Mesenchymal stem cells in the treatment of spinal cord injuries: A review

Dasari, Venkata Ramesh; Veeravalli, Krishna Kumar; Dinh, Dzung H.

doi:10.4252/wjsc.v6.i2.120

Cited by 177 publications

(144 citation statements)

References 82 publications

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…Therefore, rAT-MSCs and rBM-MSCs have the potential to support the regeneration process directly or indirectly. A number of studies on different types of animal models and on human subjects have demonstrated functional recovery by MSCs (1, 6,10,12,18,19,27,29).…”

Section: █ Introductionmentioning

confidence: 99%

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

Aras¹,

Sabancı²,

Kabataş³

et al. 2015

Turkish Neurosurgery

View full text Add to dashboard Cite

AIM:To investigate the effectiveness of rat adipose tissue-derived (rAT) mesenchymal stem cell (MSC) transplantation on the functional restoration and regeneration of spinal cord injury (SCI). MATERIAl and METhODS:Six of 48 Wistar albino rats were sacrificed to obtain MSCs, and the remaining rats were divided randomly into six groups. SCI was performed using the clip compression method. The control and transplantation groups were injected with physiological saline and a rAT-MSC suspension at the injury sites, respectively. Each animal was evaluated using the Basso, Beattie and Bresnahan (BBB) rating system and sacrificed at 28 days post-injury period (p.i.). The regeneration process was evaluated based on immunostaining against β3-tubulin, BDNF, CNTF, and CNPase.RESulTS: rAT-MSC transplantation into the SCI site substantially improved the tissue regeneration and functional recovery (p < 0.05). However, the rAT-MSC transplantation at 9 days p.i. was not more efficient on functional recovery than the transplantation immediately after injury. The expression of β3-tubulin, BDNF and CNTF at the injury site indicated the potential for functional regeneration. COnCluSIOn:The adaptive nature of rat-MSCs enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of SCI.

show abstract

Section: █ Introductionmentioning

confidence: 99%

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

Aras¹,

Sabancı²,

Kabataş³

et al. 2015

Turkish Neurosurgery

View full text Add to dashboard Cite

show abstract

“…Another approach for enhancing the expansion of OECs is their reversible immortalization [Lim et al, 2010], and/or the application of induced pluripotent stem cells [Lee-Kubli and Lu, 2015], or even mesenchymal stem cells [Dasari et al, 2014]. We also noticed the smallest percentage of senescence cells and the highest percentage of living cells in the culture of the first group of donors when compared to the second group.…”

Section: Discussionmentioning

confidence: 51%

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Marycz

Kornicka²,

Grzesiak³

et al. 2017

Cells Tissues Organs

View full text Add to dashboard Cite

The olfactory bulb (OB) is a unique structure in the central nervous system that retains the ability to create new neuronal connections. Glial cells isolated from the OB have been recently considered as a novel and promising tool to establish an effective therapy for central nervous system injuries. Due to the hindered access to autologous tissue for cell isolation, an allogeneic source of tissues obtained postmortem has been proposed. In this study, we focused on the morphological and molecular characteristics of human OB-derived glial cells isolated postmortem, at different time points after a donor's death. We evaluated the proliferative activity of the isolated cells, and investigated the ultrastructure of the mitochondria, the accumulation of intracellular reactive oxygen species, and the activity of superoxide dismutase. The data obtained clearly indicate that the duration of ischemia is crucial for the viability/senescence rate of OB-derived glial cells. The OB can be isolated during autopsy and still stand as a source of viable glial cells, but ischemia duration is a major factor limiting its potential usefulness in therapies.

show abstract

“…MSCs have been shown to differentiate into neural cells and astrocytes both in vitro and in vivo, exhibiting the ability to differentiate into astrocyte-like cells when transplanted into the normal or ischemic brain (24). However, despite these apparent abilities, the prevailing belief regarding the neuroprotective effects of MSCs is that of paracrine factors, which are secreted by these cells in response to damage (4,5,7). MSCs are known to secrete several growth factors, including brain-derived neurotrophic factor, vascular endothelial growth factor, neural growth factor, glia cell line-derived neurotrophic factor, and insulin-like growth factor, which play major roles in neuroprotection (5,25).…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of bone marrow-derived mesenchymal stem cellsand conditioned medium in mechanically injured neuroblastoma cells

et al. 2016

View full text Add to dashboard Cite

IntroductionStem cells exhibit self-renewal characteristics that distinguish them from other cell types. Major subtypes include totipotent cells, which can divide to produce all cells in an organism, pluripotent cells, which are able to differentiate into any of the three germ layers (endoderm, ectoderm, or mesoderm), and multipotent cells, which can produce multiple cell types within a given lineage (1). Mesenchymal stem cells (MSCs) constitute a class of multipotent cells capable of differentiating into many diverse cell types, including neural cells, chondroblasts, adipocytes, bone, skeletal muscle, and connective tissue (2-4). These cells can be isolated from almost any tissue, including bone marrow, Wharton jelly, umbilical cord, placenta, dental pulp, and adipose tissue using a simple procedure (5). Identification of MSCs is primarily performed using specific phenotypic and cell surface markers. MSCs should be able to adhere to a plastic surface and express defined surface antigens such as CD29, CD73, CD90, and CD105; however, they should also be negative Background/aim: Bone marrow-derived mesenchymal stem cells (BMSCs) possess self-renewal characteristics that distinguish them from other cell types. Recent studies have focused on the effects of conditioned medium (CM) that includes the extracellular matrix.Here we examined the neuroprotective effects of BMSCs and CM on damaged neuroblastoma cells. Materials and methods:The cells were divided into five groups: 1) healthy controls, 2) damaged cells alone, 3) damaged cells treated with BMSCs, 4) damaged cells treated with CM, and 5) damaged cells treated with both BMSCs and CM. Neuroprotective effects were then evaluated based upon the levels of oxidative stress, antitransforming growth factor β1 (anti-TGFβ1) production, and apoptosis.Results: Significant differences were observed between healthy controls and damaged cells (P < 0.001), as well as between damaged cells and those treated with BMSCs alone (P < 0.05), CM alone (P < 0.05), and both BMSCs and CM in combination (P < 0.01). Among the treated groups, the strongest neuroprotective effects were seen in cells treated with both BMSCs and CM. Conclusion:These results show that both BMSCs and CM exhibit neuroprotective effects in damaged neuroblastoma cells. The strongest benefits were seen following treatment with both BMSCs and CM.

show abstract

Mesenchymal stem cells in the treatment of spinal cord injuries: A review

Cited by 177 publications

References 82 publications

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Neuroprotective effects of bone marrow-derived mesenchymal stem cellsand conditioned medium in mechanically injured neuroblastoma cells

Contact Info

Product

Resources

About