Merging Preclinical EPR Tomography with other Imaging Techniques

Gonet, Michał; Epel, Boris; Halpern, Howard J.; Elas, Martyna

doi:10.1007/s12013-019-00880-7

Cited by 7 publications

(3 citation statements)

References 64 publications

(49 reference statements)

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“…Absolute oxygen levels, reactive oxygen species (ROS), oxidative stress or spin probes can be determined in vivo by this method [42]. Magnetic molecular methods are usually co-registered with MRI or CT [43].…”

Section: Preclinical Hybrid Imagingmentioning

confidence: 99%

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

et al. 2020

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Section: Preclinical Hybrid Imagingmentioning

confidence: 99%

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

et al. 2020

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“…Therefore, the noninvasive imaging of free radicals in living organisms is considered as a crucial metabolic and functional imaging method for diagnosis as well as drug development. , The development of in vivo electron spin resonance (ESR) spectroscopy and ESR imaging has resulted in the involvement of in vivo redox processes in various oxidative stress-mediated pathologies, wherein exogenous stable radicals have been utilized as tracers . Although the mapping of oxygen concentration, pH, and redox state has been performed considering model animals, − a very few clinical studies on free radical imaging using ESR imaging have been reported …”

Section: Introductionmentioning

confidence: 99%

Development and Preclinical Study of Free Radical Imaging Using Field-Cycling Dynamic Nuclear Polarization MRI

et al. 2021

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Free radicals, such as metabolic intermediates, reactive oxygen species, and metal enzymes, are key substances in organisms, although they can also cause various oxidative diseases. Thus, in vivo free radical imaging should be considered as the ultimate form of metabolic imaging. Unfortunately, electron spin resonance (ESR) imaging has inherent disadvantages, such as free radicals with large linewidths generating blurred images and the presence of two or more free radicals resulting in a complicated imaging procedure. Dynamic nuclear polarization−magnetic resonance imaging (DNP-MRI) is a noninvasive imaging method to visualize in vivo free radicals, theoretically, with the same resolution as the MRI anatomical resolution, and fixed low-field DNP-MRI provides unique information on oxidative diseases and cancer. However, the large gyromagnetic ratio of the electron spin, which is 660-fold greater than that of a proton, requires field cycling, wherein the external magnetic field should be varied during DNP-MRI observations. This causes difficulties in developing a DNP-MRI system for clinical purposes. We developed a novel field-cycling DNP-MRI system for a preclinical study. In the said system, the magnetic field is switched by rotationally moving two magnets, with a magnetic flux density of 0.3 T for MRI and 5 mT for ESR. The image quality was examined using various pulse sequences and ESR irradiation using nitroxyl radical as the phantom, and the optimum conditions were established. Using the system, we performed a preclinical study involving free radical imaging by placing the free radicals under the palm of a human hand.

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“…Functional electron paramagnetic resonance (EPR) imaging has been successfully used in a wide range of biomedical studies [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Besides the distribution of spin probes, spatial distributions of several tissue microenvironment markers, such as oxygen partial pressure, pH or redox status have been measured [2,5,[15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Rapid Scan EPR Imaging as a Tool for Magnetic Field Mapping

2020

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Functional four-dimensional spectral-spatial electron paramagnetic imaging (EPRI) is routinely used in biomedical research. Positions and widths of EPR lines in the spectral dimension report oxygen partial pressure, pH, and other important parameters of the tissue microenvironment. Images are measured in the homogeneous external magnetic field. An application of EPRI is proposed in which the field is perturbed by a magnetized object. A proof-of-concept imaging experiment was conducted, which permitted visualization of the magnetic field created by this object. A single-line lithium octa-n-butoxynaphthalocyanine spin probe was used in the experiment. The spectral position of the EPR line directly measured the strength of the perturbation field with spatial resolution. A three-dimensional magnetic field map was reconstructed as a result. Several applications of this technology can be anticipated. First is EPRI/MPI co-registration, where MPI is an emerging magnetic particle imaging technique. Second, EPRI can be an alternative to magnetic field cameras that are used for the development of high-end permanent magnets and their assemblies, consumer electronics, and industrial sensors. Besides the high resolution of magnetic field readings, EPR probes can be placed in the internal areas of various assemblies that are not accessible by the standard sensors. Third, EPRI can be used to develop systems for magnetic manipulation of cell cultures.

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Merging Preclinical EPR Tomography with other Imaging Techniques

Cited by 7 publications

References 64 publications

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

Development and Preclinical Study of Free Radical Imaging Using Field-Cycling Dynamic Nuclear Polarization MRI

Rapid Scan EPR Imaging as a Tool for Magnetic Field Mapping

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