An enzymatic biofuel cell based on electrically wired polyphenol oxidase and glucose oxidase operating under physiological conditions

Giroud, Françoise; Gondran, Chantal; Gorgy, Karine; Vivier, Vincent; Cosnier, Serge

doi:10.1016/j.electacta.2012.08.072

Cited by 23 publications

(10 citation statements)

References 30 publications

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“…Since a dialysis bag was needed to prevent the leaching of ubiquinone, the authors replaced ubiquinone with tetrathiafulvalene. 643 An original electrode has also been developed by Wang et al A mixture of banana pulp, rich in PPO, and graphite particles, was used to elaborate a biocathode. In presence of hydroquinone, as diffusing redox mediator, the authors were able to reach 0.27 mA.cm -2 in 0.1 M PBS pH 7.4.…”

Section: Polyphenol Oxidasementioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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Catalytic four-electron reduction of O to water is one of the most extensively studied electrochemical reactions due to O exceptional availability and high O/HO redox potential, which may in particular allow highly energetic reactions in fuel cells. To circumvent the use of expensive and inefficient Pt catalysts, multicopper oxidases (MCOs) have been envisioned because they provide efficient O reduction with almost no overpotential. MCOs have been used to elaborate enzymatic biofuel cells (EBFCs), a subclass of fuel cells in which enzymes replace the conventional catalysts. A glucose/O EBFC, with a glucose oxidizing anode and a O reducing MCO cathode, could become the in vivo source of electricity that would power sometimes in the future integrated medical devices. This review covers the challenges and advances in the electrochemistry of MCOs and their use in EBFCs with a particular emphasis on the last 6 years. First basic features of MCOs and EBFCs are presented. Clues provided by electrochemistry to understand these enzymes and how they behave once connected at electrodes are described. Progresses realized in the development of efficient biocathodes for O reduction relying both on direct and mediated electron transfer mechanism are then discussed. Some implementations in EBFCs are finally presented.

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Section: Polyphenol Oxidasementioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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“…The open circuit voltage of the glucose biofuel cell operating in 20 mM glucose was 530 mV, which is very similar to the potential difference at which glucose oxidation and oxygen reduction start to occur in the cyclic voltammogram under air-saturated environment, which is comparable or superior to those previously reported using GOx or PQQ-GDH (Miyake et al, 2013;MacVittie et al, 2013;Halamkova et al, 2012). The high open circuit voltage could be attributed to the fact that the oxidation of glucose by PQQ-GDH does not produce hydrogen peroxide in contrast to GOx or mixture of various enzymes (Sales et al, 2013;Rasmussen et al, 2012;Giroud et al, 2012;Cinquin et al, 2010;Barriere et al, 2006). The production of hydrogen peroxide as a byproduct in glucose oxidation reaction leads to the denaturing of the enzyme.…”

Section: Glucose Biofuel Cell Characterizationmentioning

confidence: 99%

A self-powered glucose biosensing system

Slaughter

Kulkarni

2016

Biosensors and Bioelectronics

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A self-powered glucose biosensor (SPGS) system is fabricated and in vitro characterization of the power generation and charging frequency characteristics in glucose analyte are described. The bioelectrodes consist of compressed network of three-dimensional multi-walled carbon nanotubes with redox enzymes, pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and laccase functioning as the anodic and cathodic catalyst, respectively. When operated in 45 mM glucose, the biofuel cell exhibited an open circuit voltage and power density of 681.8 mV and 67.86 µW/cm(2) at 335 mV, respectively, with a current density of 202.2 µA/cm(2). Moreover, at physiological glucose concentration (5mM), the biofuel cell exhibits open circuit voltage and power density of 302.1 mV and 15.98 µW/cm(2) at 166.3 mV, respectively, with a current density of 100 µA/cm(2). The biofuel cell assembly produced a linear dynamic range of 0.5-45 mM glucose. These findings show that glucose biofuel cells can be further investigated in the development of a self-powered glucose biosensor by using a capacitor as the transducer element. By monitoring the capacitor charging frequencies, which are influenced by the concentration of the glucose analyte, a linear dynamic range of 0.5-35 mM glucose is observed. The operational stability of SPGS is monitored over a period of 63 days and is found to be stable with 15.38% and 11.76% drop in power density under continuous discharge in 10mM and 20mM glucose, respectively. These results demonstrate that SPGSs can simultaneously generate bioelectricity to power ultra-low powered devices and sense glucose.

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“…To our knowledge, the highest power density to date for a GOx-based DET BFC (1.25 mW·cm −2 ) was shown for a BFC composed of a GOx/Catalase-CNT bioanode and Laccase-CNT biocathode where both the anode and cathode were generated using mechanical compression methods [16]. When compared to BFCs employing MET strategies, the peak power density output of the current BFC using a 100% GOx CEC-CNT bioanode is greater than other studies of GOx bioanodes [21, 32, 35, 37, 63] (Table 1). The highest MET-based power density output of a non-clustered GOx bioanode is 350 μW·cm −2 for A. niger GOx [64] and 280 μW·cm −2 using Penicillium pinophilum GOx [65].…”

Section: Resultsmentioning

confidence: 99%

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

Dudzik¹,

Chang²,

Kannan³

et al. 2013

Biofabrication

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The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Crosslinked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx in solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. STEM analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed CEC clusters of ~78 μm2 localized that the CNT surface. Electrochemical analysis indicates that the enzyme is engaging in direct electron transfer, and BFCs generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ~180 μW·cm−2. These data indicate that the generation of nano-to-micro sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.

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An enzymatic biofuel cell based on electrically wired polyphenol oxidase and glucose oxidase operating under physiological conditions

Abstract: enzymatic biofuel cell based on electrically wired polyphenol oxidase and glucose oxidase operating under physiological conditions. Electrochimica Acta, Elsevier, 2012, 85 (15)

Cited by 23 publications

References 30 publications

O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells

A self-powered glucose biosensing system

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

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An enzymatic biofuel cell based on electrically wired polyphenol oxidase and glucose oxidase operating under physiological conditions

Abstract: enzymatic biofuel cell based on electrically wired polyphenol oxidase and glucose oxidase operating under physiological conditions. Electrochimica Acta, Elsevier, 2012, 85 (15)

Cited by 23 publications

References 30 publications

O2Reduction in Enzymatic Biofuel Cells

O2Reduction in Enzymatic Biofuel Cells

A self-powered glucose biosensing system

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

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O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells