Mesaconitine-induced relaxation in rat aorta: role of Na+/Ca2+ exchangers in endothelial cells

Ogura, Junko; Mitamura, Mana; Someya, Akiyoshi; Shimamura, Ken; Takayama, Hiromitsu; Aimi, Norio; Horie, Syunji; Murayama, Toshihiko

doi:10.1016/j.ejphar.2003.10.022

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…Stimulation of endothelial cells by hyperglycaemia is known to elevate intracellular Ca 2 + concentration ([Ca 2 + ] i ) (Dang et al, 2005). Thereby, Na + /Ca 2 + exchanger contributes to local Ca 2 + homeostasis and cellular control of eNOS activity in the endothelium in rat aorta (Ogura et al, 2004). It was reported that Na + /Ca 2 + exchanger contributed to endothelium-dependent control of vascular contractility and Na + /Ca 2 + exchanger might mediate Ca 2 + influx and be involved in the enhanced [Ca 2 + ]i in the hyperglycemic condition (Kim et al, 2005).…”

Section: Effects Of the Na + /Ca 2 + Exchanger Inhibitor On Biochemicmentioning

confidence: 99%

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Wan

Sun

et al. 2015

Exp Clin Endocrinol Diabetes

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The aims of this study were to examine the effects of KB-R7943, an inhibitor of Na(+)/Ca(2+) exchanger, on the endothelium-dependent relaxation (EDR) in diabetic rat aorta. Both acetylcholine (ACh)-induced EDR and sodium nitroprusside (SNP)-induced endothelium-independent relaxation (EIR) were measured in aortic rings of nondiabetic and streptozotocin-diabetic rats. Diabetes mellitus was induced by single injection of streptozotocin (60 mg/kg). The treated rats received 0.01 or 0.1 mg/kg/day of KB-R7943 for 8 weeks. ACh-induced relaxation was impaired in diabetic compared to control rings and the vasodilatation to SNP was unaffected. Treatment with KB-R7943 markedly enhanced relaxation to ACh in diabetic but not in control rings. KB-R7943 significantly increased superoxide dismutase (SOD) activity and the nitric oxide (NO) release. These results suggest that KB-R7943 can restore impaired EDR in aortic rings of diabetic rats, which may be related to scavenging oxygen free radicals and enhancing NO production.

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Section: Effects Of the Na + /Ca 2 + Exchanger Inhibitor On Biochemicmentioning

confidence: 99%

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Wan

Sun

et al. 2015

Exp Clin Endocrinol Diabetes

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“…The increase in [Ca 2+ ] i is a critical event in influencing numerous Ca 2+ ‐dependent processes in the vascular endothelium, including the synthesis and release of vasoactive substances 23 . Thus, the Na + /Ca 2+ exchanger contributes to local Ca 2+ homeostasis and cellular control of eNOS activity in the endothelium in rat aorta 29 . It was reported that the Na + /Ca 2+ exchanger contributed to endothelium‐dependent control of vascular contractility 30 and that the Na + /Ca 2+ exchanger may mediate Ca 2+ influx and be involved in the enhanced [Ca 2+ ] i under hyperglycaemic conditions 9 …”

Section: Discussionmentioning

confidence: 99%

“…23 Thus, the Na + /Ca 2+ exchanger contributes to local Ca 2+ homeostasis and cellular control of eNOS activity in the endothelium in rat aorta. 29 It was reported that the Na + /Ca 2+ exchanger contributed to endotheliumdependent control of vascular contractility 30 and that the Na + /Ca 2+ exchanger may mediate Ca 2+ influx and be involved in the enhanced [Ca 2+ ] i under hyperglycaemic conditions. 9 In the present study, we found that the beneficial effect of the Na + / Ca 2+ exchanger inhibitor KB-R7943 on endothelial dysfunction elicited by high glucose was similar to the combined effects of SOD and l-arginine, whereas treatment of aortic rings with SOD or l-arginine alone only partly inhibited the impairment of EDR induced by high glucose.…”

Section: Discussionmentioning

confidence: 99%

Na⁺/Ca²⁺ EXCHANGER INHIBITOR AMELIORATES IMPAIRED ENDOTHELIUM‐DEPENDENT Na⁺ RELAXATION INDUCED BY HIGH GLUCOSE IN RAT AORTA

