Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis

Ito, Masaki; Sato, Tsutomu; Fukuda, Hiroo; Komamine, Atsushi

doi:10.1007/bf00014441

Cited by 10 publications

(5 citation statements)

References 50 publications

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Section: Discussionmentioning

confidence: 99%

“…Whereas OCEs and Octs are highly conserved among histone genes, they are rarely found in other genes, including those which are (expected to be) expressed at the G1/S boundary and S phase, such as the PCNA gene (Kosugi et al, 1991) and the cyc07 gene (Ito et al, 1994). Recently, we found a type I-like element in the promoters of a gene encoding the non-histone chromatin protein HMG-I/Y (Gupta et al, 1998) and a related gene (G. Morisawa et al, unpublished data).…”

Section: Oce-mediated Regulation Of Histone Genes (A Model)mentioning

confidence: 99%

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Identification of three kinds of mutually related composite elements conferring S phase‐specific transcriptional activation

et al. 1999

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Summary Conservation of the Oct motif (CGCGGATC) is a remarkable feature of plant histone gene promoters. Many of the Oct motifs are paired with a distinct motif, Hex, TCA or CCAAT‐box, constituting the type I element (CCACGTCANCGATCCGCG), type II element (TCACGCGGATC) and type III element (GATCCGCG‐N14‐ACCAATCA). To clarify the roles of these Oct‐containing composite elements (OCEs) in cell cycle‐dependent and tissue‐specific expression, we performed gain‐of‐function experiments with transgenic tobacco cell lines and plants harboring a derivative of the 35S core promoter/β‐glucuronidase fusion gene in which three or four copies of an OCE had been placed upstream. Although their activities were slightly different, results showed that each of the three types of OCEs could confer the ability to direct S phase‐specific expression on a heterologous promoter. In transgenic plants, the type I and III elements exhibited a similar activity, directing expression in meristematic tissues, whereas the activity of the type II element appeared to be restricted to young cotyledons and maturating guard cells. Mutational analyses demonstrated that the co‐operation of Oct with another module (Hex, TCA or CCAAT‐box) was absolutely required for both temporal and spatial regulation. Thus, OCEs play a pivotal role in regulation of the expression of plant histone genes.

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Section: Discussionmentioning

confidence: 99%

Section: Oce-mediated Regulation Of Histone Genes (A Model)mentioning

confidence: 99%

Identification of three kinds of mutually related composite elements conferring S phase‐specific transcriptional activation

et al. 1999

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“…Transcriptional start sites of CYS and CYM were determined by primer extension analysis using oligonucleotide primers corresponding to the nucleotide positions 23-50 of the CYS cDNA and 20-48 of the CYMcDNA. Primer extension analysis was performed as described previously (Ito et al, 1994).…”

Section: Determination Of Transcriptional Start Sitesmentioning

confidence: 99%

Cell‐cycle‐regulated transcription of A‐ and B‐type plant cyclin genes in synchronous cultures

et al. 1997

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SummarySynchronously dividing cell cultures of Catharanthus roseus were used to isolate cDNAs for two mitotic cyclins, named CYS and CYM. The deduced protein sequence of CYS is similar to that of A-type cyclins, and CYM belongs to the group of B-type cyclins. In a fashion similar to the pattern of expression seen for A-type and B-type cyclins in mammalian cells, CYS is expressed before CYM in C. roseus cells during the cell cycle. CYS mRNA accumulated at the onset of S phase and disappeared early in the G2 phase, whereas CYM mRNA was detected in the G2 and M phases of the cell cycle. Tobacco homologs of the two genes showed similar cell-cycle dependent expression patterns in synchronous cultures of tobacco BY2 cells. In both systems, CYS was expressed much earlier in the cell cycle than most other plant A-type cyclins, and hence CYS along with the soybean cyclGm can be classified into a distinct subclass. The activities of CYMand CYS promoters during the cell cycle were analyzed in stably transformed tobacco BY2 cells. Cyclin promoter sequences of 0.5 kb could confer the typical cell-cycle-dependent expression to the ~-glucuronidase (GUS) reporter gene: the CYS Received 15 July 1996; revised 20 December 1996; accepted 17 January 1997. *For correspondence (fax +81 3 3814 1728; e-mail masakito@uts2.s.u-tokyo.ac.jp). tPresent address: Laboratoire de Biologie Generale, CNRS Universite Louis Pasteur, 12 Rue de I'Universite, 67000 Strasbourg, France. tPresent address: Botanical Gardens, Faculty of Science, University of Tokyo, Hakusan, Tokyo, 112 Japan.promoter directed S-phase-specific expression, whereas the CYM promoter drove M-phase-specific expression. These results indicate the important role of transcriptional regulation in the oscillations of cyclin mRNA levels during the cell cycle.

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“…C. roseus cyc07 is expressed mainly in the meristematic regions of transgenic Arabidopsis [31]. In addition, cyc07 expression is regulated by environmental stress,including high osmotic pressure, salinity, low temperature and submergence in Oryza sativa [27].…”

Section: Cloning and Characterization Of The Tobacco Gene Ntcyc07mentioning

confidence: 99%

“…Cyc07 genes from Catharanthus roseus [25] and S. cerevisiae [26] have been reported to be expressed in S phase and their products have been proposed to play a role in cell proliferation. C. roseus cyc07 is expressed mainly in the meristematic regions of transgenic Arabidopsis [31]. In addition, cyc07 expression is regulated by environmental stress,including high osmotic pressure, salinity, low temperature and submergence in Oryza sativa [27].…”

Section: Cloning and Characterization Of The Tobacco Gene Ntcyc07mentioning

confidence: 99%

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

et al. 2008

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We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.

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Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis

Cited by 10 publications

References 50 publications

Identification of three kinds of mutually related composite elements conferring S phase‐specific transcriptional activation

Identification of three kinds of mutually related composite elements conferring S phase‐specific transcriptional activation

Cell‐cycle‐regulated transcription of A‐ and B‐type plant cyclin genes in synchronous cultures

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

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