Memory formation and retention are affected in adult miR-132/212 knockout mice

Hernandez-Rapp, Julia; Smith, Pascal Y.; Filali, Mohammed; Goupil, Claudia; Planel, Emmanuel; Magill, Stephen T.; Goodman, Richard H.; Hébert, Sébastien S.

doi:10.1016/j.bbr.2015.03.032

Cited by 82 publications

(75 citation statements)

References 72 publications

(83 reference statements)

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“…8 In fact, miRNAs are estimated to regulate as much as 60% of human genes. 19 MicroRNA-132 (miR-132) has been reported to be downregulated in the central nervous system (CNS) of patients suffering from dementia, 20 whereas ACHE was found to be upregulated in these patients. 10,11 Therefore, it is not surprising to discover a close association between miRNAs and neurodegenerative diseases such as Parkinson disease, 12 aggressive behaviors, 13 depression, 14 schizophrenia, 15 and Alzheimer's disease.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Upregulation of acetylcholinesterase caused by downregulation of microRNA‐132 is responsible for the development of dementia after ischemic stroke

Yang

Wang

et al. 2019

J of Cellular Biochemistry

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has been shown to participate in many diseases. This study aimed to understand the correlation between the level of miR-132 and the severity of dementia post-ischemic stroke. An online tool (www.mirdb.org) was used to find the miR-132 binding site in acetylcholinesterase (ACHE) 3′-untranslated region (UTR), followed by a luciferase reporter assay to validate ACHE as a miR-132 target. A similar relationship between miR-132 and ACHE was also established in cerebrospinal fluid samples collected from human subjects. A negative correlation was established between ACHE and miR-132 by measuring the relative luciferase activity. Meanwhile, Western blot analysis and real-time polymerase chain reaction were also conducted to compare the levels of ACHE messenger RNA and protein between two groups (dementia positive, n = 26 and dementia negative, n = 26) or among cells treated with miR-132 mimics, ACHE small interfering RNA, and miR-132 inhibitors. As shown in the results, miR-132 can reduce the expression of ACHE. Further experiments were also carried out to study the effect of miR-132 and ACHE on cell viability and apoptosis, and the results demonstrated that miR-132 enhanced cell viability while suppressing apoptosis. In addition, ACHE reduced cell viability while promoting apoptosis. miR-132 targeted ACHE and suppressed its expression. Additionally, miR-132 and ACHE have been shown to affect the cell viability and apoptosis in the central nervous system. K E Y W O R D Sacetylcholinesterase, CSF, dementia, ischemic stroke, microRNA-132

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Section: Introductionmentioning

confidence: 99%

Retracted: Upregulation of acetylcholinesterase caused by downregulation of microRNA‐132 is responsible for the development of dementia after ischemic stroke

Yang

Wang

et al. 2019

J of Cellular Biochemistry

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“…We show that these changes are paralleled by a considerable transcriptome rearrangement: many miRNAs undergo a remarkable change of expression between P10 and P28 with the top hits being miR-29, that was previously involved in regulating epigenetic enzymes important for cortical plasticity6; miR-338 and miR-219, that were suggested to be key players in myelination4243; and miR-132/212, a miRNA family previously involved in synaptic plasticity8910112644454647. The combined analysis of miRNAs and mRNAs in the visual cortex of wt and miR-132/212 null mice revealed that genes downregulated with age and upregulated by miR-132/212 deletion are highly enriched with miR-132-3p targets and, to a much lesser extent, with targets of miR-212-5p, the only two members of the miR-132 family abundantly expressed in the visual cortex.…”

