Mesenchymal Stem Cells Augment the Adaptive Response to Eccentric Exercise

Zou, Kai; Huntsman, Heather D.; Valero, M. Carmen; Adams, Joseph T.; Skelton, Jack T.; Lisio, Michael De; Jensen, Tor; Boppart, Marni D.

doi:10.1249/mss.0000000000000405

Cited by 23 publications

(29 citation statements)

References 29 publications

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“…To quantify myofiber CSA, dystrophin-Alexa Fluor 633 images were imported into Adobe Photoshop (CS5 Extended; Adobe, San Jose, CA, USA), and an average of 400 fibers were circled using the magnetic lasso tool, which decreases subjectivity and interassessment error (26). To determine MHC myofiber CSA, ;100-150 myofibers per type were circled on average as described above.…”

Section: Immunofluorescence Image Analysismentioning

confidence: 99%

“…Overall, these findings suggest that manipulation of SCs may not be the optimal approach to improve recovery postdisuse. Alternative stem and stromal cells, including mesenchymal stem/stromal cells and pericytes, exist in skeletal muscle that similarly demonstrate capacity for repair following injury, yet the ability of these cells to induce growth and recover mass following disuse remains unexplored (23)(24)(25)(26).…”

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confidence: 99%

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Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization

et al. 2019

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Conditions of extended bed rest and limb immobilization can initiate rapid and significant loss of skeletal muscle mass and function. Physical rehabilitation is standard practice following a period of disuse, yet mobility may be severely compromised, and recovery is commonly delayed or incomplete in special populations. Thus, a novel approach toward recovery of muscle mass is highly desired. Pericytes [neuron‐glial antigen 2 (NG2)+CD31−CD45−(Lineage− [Lin−]) and CD146+Lin−] demonstrate capacity to facilitate muscle repair, yet the ability to enhance myofiber growth following disuse is unknown. In the current study, 3–4‐mo‐old mice were unilaterally immobilized for 14 d (IM) or immobilized for 14 d followed by 14 d of remobilization (RE). Flow cytometry and targeted gene expression analyses were completed to assess pericyte quantity and function following IM and RE. In addition, a transplantation study was conducted to assess the impact of pericytes on recovery. Results from targeted analyses suggest minimal impact of disuse on pericyte gene expression, yet NG2+Lin− pericyte quantity is reduced following IM (P < 0.05). Remarkably, pericyte transplantation recovered losses in myofiber cross‐sectional area and the capillary‐to‐fiber ratio following RE, whereas deficits remained with vehicle alone (P = 0.01). These findings provide the first evidence that pericytes effectively rehabilitate skeletal muscle mass following disuse atrophy.—Munroe, M., Dvoretskiy, S., Lopez, A., Leong, J., Dyle, M. C., Kong, H., Adams, C. M., Boppart, M. D. Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization. FASEB J. 33, 7694–7706 (2019). http://www.fasebj.org

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Section: Immunofluorescence Image Analysismentioning

confidence: 99%

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confidence: 99%

Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization

et al. 2019

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“…In particular, although limited, it has been demonstrated on the following cells: human skeletal muscle pericytes [67, 68] and human Mesenchymal Sca1 + CD45 − cells[69]. …”

Section: Non-satellite Cellsmentioning

confidence: 99%

Muscle stem cell and physical activity: what point is the debate at?

et al. 2017

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AbstractIn the last 15 years, it emerged that the practice of regular physical activity reduces the risks of many diseases (cardiovascular diseases, diabetes, etc.) and it is fundamental in weight control and energy consuming to contrast obesity. Different groups proposed many molecular mechanisms as responsible for the positive effects of physical activity in healthy life. However, many points remain to be clarified. In this mini-review we reported the latest observations on the effects of physical exercise on healthy skeletal and cardiac muscle focusing on muscle stem cells. The last ones represent the fundamental elements for muscle regeneration post injury, but also for healthy muscle homeostasis.Interestingly, in both muscle tissues the morphological consequence of physical activity is a physiological hypertrophy that depends on different phenomena both in differentiated cells and stem cells. The signaling pathways for physical exercise effects present common elements in skeletal and cardiac muscle, like activation of specific transcription factors, proliferative pathways, and cytokines. More recently, post translational (miRNAs) or epigenetic (DNA methylation) modifications have been demonstrated. However, several points remain unresolved thus requiring new research on the effect of exercise on muscle stem cells.

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“…137,146 Interestingly, transplantation of young (5 week) musclederived mMSCs (1 Â 10 4 ) into recipient muscle 1 h following an acute bout of exercise (protocol described above) significantly increased satellite cell number and new fiber synthesis 141 In addition, transplantation increased myonuclear accumulation, myofiber growth, and strength following repeated bouts of eccentric exercise (3Â/week, 4 weeks) in adult mice. 147 In both studies, mMSCs did not express Pax7, form new fibers, or fuse with existing fibers. The fact that transplanted cells localized to areas of CLN + fibers and vessels and contributed to vessel remodeling following an acute bout of exercise 148 suggests the ability of mMSCs to repair multiple tissues via release of local factors.…”

Section: Mesenchymal-like Stem Cells In Skeletal Muscle and Theirmentioning

confidence: 88%