Membrane stability and methaemoglobin content of human erythrocytes incubated in aqueous leaf extract of Nicotiana tabacum product

Chikezie, Paul Chidoka; Akuwudike, A.R.; Chikezie, C.

doi:10.5530/ax.2012.4.10

Cited by 5 publications

(5 citation statements)

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“…A bioactive secondary metabolite profile is related to various factors, namely, tobacco varieties, growing regions, or fields with varying altitudes, latitudes, and ultraviolet radiation, age, and the organs analyzed [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. Moreover, both the method and solvents, as well as the conditions of extraction of plant biomass play a key role in the chemical composition of extracts, and consequently in its biological activity.…”

Section: Resultsmentioning

confidence: 99%

“…The chemical studies revealed that tobacco leaves are rich in sterols, diterpenoids, sesquiterpenes, alkaloids (including nicotine), and phenolic compounds i.e., flavonoids, phenolic acids, and coumarins [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Hence, tobacco leaves may well be an important element in the treatment of cancer, and can also act as neuroprotective, antioxidant, anti-inflammatory, antimicrobial, antitumoral, and antiparasitic among other assets [ 8 , 12 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ] as numerous studies have shown.…”

Section: Introductionmentioning

confidence: 99%

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Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

et al. 2023

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Tobacco cultivation and industrialization are characterized by the production of trillions of pre-harvest and post-harvest waste biomasses each year with the resulting negative effects on the environment. The leaves of blunt, pre-harvest waste, could be further used to obtain bioactive metabolites, i.e., polyphenols and alkaloids, for its potential cosmetic use. This study was conducted to obtain bio-compounds from pre-harvest tobacco leaf waste (var. Virginia) by applying conventional and green solvents (NaDES). Leaves and ground leaf waste were characterized based on their microscopic features. Conventional solvents, such as water, acetone, ethanol, and non-conventional solvents, such as Natural Deep Eutectic Solvents (NaDES), i.e., sucrose:lactic acid (LAS), frutose:glucose:sucrose (FGS), lactic acid:sucrose:water (SALA), choline chloride:urea (CU), and citric acid: propylene glycol (CAP) were used for bioactive extraction from tobacco waste powder. CU, FGS, and acetone/ethanol had similar behavior for the best extraction of alkaloids (6.37–11.23 mg ACE/g tobacco powder). LAS, FGS, SALA, and CU were more effective in phenolic compound extraction than conventional solvents (18.13–21.98 mg AGE/g tobacco powder). Because of this, LAS and SALA could be used to obtain phenolic-enriched extracts with lower alkaloid content rather than CU and FGS. Extracts of the powder obtained with conventional solvent or CU showed a high level of sugars (47 mg/g tobacco powder) The ABTS antioxidant capacity of tobacco leaf powder was higher in the extracts obtained with CU, FGS, and acetone (SC50 1.6–5 µg GAE/mL) while H2O2 scavenging activity was better in the extracts obtained with LAS, CAP and SALA (SC50 3.8–8.7 µg GAE/mL). Due to the biocompatibility of the NaDES with the components of tobacco leaf waste, the opportunity to apply these extracts directly in antioxidant formulations, such as cosmetics, phytotherapic, and other formulations of topic use seems promising. Furthermore, NaDES constituents, i.e., urea and organic acid can also have beneficial effects on the skin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

et al. 2023

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“…Nevertheless, the ability of these extracts to inhibit LPO could be attributed (at least in part) to the capacity of their chemical constituents to chelate/inactivate Fe 2+ , thereby, preventing or reducing reactive oxygen species generation. The HPLC fingerprint of these extracts revealed the presence of phenolic acids (Gallic, ellagic, chlorogenic and caffeic acids) and flavonoids (quercetin, quercitrin and rutin), compounds that are known scavengers and inhibitors of LPO [17,18]. Of particular importance, is the finding which revealed that chlorogenic and caffeic acids could inhibit free radicals formation through several mechanisms including the reaction complex formation with iron ions in the reactions of hydrogen peroxide with iron (II), hydrogen peroxide with ferric iron and 3-hydroxyanthranilic acid [19].…”

Section: Resultsmentioning

confidence: 99%

Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer

et al. 2015

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Raphiodon echinus (R. echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. echinus on human erythrocytes and leukocytes were investigated for the first time. R. echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. echinus exhibited antioxidant activity in DPPH radical scavenging with IC 50 = 111.9 µg/mL (EtOH extract) and IC 50 = 227.9 µg/mL (aqueous extract). The extracts inhibited Fe 2+ (10 µM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30-480 µg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.

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“…The enhanced membrane fragility index is further exacerbated by high oxidative stressed status of sickle erythrocytes [39][40][41]. Stabilization of erythrocyte membrane structures by herbal extracts is attributable to antioxidant activities and membrane chaotropic agent antagonists of phytocomponents from diverse plants [21,26,[42][43][44]. Because of limited scope of the previous investigations [21,26,43], the combinations of phytocomponents from the herbal extracts that stabilized the erythrocyte membrane against osmotic stress were not identified, quantified, and characterized.…”

Section: Introductionmentioning

confidence: 99%

Phytocomponents from Anacardium occidentale, Psidium guajava, and Terminalia catappa altered membrane osmotic stability of sickle erythrocytes

Chikezie

Ekeanyanwu

Chile-Agada

2020

Beni-Suef Univ J Basic Appl Sci

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Background: The present study identified, quantified, and characterized the combinations of phytocomponents from fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa that stabilized sickle erythrocyte membrane against osmotic stress, or otherwise, using standard chromatographicspectrophotometric techniques, namely GC-MS, FT-IR, and UV-visible systems. Results: The percentage hemolysis of the control sample, in 0.9 g/100 mL NaCl, was 35.08 ± 11.64%, whereas those of the samples containing 40 mg/100 mL, 60 mg/100 mL, and 80 mg/100 mL of ethylacetate extracts of T. catappa ranged between 31.82 ± 8.32 and 39.18 ± 6.94%. Ethylacetate extract of T. catappa contained comparative high quantities of hexadecanoic acid methyl ester, 9,11-octadecadienoic acid, methyl ester, (E, E)-, trans-13-octadecenoic acid methyl ester, and methyl stearate. FT-IR and UV-visible spectra showed that ethylacetate extract of T. catappa contained aromatic compounds as well as nitro-compounds, phenolics, and esters. Conclusion: To mention but a few, the combinations of major phytocomponents that stabilized sickle erythrocyte membrane against osmotic stress were hexadecanoic acid, methyl ester, 11-octadecenoic acid, methyl ester, dibutyl phthalate, pentacosane, trans-13-octadecenoic acid, methyl ester, whereas the minor phytocomponents include methyl tetradecanoate, methoxyacetic acid, 3-pentadecyl ester, methyl stearate, hexadecanoic acid, isoxazole, 4,5-dimethyl-.

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Membrane stability and methaemoglobin content of human erythrocytes incubated in aqueous leaf extract of Nicotiana tabacum product

Cited by 5 publications

References 46 publications

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer

Phytocomponents from Anacardium occidentale, Psidium guajava, and Terminalia catappa altered membrane osmotic stability of sickle erythrocytes

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