Section: Discussionmentioning

confidence: 95%

Mir-132/212 is required for maturation of binocular matching of orientation preference and depth perception

et al. 2017

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MicroRNAs (miRNAs) are known to mediate post-transcriptional gene regulation, but their role in postnatal brain development is still poorly explored. We show that the expression of many miRNAs is dramatically regulated during functional maturation of the mouse visual cortex with miR-132/212 family being one of the top upregulated miRNAs. Age-downregulated transcripts are significantly enriched in miR-132/miR-212 putative targets and in genes upregulated in miR-132/212 null mice. At a functional level, miR-132/212 deletion affects development of receptive fields of cortical neurons determining a specific impairment of binocular matching of orientation preference, but leaving orientation and direction selectivity unaltered. This deficit is associated with reduced depth perception in the visual cliff test. Deletion of miR-132/212 from forebrain excitatory neurons replicates the binocular matching deficits. Thus, miR-132/212 family shapes the age-dependent transcriptome of the visual cortex during a specific developmental window resulting in maturation of binocular cortical cells and depth perception.

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“…Moreover, miR‐128‐3p has been shown to govern motor activity by suppressing the expression of various ion channels and mitogen‐activated protein kinase 1 (ERK2) signaling components and its overexpression attenuates motor abnormalities associated with Parkinson's‐like disease and seizures in mice . MMSE was negatively correlated with miR‐132‐3p, an miRNA that has been shown to regulate dopamine neuron differentiation by directly targeting Nurr1 mRNA and strongly linked to memory formation and retention . Furthermore, when PD patients were separated into normal/abnormal cognition, a statistically significant decrease in miR‐7‐5p expression levels was identified for total PD and SNCA A53T PD in patients with normal cognition.…”

Section: Discussionmentioning

confidence: 99%

Circulating Brain‐Enriched MicroRNAs for Detection and Discrimination of Idiopathic and Genetic Parkinson's Disease

et al. 2019

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Background A minimally invasive test for early detection and monitoring of Parkinson's disease (PD) is a highly unmet need for drug development and planning of patient care. Blood plasma represents an attractive source of biomarkers. MicroRNAs (miRNAs) are conserved noncoding RNA molecules that serve as posttranscriptional regulators of gene expression. As opposed to ubiquitously expressed miRNAs that control house‐keeping processes, brain‐enriched miRNAs regulate diverse aspects of neuron development and function. These include neuron‐subtype specification, axonal growth, dendritic morphogenesis, and spine density. Backed by a large number of studies, we now know that the differential expression of neuron‐enriched miRNAs leads to brain dysfunction. Objectives The aim was to identify subsets of brain‐enriched miRNAs with diagnostic potential for familial and idiopathic PD as well as specify the molecular pathways deregulated in PD. Methods Initially, brain‐enriched miRNAs were selected based on literature review and validation studies in human tissues. Subsequently, real‐time reverse transcription polymerase chain reaction was performed in the plasma of 100 healthy controls and 99 idiopathic and 53 genetic (26 alpha‐synucleinA53T and 27 glucocerebrosidase) patients. Statistical and bioinformatics analyses were carried out to pinpoint the diagnostic biomarkers and deregulated pathways, respectively. Results An explicit molecular fingerprint for each of the 3 PD cohorts was generated. Although the idiopathic PD fingerprint was different from that of genetic PD, the molecular pathways deregulated converged between all PD subtypes. Conclusions The study provides a group of brain‐enriched miRNAs that may be used for the detection and differentiation of PD subtypes. It has also identified the molecular pathways deregulated in PD. © 2019 International Parkinson and Movement Disorder Society

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Memory formation and retention are affected in adult miR-132/212 knockout mice

Cited by 82 publications

References 72 publications

Retracted: Upregulation of acetylcholinesterase caused by downregulation of microRNA‐132 is responsible for the development of dementia after ischemic stroke

Retracted: Upregulation of acetylcholinesterase caused by downregulation of microRNA‐132 is responsible for the development of dementia after ischemic stroke

Mir-132/212 is required for maturation of binocular matching of orientation preference and depth perception

Circulating Brain‐Enriched MicroRNAs for Detection and Discrimination of Idiopathic and Genetic Parkinson's Disease

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