LC‐MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

Abstract

A novel method of cell affinity screening (CAS), cell affinity capture coupled with LC-MS analysis, was developed for screening the bioactive compounds related to cardiovascular diseases from the natural product libraries. One of the major characteristics lies in its function in affinity-capturing and separating the bioactive components from the natural product libraries in vitro. Another characteristic is its use in analyzing and identifying the target compounds, by employing high-performance liquid chromatography and mass spectrometry. CAS was used for screening the bioactive components from the alkaloid extract derived from Aconitum szechenyianum Gay. Of the five components found to be bound to the oxidative-damaged endothelial cells, the two compounds identified, mesaconitine and aconitine, were recognized in the literature as being related to cardiovascular diseases.

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…In a similar way, Ren et al adopted prostate cell membrane chromatography hyphenated with LC-MS to identify three bisbenzylisoquinoline alkaloids acting on ␣ 1A -adrenoceptor [110]. In addition, Yuan et al reported on a cell affinity capture coupled with LC-MS method for the screening of bioactive components of Aconitum szechenyianum [111]. Alkaloids of C. yanhusuo were screened to find potential aromatase binding agents using Reproduced with permission from [92].…”

Applications of HPLC/MS in the analysis of traditional Chinese medicines

“…Jong et al [12] pres-ented an HPLC/MS methodology for the screening of ace-tylcholinesterase (AChE) inhibitors in a crude extract of Narcissus cv “Bridal Crown” bulbs. Yuan et al [13] cou-pled cell affinity screening (CAS) with HPLC/MS to screen the bioactive compounds related to cardiovascular diseases from the alkaloid extract derived from Aconitum szecheny-ianum Gay.…”

C19-Norditerpenoid Alkaloids from Aconitum szechenyianum

“…C 19 - and C 20 -diterpenoid alkaloids possessing aconitine-type, 7,17-secoaconitine-type, and napeline-type skeletons, which are the main components of A . szechenyianum [ 2 , 3 , 4 , 5 ], possess anti-inflammatory, analgesic, anticancer, anti-epileptiform, and antiparasitic activities [ 6 , 7 , 8 ]. A. szechenyianum has been preliminarily studied; in that study, several norditerpenoid alkaloids were obtained with an aconitine or 7,17-secoaconitine skeleton, and these skeleton were demonstrated to have anti-inflammatory activities in a dose-dependent manner [ 9 ].…”