, micromolding technique, hanging-drop culture, non-adhesive surface, positive charged surface, spinner culture, rocked technique, medium component, external forces, coculture system and polymeric nanospheres are explained for enhancing liver-specific functions.

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“…Coculture of rat hepatocytes with pancreatic islets (41) and rat hepatocytes with NIH/3T3 fibroblasts (42,43) enhanced spheroid formation and functions of hepatocytes.…”

Section: Coculture Systemmentioning

confidence: 99%

Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering

Jiang¹,

Kim²,

Cho³

et al. 2010

Int J Stem Cells

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“…A stimulus to regeneration such as two-thirds partial hepatectomy promotes increases in carbamyl phosphate synthetase I activity with subsequent liver hypertrophy. This early experience suggests that this therapeutic approach has the potential of enhancing and accelerating hepatic regeneration in a clinical setting (24). Much of the excitement in stem cell research centers on embryonic stem cells because of their high plasticity, but this approach remains controversial for ethical reasons (25).…”

Section: Intrasplenic Injectionmentioning

confidence: 99%

Migration of Human Umbilical Cord Blood Cells into Rat Liver

et al. 2010

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Background and Objectives: Cell therapy provides an effective strategy for the treatment of an impaired liver. Human umbilical cord blood progenitor cells have the potential to differentiate into hepatocytes. Progenitor cells transplanted into the spleen could migrate directly into the liver through portal circulation. To track migration of human umbilical cord blood progenitor cells in cirrhotic rat liver after intrasplenic transplantation and to prove the possibility similar behavior of human umbilical cord blood nucleated cells in humans. Methods and Results: Umbilical cord blood samples from full-term deliveries will be collected after obtaining an informed consent from the mother. The collection procedure will be conducted after completion of delivery and will not interfere with the normal obstetric procedures. Adult male Sprague Dawley rats were subjected to liver cirrhosis by intraperitoneal injection of thioacetamide. Cirrhotic rats were treated with human umbilical cord blood nucleated cells by intra-splenic transplantation. Migration of intrasplenic transplanted human umbilical cord blood cells to the liver was successfully documented with Immunohistochemistry. The liver and spleen from recipient animals were removed. Histopathological and immunohistochemical analysis were performed 20 weeks after intrasplenic injection of the cells. Intrasplenically injected cells migrate to the liver of recipient animals. Conclusions: Human cord blood nucleated cells have the potential to differentiate into hepatocytes and substantially improve the histology and function of the cirrhotic liver in rats. Relocation into liver after intrasplenic transplantation could be detected by immunohistochemistry. Transdifferentiated cells could be efficiently stained with antihuman hepatocytes.

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