Membrane skeleton hyperstability due to a novel alternatively spliced 4.1R can account for ellipsoidal camelid red cells with decreased deformability

Chen, Yuqi; Miyazono, Kosuke; Otsuka, Yayoi; Kanamori, Mariko; Yamashita, Aozora; Arashiki, Nobuto; Matsumoto, Takehisa; Takada, Kouhei; Sato, Kota; Mohandas, Narla; Inaba, Mutsumi

doi:10.1016/j.jbc.2023.102877

Cited by 2 publications

(3 citation statements)

References 60 publications

(122 reference statements)

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“…As a mechanical property, RBC deformability depends on the cell membrane structure and composition, and their alteration should thereby correlate with changes in biochemical measures. Previous studies, both experimental and numerical simulations, linked the cell deformability to the content of specific membrane proteins, particularly stomatin, band-3, and protein 4.1R [ 37 , 38 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins

Barshtein

Gural²,

Arbell

et al. 2023

IJMS

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Red blood cell (RBC) deformability, expressing their ability to change their shape, allows them to minimize their resistance to flow and optimize oxygen delivery to the tissues. RBC with reduced deformability may lead to increased vascular resistance, capillary occlusion, and impaired perfusion and oxygen delivery. A reduction in deformability, as occurs during RBC physiological aging and under blood storage, is implicated in the pathophysiology of diverse conditions with circulatory disorders and anemias. The change in RBC deformability is associated with metabolic and structural alterations, mostly uncharacterized. To bridge this gap, we analyzed the membrane protein levels, using mass spectroscopy, of RBC with varying deformability determined by image analysis. In total, 752 membrane proteins were identified. However, deformability was positively correlated with the level of only fourteen proteins, with a highly significant inter-correlation between them. These proteins are involved in membrane rafting and/or the membrane–cytoskeleton linkage. These findings suggest that the reduction of deformability is a programmed (not arbitrary) process of remodeling and shedding of membrane fragments, possibly mirroring the formation of extracellular vesicles. The highly significant inter-correlation between the deformability-expressing proteins infers that the cell deformability can be assessed by determining the level of a few, possibly one, of them.

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Section: Discussionmentioning

confidence: 99%

Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins

Barshtein

Gural²,

Arbell

et al. 2023

IJMS

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show abstract

“…Regarding such variations in RBCs’ shape, size, and deformability, there is no unique description of optimized RBC characteristics. The evolutionary mechanism at the molecular level that makes the difference between ellipsoidal- and discoidal-shaped RBCs is still under investigation [5] .…”

Section: Introductionmentioning

confidence: 99%

“…The other major difference between camel and human RBCs is the marginal band (MB). It is a circumferential bundle, made of microtubules that Camelid RBCs have whereas human RBCs do not [5] , [23] , [24] . It is probable functions were thought of as altering and perhaps maintaining cell shape [25] .…”

Section: Introductionmentioning

confidence: 99%

Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy

Pesen,

Haydaroglu,

Capar

et al. 2023

Biochemistry and Biophysics Reports

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Membrane skeleton hyperstability due to a novel alternatively spliced 4.1R can account for ellipsoidal camelid red cells with decreased deformability

Cited by 2 publications

References 60 publications

Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins

Red Blood Cell Deformability Is Expressed by a Set of Interrelated Membrane Proteins

Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy

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