Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation

Tsai, Yu-Chen; Cheng, Tai-Shan; Liao, Hua‐Fang; Chuang, Ming‐Hsi; Chen, Huiting; Chen, Chun‐Hung; Zhang, Kai-Ling; Chang, Chih-Hung; Lin, Po Ting; Huang, Chi Ying F.

doi:10.1007/s13770-022-00490-0

Cited by 7 publications

(6 citation statements)

References 64 publications

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“…A stem cell spheroid culture was employed in this investigation. Stem cell approaches have attracted a lot of attention, and stem cells have been applied for regenerative medicine [27][28][29][30][31][32][33][34]. By enabling cell-cell and cell-matrix interactions, a spheroid culture technique replicated the physicochemical environment in vivo while overcoming the drawbacks of a conventional monolayer cell culture [35].…”

Section: Discussionmentioning

confidence: 99%

Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells

Kim

Hwa

et al. 2023

Medicina

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Background and Objectives: Vitamin E is reported to expedite new bone formation in animal models, and this has led to a decrease in the time needed for treatment. In this study, human gingiva-derived stem cell-derived spheroids were examined to determine the effects of vitamin E on cell survival, osteogenic differentiation, and mineralization. Materials and Methods: Human gingiva-derived stem cells were used to create spheroids, which were then cultivated with vitamin E at doses of 0, 0.1, 1, 10, and 100 ng/mL. The morphological examination and the qualitative and quantitative vitality of the cells were assessed. Alizarin Red S staining and alkaline phosphatase activity assays were performed on days 7 and 14 to evaluate the osteogenic differentiation. The expression levels of RUNX2 and COL1A1 were assessed using a real-time polymerase chain reaction. Results: The addition of vitamin E did not appear to alter the spheroid’s shape at the measured quantities without altering the diameter. During the culture time, the majority of the cells in the spheroids fluoresced green. Regardless of concentration, there were substantial increases in cell viability in the vitamin E-loaded groups on day 7 (p < 0.05). On day 14, the Alizarin Red S staining was statistically higher in the 1 ng/mL group compared to the unloaded control (p < 0.05). The addition of vitamin E to the culture enhanced the mRNA expression levels of RUNX2, OCN, and COL1A1 based on the real-time polymerase chain reaction data. Conclusions: We draw the conclusion that vitamin E may be used to promote the osteogenic differentiation of stem cell spheroids in light of these data.

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Section: Discussionmentioning

confidence: 99%

Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells

Kim

Hwa

et al. 2023

Medicina

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“…In this section, we will discuss the regenerative repair capabilities of BMSC-EVs in orthopedic diseases. MSC-EVs improve the bone microenvironment and inhibit bone metastasis, representing a promising novel therapeutic for the prevention of bone and joint diseases and disease rehabilitation, with potential as a diagnostic and therapeutic tool for aging-related degenerative diseases [74,75] . MSC-derived EVs have successfully been used as cell-free therapies to guide cartilage differentiation in adipose-derived stem cells [76] .…”

Section: Osteoarthritismentioning

confidence: 99%

Mesenchymal stem cell-derived extracellular vesicles for human diseases

Zhang,

Che,

Zhang

et al. 2024

Extracell Vesicles Circ Nucleic Acids

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Stem cell therapy is a novel approach for treating various severe and intractable diseases, including autoimmune disorders, organ transplants, tumors, and neurodegenerative diseases. Nevertheless, the extensive utilization of stem cells is constrained by potential tumorigenicity, challenges in precise differentiation, rejection concerns, and ethical considerations. Extracellular vesicles possess the ability to carry diverse bioactive factors from stem cells and deliver them to specific target cells or tissues. Moreover, they offer the advantage of low immunogenicity. Consequently, they have the potential to facilitate the therapeutic potential of stem cells, mitigating the risks associated with direct stem cell application. Therefore, the use of stem cell extracellular vesicles in clinical diseases has received increasing attention. This review summarizes advances in the use of extracellular vesicles from mesenchymal stem cells (MSC). MSC extracellular vesicles are used in the treatment of inflammatory diseases such as rheumatoid arthritis, liver injury, COVID-19, and allergies; in the repair of tissue damage in heart disease, kidney injury, and osteoarthritic diseases; as a carrier in the treatment of tumors; and as a regenerative agent in neurodegenerative disorders such as Alzheimer's and Parkinson's.

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“…They can transfer functional RNAs and proteins to target cells, influencing their functional status. Stem cell-derived EVs have been shown to guide chondrogenic differentiation, reduce adipogenesis, and modulate the functions of various cells both in vitro and in vivo [50,51]. Examples of stem cell-secreted EVs include exosomes and microvesicles, which are essential mediators in regenerative medicine and have emerged as promising tools for therapeutic applications.…”

Section: Stem Cell-derived Extracellular Vesicle (Ev) and Stem Cell M...mentioning

confidence: 99%

“…Overall, stem cell-derived EVs have low immunogenicity and strong therapeutic potential for tissue repair and regeneration. They are being explored as a promising alternative to cell-based therapies in regenerative medicine because of their ability to mediate immunomodulation, promote tissue repair, and contribute to the restoration of homeostasis [50,51].…”

Section: Stem Cell-derived Extracellular Vesicle (Ev) and Stem Cell M...mentioning

confidence: 99%

Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery

Matsuzaka,

Yashiro

2024

Pharmaceuticals

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Mesenchymal stem cells (MSCs) have emerged as a promising approach for drug delivery strategies because of their unique properties. These strategies include stem cell membrane-coated nanoparticles, stem cell-derived extracellular vesicles, immunomodulatory effects, stem cell-laden scaffolds, and scaffold-free stem cell sheets. MSCs offer advantages such as low immunogenicity, homing ability, and tumor tropism, making them ideal for targeted drug delivery systems. Stem cell-derived extracellular vesicles have gained attention for their immune properties and tumor-homing abilities, presenting a potential solution for drug delivery challenges. The relationship between MSC-based drug delivery and the self-renewal and differentiation capabilities of MSCs lies in the potential of engineered MSCs to serve as effective carriers for therapeutic agents while maintaining their intrinsic properties. MSCs exhibit potent immunosuppressive functions in MSC-based drug delivery strategies. Stem cell-derived EVs have low immunogenicity and strong therapeutic potential for tissue repair and regeneration. Scaffold-free stem cell sheets represent a cutting-edge approach in regenerative medicine, offering a versatile platform for tissue engineering and regeneration across different medical specialties. MSCs have shown great potential for clinical applications in regenerative medicine because of their ability to differentiate into various cell types, secrete bioactive factors, and modulate immune responses. Researchers are exploring these innovative approaches to enhance drug delivery efficiency and effectiveness in treating various diseases.

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Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation

Cited by 7 publications

References 64 publications

Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells

Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells

Mesenchymal stem cell-derived extracellular vesicles for human diseases

Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery

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