Liu

Sun

et al. 2008

Clin Exp Pharma Physio

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The present study was designed to investigate the effects of KB-R7943, an inhibitor of the Na+/Ca2+ exchanger, on impaired endothelium-dependent relaxation (EDR) induced by high glucose in rat isolated aorta. Both acetylcholine (ACh)-induced EDR and sodium nitroprusside (SNP)-induced endothelium-independent relaxation (EIR) were measured after aortic rings had been exposed to high glucose in the absence and presence of KB-R7943. Coincubation of aortic rings with high glucose (25 mmol/L) for 24 h resulted in a significant inhibition of EDR, but had no effect on EIR. After incubation of aortic rings in the presence of both KB-R7943 (0.1-10 micromol/L) and high glucose for 24 h, significantly attenuation of impaired EDR was observed. This protective effect of KB-R7943 (10 micromol/L) was abolished by superoxide dismutase (SOD; 200 U/mL) and l-arginine (3 mmol/L), whereas d-arginine (3 mmol/L) had no effect. Similarly, high glucose decreased SOD activity and the release of nitric oxide (NO) and increased superoxide anion (O2(-)) production in aortic tissue. KB-R7943 significantly decreased O2(-) production and increased SOD activity and NO release. These results suggest that KB-R7943 can restore impaired EDR induced by high glucose in rat isolated aorta, which may be related to the scavenging of oxygen free radicals and enhanced NO production.

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“…Previous studies have shown that mesaconitine could increase intracellular Ca 2+ concentration levels in human umbilical vein endothelial cells and could cause relaxation in rat aorta via nitric oxide production (Ogura et al, 2004). Aconitine is known to mainly affect the central nervous system and heart (Fu et al, 2007), and improve the cardiovascular function (Sato et al, 1979), but aconitine plays an important role in aconitine-induced tachyarrhythmia in murine atria through changing the expression of receptors and ion channels (Kimura et al, 1994).…”

Section: Screened Bioactive Components Analysismentioning

confidence: 99%

“…The bioactive components related to cardiovascular diseases in the alkaloid extract of Aconitum szechenyianum Gay., a widely used TCM (Shu et al, 2006), were screened by oxidative-damaged endothelial (ECV304) cells, and analyzed with LC-MS. The results showed that at least five compounds in the extract could be captured by oxidative-damaged ECV304 cells, and two identified compounds among them proved to be associated with cardiovascular diseases (Ogura et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

LC‐MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

Yuan

Zhang

Kang

et al. 2008

Biomedical Chromatography

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A novel method of cell affinity screening (CAS), cell affinity capture coupled with LC-MS analysis, was developed for screening the bioactive compounds related to cardiovascular diseases from the natural product libraries. One of the major characteristics lies in its function in affinity-capturing and separating the bioactive components from the natural product libraries in vitro. Another characteristic is its use in analyzing and identifying the target compounds, by employing high-performance liquid chromatography and mass spectrometry. CAS was used for screening the bioactive components from the alkaloid extract derived from Aconitum szechenyianum Gay. Of the five components found to be bound to the oxidative-damaged endothelial cells, the two compounds identified, mesaconitine and aconitine, were recognized in the literature as being related to cardiovascular diseases.

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Mesaconitine-induced relaxation in rat aorta: role of Na+/Ca2+ exchangers in endothelial cells

Cited by 10 publications

References 34 publications

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Na⁺/Ca²⁺ EXCHANGER INHIBITOR AMELIORATES IMPAIRED ENDOTHELIUM‐DEPENDENT Na⁺ RELAXATION INDUCED BY HIGH GLUCOSE IN RAT AORTA

LC‐MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

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Mesaconitine-induced relaxation in rat aorta: role of Na+/Ca2+ exchangers in endothelial cells

Cited by 10 publications

References 34 publications

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Na+/Ca2+ Exchanger Inhibitor Restores Endothelium-dependent Relaxation in Diabetic Rat Aorta

Na+/Ca2+ EXCHANGER INHIBITOR AMELIORATES IMPAIRED ENDOTHELIUM‐DEPENDENT Na+ RELAXATION INDUCED BY HIGH GLUCOSE IN RAT AORTA

LC‐MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

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Na⁺/Ca²⁺ EXCHANGER INHIBITOR AMELIORATES IMPAIRED ENDOTHELIUM‐DEPENDENT Na⁺ RELAXATION INDUCED BY HIGH GLUCOSE IN RAT AORTA