Membranes of chitosan grafted onto poly(3-hydroxybutyrate): new insights into their applicability as scaffolds

Quintanilla, Francisco; Rodríguez, José Javier Polo; Vargas, Susana; Solis, Scott; Olayo, Roberto; Hnatchuk, Nathalie; Brostow, Witold; Chen, IKang; González, Martha Letícia Gaeta

doi:10.1080/14328917.2015.1109198

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…The latest area exhibited a rounded geometry and suggested a random non-directional pore structure, which ranges in size, of approximately 1 ± 0.14 μm. Therefore, owing the large degree of interconnectivity and the wide range of macro-porosity, it appears that the P(3HB-g-VA) polyurethane scaffold may be suitable for the cell growth and proliferation 12 16 22 25 28 31 42 43 44 45 46 47 48 49 . On the other hand, the result of the mechanical properties of P(3HB-g-VA) polyurethane scaffolds is consistent with that obtained for the synthesis of poly(3-hydroxybutyrate) and nanohydroxyapatite thermal remolded composite scaffold and three-dimensional scaffolds prepared from lyophilized poly(3-hydroxybutyrate-co-hydroxyhexanoate) 44 49 .…”

Section: Resultsmentioning

confidence: 99%

Novel Poly(3-hydroxybutyrate-g-vinyl alcohol) Polyurethane Scaffold for Tissue Engineering

Reyes

Torres

Castro

et al. 2016

Sci Rep

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The design of new synthetic grafted poly(3-hydroxybutyrate) as composite 3D-scaffolds is a convenient alternative for tissue engineering applications. The chemically modified poly(3-hydroxybutyrate) is receiving increasing attention for use as biomimetic copolymers for cell growth. As of yet, these copolymers cannot be used efficiently because of the lack of good mechanical properties. Here, we address this challenge, preparing a composite-scaffold of grafted poly(3-hydroxybutyrate) polyurethane for the first time. However, it is unclear if the composite structure and morphology can also offer a biological application. We obtained the polyurethane by mixing a polyester hydroxylated resin with polyisocyanate and the modified polyhydroxyalkanoates. The results show that the poly(3-hydroxybutyrate) grafted with poly(vinyl alcohol) can be successfully used as a chain extender to form a chemically-crosslinked thermosetting polymer. Furthermore, we show a proposal for the mechanism of the polyurethane synthesis, the analysis of its morphology and the ability of the scaffolds for growing mammalian cells. We demonstrated that astrocytes isolated from mouse cerebellum, and HEK293 can be cultured in the prepared material, and express efficiently fluorescent proteins by adenoviral transduction. We also tested the metabolism of Ca2+ to obtain evidence of the biological activity.

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