“…A limited number of studies indicate that once microorganisms are exposed to excess hydrogen, hydrogen turnover will not further increase with increasing hydrogen pressure 68,69 , indicating that hydrogen turnover rates determined at excess hydrogen and standard conditions may be representative and applicable to estimate subsurface hydrogen rates, providing realistic nutrient, temperature, pressure, and salinity regimes are applied. A number of classes of microorganisms, including methanogens, sulphate-reducers, homo-acetogenic bacteria and iron(III)-reducers are considered as major hydrogen consumers and are frequently present in subsurface formations 70 . The potential impact of the microorganisms is controlled by parameters such as temperature, salt concentration, pH-value, and substrate supply, with optimal and critical values for these parameters for each microorganism summarised in Table 1.…”