Mesalazine–probiotics beads for acetic acid experimental colitis: formulation and characterization of a promising new therapeutic strategy for ulcerative colitis

Vishwakarma, Neelam; Ganeshpurkar, Aditya; Pandey, Vikas; Dubey, Nazneen; Bansal, Divya

doi:10.3109/10717544.2013.872711

Cited by 13 publications

(9 citation statements)

References 8 publications

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“…The AA-induced colitis model used, is beneficial for the investigation of IBD pathogenesis and novel treatment options for ulcerative colitis, since it induces inflammation and ulceration (7). …”

Section: Discussionmentioning

confidence: 99%

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Effects of dexpanthenol on acetic acid-induced colitis in rats

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et al. 2016

Experimental and Therapeutic Medicine

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While the pathogenesis of acetic acid (AA)-induced colitis is unclear, reactive oxygen species are considered to have a significant effect. The aim of the present study was to elucidate the therapeutic potential of dexpanthenol (Dxp) on the amelioration of colitis in rats. Group I (n=8; control group) was intrarectally administered 1 ml saline solution (0.9%); group II [n=8; AA] was administered 4% AA into the colon via the rectum as a single dose for three consecutive days; group III (n=8; AA + Dxp) was administered AA at the same dosage as group II from day 4, and a single dose of Dxp was administered intraperitoneally; and group IV (n=8; Dxp) was administered Dxp similarly to Group III. Oxidative stress and colonic damage were assessed via biochemical and histologic examination methods. AA treatment led to an increase in oxidative parameters and a decrease in antioxidant systems. Histopathological examination showed that AA treatment caused tissue injury and increased caspase-3 activity in the distal colon and triggered apoptosis. Dxp treatment caused biochemical and histopathological improvements, indicating that Dxp may have an anti-oxidant effect in colitis; therefore, Dxp may be a potential therapeutic agent for the amelioration of IBD.

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Section: Discussionmentioning

confidence: 99%

“…Oxidative destruction is thought to be the pathogenetic factor in this scenario, and this damage is also observed in the gross morphology of the colon (6). It is assumed that the same result occurs in colitis in humans (7). …”

Section: Introductionmentioning

confidence: 99%

Effects of dexpanthenol on acetic acid-induced colitis in rats

Çağın

Parlakpınar

Vardı

et al. 2016

Experimental and Therapeutic Medicine

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“…Process parameters had shown major influence on the physical appearance and micrometric properties (particle size, flow, and compressibility) of the prepared beads [12]. In respect to the results of process parameters, handling capacity of the assembly and primary optimization of process parameters were evaluated based on morphologic studies (appearance and shape).…”

Section: Discussionmentioning

confidence: 99%

Preparation of Budesonide-Pectin Beads Using the Peristaltic Pump

Atara

Soniwala²

2018

Int J Pharm Pharm Sci

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Associate Professor, B. K. Mody Govt. College of Pharmacy, Rajkot, Gujarat, India Email: samir.atara@rku.ac.inObjective: To formulate and evaluate budesonide-pectin beads using novel assembly with a peristaltic pump. Methods:The prepared budesonide-pectin dispersion was added to calcium chloride solution with the help of assembly. Provided at least 2 h for hardening and dried at 60 °C for 3 h. Assembly was assembled with the use of a peristaltic pump, polymeric solution reservoir, hose, needle, magnetic stirrer, gelling bath. One side of hose was dipped in pectin solution container and another side was passed through the peristaltic pump and connected to the needle. Peristaltic pump produced pumping of pectin solution through the hose. Needles with variable diameters are available. The gelling bath was kept on magnetic stirrer to keep prepared beads in motion, which helped to provide new surface and sufficient reaction time and helped to avoid agglomeration. There were 10 different batches with different process parameters prepared. Production yield, the diameter of beads, swelling index, and in vitro budesonide dissolution in phosphate buffer pH 7.4 after 5 h was determined.Results: There was a significant difference between selection of process parameter among the batches (p = 0.049, q = 6.11). As nozzle diameter was increased, percentage yield was decreased (p = 0.0038, q>5.98). However, if hardening time was decreased percentage release was increased (p = 0.0361, q>5.98). Conclusion:The novel assembly for bead generation had developed a uniform, spherical shaped, and smooth surfaced beads.

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“…The properly dried bead was placed on a glass slide which was mounted on the stage of the microscope (Olympus, India). The calibrated ocular micrometre (Olympus, India) was used to measure the diameter of 50 dry beads and mean particle size was calculated [8].…”

Section: Determination Of Average Particle Size Of Beadsmentioning

confidence: 99%

Formulation and Evaluation of Pectin-Calcium Chloride Beads of Azathioprine for Colon Targeted Drug Delivery System

Atara

Soniwala²

2018

Int J Pharm Pharm Sci

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Objective: Calcium pectinate is an insoluble hydrophilic material used for sustained release delivery. The objective of the study was formulation and evaluation pectin-calcium chloride beads of azathioprine.Methods: Calibration curves of azathioprine were prepared in 0.1N HCl solution (pH-1.2) and phosphate buffer (pH-6.8 and pH-7.4). Fourier transform infrared spectroscopy and differential scanning calorimetry was used to determine compatibility between azathioprine and excipients. Formulation and optimization of calcium-pectinate beads were performed. The coating of the optimized batch was performed with eudragit S100. Micrometric properties, scanning electron microscopy, in vitro azathioprine release, and stability study was performed. Dissolution kinetic study was assessed for various kinetics models of the optimized batch. The Wilcoxon test followed by the Dunnett's multiple comparison tests were performed between uncoated and coated beads in vitro dissolution profile of optimized batch in 0.1N HCl after 2 h. Results:Fourier transform infrared spectroscopy and differential scanning calorimetry had identical peaks with that of pure azathioprine in compatibility study. Scanning electron microscopy of an uncoated optimized batch of beads indicated the smooth and uniform surface of prepared beads. Eudragit S100 coating was decreased release of azathioprine release in 0.1N HCl after 2 h (p = 0.049, q = 3.533). Korsmeyer/Peppa's model was applied with release exponent higher than 1. In vitro percentage cumulative azathioprine release was identical before and after stability study. Conclusion:Calcium pectinate beads of azathioprine, a multi-particulate dosage form using pH-dependent approaches were prepared.

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Mesalazine–probiotics beads for acetic acid experimental colitis: formulation and characterization of a promising new therapeutic strategy for ulcerative colitis

Abstract: In conclusion, mesalazine-probiotic loaded beads may serve as a useful colon specific drug delivery system for treatment of colitis.

Cited by 13 publications

References 8 publications

Effects of dexpanthenol on acetic acid-induced colitis in rats

Effects of dexpanthenol on acetic acid-induced colitis in rats

Preparation of Budesonide-Pectin Beads Using the Peristaltic Pump

Formulation and Evaluation of Pectin-Calcium Chloride Beads of Azathioprine for Colon Targeted Drug Delivery System

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