Mercaptolated chitosan/methacrylate gelatin composite hydrogel for potential wound healing applications

Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for In Vitro Cell Culture Systems

“…Owing to these attributes, there has been large interest in the last decade about the preparation of gelatin hydrogels introducing thiol reactivity toward electron-rich/electron-poor carbon–carbon double bonds. − Some reports have been published considering the research in which gelatin contains the thiol moiety, , although there is a wider variety in case gelatin contains the double-bond. − Thus, thiol-based click chemistry using norbornene-functionalized gelatin has been thoroughly described − and mostly applied in biofabrication. − As for the thiol-methacrylamide system, few studies have been found and only macromer concentration has been deeply investigated about its influence on mechanical properties and cell response . In those relevant studies, either the reaction involves a macromer also on the thiol sidegelatin, heparine, lignosulfonateor the thiol is added after the acrylate photopolymerization has taken place. − Other researchers have used in the same material crosslinking reactionsthiol-yne or dynamic covalent chemistryadditional to acrylate photopolymerization and thiol-ene reaction, which make interpretation of the structure–property relationships more challenging. , …”

“… 44 − 47 Other researchers have used in the same material crosslinking reactions—thiol-yne or dynamic covalent chemistry—additional to acrylate photopolymerization and thiol-ene reaction, which make interpretation of the structure–property relationships more challenging. 44 , 48 …”

“…44−47 Other researchers have used in the same material crosslinking reactions�thiol-yne or dynamic covalent chemistry�additional to acrylate photopolymerization and thiol-ene reaction, which make interpretation of the structure−property relationships more challenging. 44,48 Herein, we present a platform of GelMA-based photopolymerizable hydrogels with tunable mechanical properties for culture of human colon carcinoma cell line (Caco-2). We have generated scaffolds for in vitro intestinal models using on one hand hydrogels prepared by free-radical photopolymerization of GelMA.…”

Enzymatically crosslinked hydrogel based on tyramine modified gelatin and sialylated chitosan

“…Among the various methods of hydrogel preparation, enzyme-mediated crosslinking of chemically formed hydrogels has attained much attention due to its high substrate specificity and efficacy [51]. Importantly, unlike other chemical cross-linking reactions that involve photoinitiators [52] and organic solvents [53,54], there is no risk of unwanted side effects or toxicity in the enzymatic hydrogelation systems. Horseradish peroxidase (HRP) is one of the enzymes that has been used to catalyze the cross-linking of phenol or aniline derivatives in the presence of H 2 O 2 [55,56].…”

Graphene oxide-reinforced alginate/gelatin hydrogel via Schiff-base bond and thiol-Michael addition for bone regeneration

“…Click chemistry is characterized by high reaction efficiency, high selectivity, and processing simplicity [9,10]. Indeed, the literature reports that several click chemistries have been employed to design new hydrogels with improved properties, such as azide and alkyne cycloaddition, thiol-ene/yne addition, oxime-based click reactions, and other click reactions [9,11]. For instance, Pupkaite et al, [12] modified collagen using thiol groups, and prepared injectable collagen hydrogels by cross-linking with 8-arm polyethylene glycol-maleimide using the thiol-Michael addition click reaction.…”