Enzymatically crosslinked hydrogel based on tyramine modified gelatin and sialylated chitosan

Abstract

The enzymatically crosslinked hydrogel could replicate the cellular microenvironment for biomedical applications. In the present study, to improve the cytocompatibility of chitosan (CS), sialic acid (SA) was introduced to CS to synthesize sialylated CS (CS-SA), and the tyramine (TA) was grafted to gelatin (G) to obtain TA modified gelatin (G-TA). The successful synthesis of CS-SA and G-TA was confirmed using 1H NMR and UV-Vis absorption spectra. The interpenetrating polymer networks (IPN) G-TA/CS-SA (GC) hydrogel was then fabricated via blending G-TA and CS-SA solutions and crosslinked using horseradish peroxidase (HRP). The storage modulus (G’) of the fabricated GC hydrogels with different ratios of G-TA/CS-SA greatly varied during the formation and strain of hydrogels. With the increase of CS-SA concentration from 0 % to 2 %, the storage modulus of GC hydrogels was also observed to decrease from 1500 Pa to 101 Pa; the water uptake capacity of GC hydrogels increased from 1000 % to 4500 %. Additionally, the cell counting kit-8 (CCK-8) and fluorescent images demonstrated the excellent cytocompatibility of GC hydrogels after culturing with NIH 3T3 cells. The obtained results indicated that the fabricated GC hydrogels might have potential in biomedical fields, such as wound healing dressing.

Bioactive wound dressing based on decellularized tendon and GelMA with incorporation of PDA-loaded asiaticoside nanoparticles for scarless wound healing

“…Next, the rheological properties of hydrogels were further investigated, and the storage moduli (G') and loss moduli (G'') of hydrogels were tested in terms of temperature and frequency (Figures 3H and 3I). The results show that G' of all obtained hydrogels is greater than G'', exhibiting the typical viscous flow characteristics of hydrogel [39,59,60]. There was a slight increase in both G' and G'' with the increase of temperature from -4 °C to 60 °C.…”

Self-adhesive and self-healing hydrogel dressings based on quaternary ammonium chitosan and host-guest interacted silk fibroin

“…These results indicate that the hydrogels possessed good biocompatibility. In our previous research [25,46,47], SF and chitosan were used to fabricate hydrogels dressings for wound healing application by compositing with other polymers or loading antibiotic drugs. In this work, the hydrogel dressings could be designed and obtained based on the host-guest interactions and hydrogen-bonding assembly from SF and chitosan.…”

A Novel Nanofiber Hydrogel Adhesive Based on Carboxymethyl Cellulose Modified by Adenine and Thymine

“…According to the report, different EDCI/NHS and buffer systems were considered [35][36][37][38][39]. It was found that when the ratio of NHS to EDCI exceeded one, there was almost no unreacted adenine and thymine.…”