In this study, we fabricated a flexible, stretchable glucose-biofuel cell with silver nanowires (AgNWs) on a dimethylpolysiloxane substrate. The biofuel cell investigated consists of a porous carbon anode (area of 30 mm 2 ) modified by glucose oxidase and ferrocene, and a cathode (area of 30 mm 2 ) modified by bilirubin oxidase. The anode and the cathode were connected with AgNWs. The maximum power of 0.29 μW at 180 mV, which corresponds to a power density of 0.98 μW/cm 2 , is realized by immersing the biofuel cell in a phosphate buffer solution with a glucose concentration of 100 mM, at room temperature.