Mesenchymal stem cells from human adipose tissue and bone repair: a literature review

Alonso-Goulart, Vivian; Ferreira, Luzitano Brandão; Duarte, Cristiane Angélico; Lima, Isabela Lemos de; Ferreira, Enza Rafaela; Oliveira, Bárbara Cândido de; Vargas, Luna Nascimento; Moraes, Dayane Dotto de; Silva, Isaura Beatriz Borges; Faria, Rafael de Oliveira; Souza, Aline Gomes de; Castro-Filice, Letícia de Souza

doi:10.1016/j.biori.2017.10.005

Cited by 43 publications

(41 citation statements)

References 49 publications

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“…Hematopoietic stem cells, neural stem cells, and mesenchymal stem cells are examples of adult stem cells (4,5). For many years, the bone marrow has been the most important source of mesenchymal stem cells, but recently adipose tissue has gained a prominent place as the source of mesenchymal stem cells because these cells are more comfortable to separate from the adipose tissue under the skin and have fewer side effects (6).…”

Section: R E T Rmentioning

confidence: 99%

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

et al. 2020

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Background: Tissue engineering is the science of tissue design and one of the main branches of regenerative medicine that aims to improve and repair tissue injuries. Objectives: This study aimed to decellularize the tissue of bovine Achilles tendon and create a natural 3D scaffold. Then, the interaction of human adipose tissue-derived mesenchymal stem cells (hAd-MSCs) with this 3D scaffold was evaluated for use in tendon injuries. Methods: The bovine Achilles tendon was obtained from a slaughterhouse and decellularized by the combination of ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS). Histological and biomechanical tests were used to evaluate the quality of decellularized scaffolds. Adipose-derived mesenchymal stem cells were cultured on scaffolds, and cell viability and cell behavior were evaluated by the MTT test and scanning electron microscopy. Results: The results of histological and biomechanical tests showed the complete removal of cells with the preservation of the extracellular matrix. The results of cell culture on scaffolds also showed that optical absorption in scaffolds containing cells increased over time. Conclusions: In general, the decellularized scaffold in this study did not undergo significant structural changes in the tendon tissue. The interaction between hAd-MSCs and the decellularized scaffold revealed that the scaffold was somehow suitable for cell culture. However, it needs to be more investigated for use in the treatment of tendon injuries of the athletes.

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Section: R E T Rmentioning

confidence: 99%

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

et al. 2020

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“…Another important issue to prevent unwanted clinical complications during implantation is maintaining a permanent connection between the connective tissue and the implant [1,11,30,40,41,42,43,44]. For this reason, we used the co-culture system of ADSCs with osteoblasts and fibroblasts in the present study to verify the efficacy of the engineered bone tissue integration.…”

Section: Introductionmentioning

confidence: 99%

Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies

Ehlert

Roszek

Jędrzejewski

et al. 2019

IJMS

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The increasing need for novel bone replacement materials has been driving numerous studies on modifying their surface to stimulate osteogenic cells expansion and to accelerate bone tissue regeneration. The goal of the presented study was to optimize the production of titania-based bioactive materials with high porosity and defined nanostructure, which supports the cell viability and growth. We have chosen to our experiments TiO2 nanofibers, produced by chemical oxidation of Ti6Al4V alloy. Fibrous nanocoatings were characterized structurally (X-ray diffraction (XRD)) and morphologically (scanning electron microscopy (SEM)). The wettability of the coatings and their mechanical properties were also evaluated. We have investigated the direct influence of the modified titanium alloy surfaces on the survival and proliferation of mesenchymal stem cells derived from adipose tissue (ADSCs). In parallel, proliferation of bone tissue cells—human osteoblasts MG-63 and connective tissue cells - mouse fibroblasts L929, as well as cell viability in co-cultures (osteoblasts/ADSCs and fibroblasts/ADSCs has been studied. The results of our experiments proved that among all tested nanofibrous coatings, the amorphous titania-based ones were the most optimal scaffolds for the integration and proliferation of ADSCs, fibroblasts, and osteoblasts. Thus, we postulated these scaffolds to have the osteopromotional potential. However, from the co-culture experiments it can be concluded that ADSCs have the ability to functionalize the initially unfavorable surface, and make it suitable for more specialized and demanding cells.

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“…Notably, recent studies have provided evidence that the number of ADSCs exceeds the frequency of marrow-derived mesenchymal stem cells (BMSC) found in the medullary stroma 60 . Approximately, 1 ml of adipose tissue obtained by liposuction contains about 6 × 10 5 to 2 × 10 6 ADSCs 61 , this represents at least 500 times more cells than the number of stem cells obtained from bone marrow 62 – 64 . Taken together these data highlight adipose tissue as the richest source of multipotent stem cells, possibly outperforming any other source in the body.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer

Castro-Oropeza

Vázquez-Santillán

Díaz-Gastelum

et al. 2020

Sci Rep

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Epidemiological studies indicate that obesity negatively affects the progression and treatment of cervical-uterine cancer. Recent evidence shows that a subpopulation of adipose-derived stem cells can alter cancer properties. In the present project, we described for the first time the impact of adipose-derived stem cells over the malignant behavior of cervical cancer cells. The transcriptome of cancer cells cultured in the presence of stem cells was analyzed using RNA-seq. Changes in gene expression were validated using digital-PCR. Bioinformatics tools were used to identify the main transduction pathways disrupted in cancer cells due to the presence of stem cells. In vitro and in vivo assays were conducted to validate cellular and molecular processes altered in cervical cancer cells owing to stem cells. Our results show that the expression of 95 RNAs was altered in cancer cells as a result of adipose-derived stem cells. Experimental assays indicate that stem cells provoke an increment in migration, invasion, angiogenesis, and tumorigenesis of cancer cells; however, no alterations were found in proliferation. Bioinformatics and experimental analyses demonstrated that the NF-kappa B signaling pathway is enriched in cancer cells due to the influence of adipose-derived stem cells. Interestingly, the tumor cells shift their epithelial to a mesenchymal morphology, which was reflected by the increased expression of specific mesenchymal markers. In addition, stem cells also promote a stemness phenotype in the cervical cancer cells. In conclusion, our results suggest that adipose-derived stem cells induce cervical cancer cells to acquire malignant features where NF-kappa B plays a key role.

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Mesenchymal stem cells from human adipose tissue and bone repair: a literature review

Cited by 43 publications

References 49 publications

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

Preparation of Decellularized Bovine Tendon Scaffold and Evaluation of Its Interaction with Adipose Tissue-Derived Mesenchymal Stem Cells

Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies

Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer

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