Mesenchymal Stem Cells Combined With IFNγ Induce Apoptosis of Breast Cancer Cells Partially Through TRAIL

Yenilmez, Ezgi Nurdan; Genç, Deniz; Farooqı, Ammad Ahmad; Tunoğlu, Servet; Zeybek, Ümit; Akkoç, Tunç; Yaylım, İlhan

doi:10.21873/anticanres.14577

Cited by 12 publications

(5 citation statements)

References 24 publications

(28 reference statements)

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“…Conversely, there are studies indicating that MSCs can attenuate colon cancer growth [33,38]. We assume that while adult-type MSCs such as bone marrow-derived and adipose-derived MSCs induce cancer, also adult-type but 'younger' MSCs such as Wharton's jelly-derived and cord blood-derived MSCs may have a higher capacity to drive cancer cells to apoptosis [39][40][41]. In addition, we are agreed that the dual role in the effects of MSCs may be affected by the method used in the isolation of mesenchymal stem cells, the number of passages used, different level of secreted growth factors and the differences in the culture medium [42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

The in vitro treatment of mesenchymal stem cells for colorectal cancer cells

et al. 2023

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Colorectal cancer is the most common tumor of the gastrointestinal system. The conventional treatment options of colorectal cancer are troublesome for both patients and clinicians. Recently, mesenchymal stem cells (MSCs) have been the novel focus for cell therapy due to its migration to tumor sites. In this study, the apoptotic effect of MSCs on colorectal cancer cell lines has been aimed. were selected as the colorectal cancer cell lines. Human umbilical cord blood and Wharton's jelly were used as mesenchymal stem cell sources. To discriminate against the apoptotic effect of MSC on cancer, we also used peripheral blood mononuclear cells (PBMC) as a healthy control group. Cord blood-MSC and PBMC were obtained by coll-paque density gradient, and Wharton's jelly-MSC by explant method.Transwell co-culture systems were used as cancer cells or PBMC/MSCs at ratios of 1/5 and 1/10, incubation times of 24 hours and 72 hours. The Annexin V/PI-FITC based apoptosis assay was performed by ow cytometry. Caspase-3 and HTRA2/Omi proteins were measured by ELISA. For both ratios in both cancer cells, it was found that the apoptotic effect of Wharton's jelly-MSC was signi cantly higher in 72-hour incubations (p<0.006), whereas the effect of cord blood mesenchymal stem cell in 24hour incubations were higher (p<0.007). In this study, we showed that human cord blood and tissue derived MSCs treatment led colorectal cancers to apoptosis. We anticipate that further in vivo studies may shed light on the apoptotic effect of MSC. *For PBMC and MSCs treatment comparison, Wilcoxon test were used. The signi cance value was determined as p<0.05.Table 2 The comparison of HTRA2/Omi related to change of MSC sources

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Section: Discussionmentioning

confidence: 99%

The in vitro treatment of mesenchymal stem cells for colorectal cancer cells

et al. 2023

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“…Apoptosis, a method of programmed cell death, is often blocked in cancer cells. Human Wharton's jelly mesenchymal stem cell secretomes have the ability to induce selective death of cancer cells and spare healthy cells, as evidenced by its ability to accelerate apoptosis of breast cancer cells (23).…”

Section: Discussionmentioning

confidence: 99%

Suppressing Effect of Human Wharton’s Jelly Mesenchymal Stem Cell Secretomes on Oxidative Stress Induced by Breast Cancer Cell Line SK-BR3

Ahmadpour,

Karimi,

Saadatmandfar

et al. 2023

Jentashapir J Cell Mol Biol

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Background: The main reason for treatment failure and the primary cause of breast cancer deaths is metastasis. Cancer features, such as epithelial to mesenchymal transition (EMT), invasiveness, stemness, and ability to metastasize, are significantly influenced by oxidative stress. Objectives: The primary objective of this work was to evaluate the effects of human Wharton’s jelly mesenchymal stem cell secretomes (hWJMSCs-Se) on oxidant contents and development of the breast cancer SK-BR3 cell line and alterations in EMT markers genes after treatment. Methods: SK-BR3 cells received 48 hours of treatment with 10, 25, or 50 μg/mL hWJMSCs-Se. The MTT test and colony formation were used to evaluate the SK-BR3 cells’ viability and proliferation capability. By using annexin V/propidium iodide (PI) staining, apoptosis was determined. The messenger ribonucleic acid (mRNA) expression levels in genes associated with antioxidants were additionally assessed. Antioxidant enzyme activity was checked after SK-BR3 treatment with hWJMSCs-Se. Results: In the hWJMSCs-Se-treated SK-BR3 cells, colony counts, and viability percentages decreased significantly with time and concentration. The treated cells displayed considerably greater apoptotic indices when compared to the control. Catalase (CAT), superoxide dismutase (SOD) activities, and glutathione (GSH) content were significantly greater in the hWJMSCs-Se-exposed SK-BR3 cells. The Vimentin gene and N-cadherin gene were significantly elevated in the treated cells, and E-cadherin and β-catenin decreased conversely. Conclusions: The present study suggests that the use of hWJMSCs in the treatment of human epidermal growth factor receptor 2 (HER2)-positive malignancies provides an innovative solution for cancer therapy. As the oxidant level and EMT pathway decreased, breast cancer cell growth was significantly restricted, and mortality increased.

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“…TRAIL-resistant cancer cells have hyperactive survival signaling. Different natural and synthetic molecules have been tested to increase the sensitivity of cancer cells to TRAIL-based therapeutics (80)(81)(82)(83)(84).…”

Section: Trail-based Therapeutics Mediated Pyroptosismentioning

confidence: 99%

Mechanistic role of pyroptosis in tumor microenvironment and tumor immunotherapy

Rukset Attar,

Muhammad Zahid Qureshi,

Uteuliyev Yerzhan Sabitaliyevich

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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In recent decades, extraordinary attention has been devoted to cell death pathways principally because of multifaceted regulatory roles in normal developmental and pathophysiological processes. The removal of functionally defective, infected or potentially malignant cells is regulated by programmed cell death (PCD) cascades. Pyroptotic cell death is a highly complicated pro-inflammatory form of cell death. Pyroptosis is characterized by the formation of pores in the plasma membrane by oligomerization of the N-terminal fragment of gasdermins (gasdermin-NT) following the cleavage of gasdermin. Pyroptosis plays a pivotal role in the innate immune responses and mechanistically steered by inflammasome-mediated and inflammasome-independent cascades. In this review, we have comprehensively analyzed how different signaling pathways regulated pyroptosis in cancer inhibition and metastatic spread of cancer cells to the secondary sites. Comprehensive understanding of the interconnection between signaling pathways and pyroptosis will enable us to reap maximum benefits from the exciting mechanistic insights gained from pioneering studies related to pyroptosis.

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Mesenchymal Stem Cells Combined With IFNγ Induce Apoptosis of Breast Cancer Cells Partially Through TRAIL

Cited by 12 publications

References 24 publications

The in vitro treatment of mesenchymal stem cells for colorectal cancer cells

The in vitro treatment of mesenchymal stem cells for colorectal cancer cells

Suppressing Effect of Human Wharton’s Jelly Mesenchymal Stem Cell Secretomes on Oxidative Stress Induced by Breast Cancer Cell Line SK-BR3

Mechanistic role of pyroptosis in tumor microenvironment and tumor immunotherapy

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