Membrane Skeleton Detachment in Spherical and Cylindrical Microexovesicles

Hägerstrand, Henry

doi:10.1006/bulm.1999.0128

Cited by 31 publications

(22 citation statements)

References 38 publications

(74 reference statements)

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“…51,52 These microvesicles are enriched with membrane proteins 53 and, their release might depends on the level of membrane fluidity. 54 In summary, the alterations observed in some membrane proteins indicate that the surfactants change lipid-protein interactions in the bilayer, which can be a biophysical mechanism directly related to membrane lysis.…”

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confidence: 99%

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Nogueira¹,

Mitjans

Busquets³

et al. 2012

Langmuir

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Amino acid-based surfactants constitute an important class of natural surface-active biomolecules with unpredictable number of industrial applications. To gain better mechanistic understanding of surfactant-induced membrane destabilization, we assessed the phospholipid bilayer-perturbing properties of new cationic lysine-based surfactants. We used erythrocytes as biomembrane models to study the hemolytic activity of surfactants and their effects on cells' osmotic resistance and morphology, as well as on membrane fluidity and membrane protein profile with varying pH. The antihemolytic capacity of amphiphiles correlated negatively with the length of the alkyl chain.Anisotropy measurements showed that the pH-sensitive surfactants, with the positive charge on the α-amino group of lysine, significantly increased membrane fluidity at acidic conditions. SDS-PAGE analysis revealed that surfactants induced significant degradation of membrane proteins in hypo-osmotic medium and at pH 5.4. By scanning electron microscopy examinations, we corroborated the interaction of surfactants with lipid bilayer. We found that varying the surfactant chemical structure is a way to modulate the positioning of the molecule inside bilayer and, thus, the overall effect on the membrane. Our work showed that pH-sensitive lysine-based surfactants significantly disturb the lipid bilayer of biomembranes especially at acidic conditions, which suggests that these compounds are promissing as a new class of multifunctional bioactive excipients for active intracellular drug delivery.

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confidence: 99%

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Nogueira¹,

Mitjans

Busquets³

et al. 2012

Langmuir

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“…Tubular microexovesicles were released by dodecyl D-maltoside (Fig. 6A) [22] or dimeric dioctyldiQAS [15], which are examples of strongly anisotropic inclusions. Further, in human erythrocytes polyoxyethyleneglycolalkylether induces a large oblate torocytelike endovesicle [17].…”

Section: Resultsmentioning

confidence: 99%

“…5), where spherical, oblate (flattened) and prolate (long tubular) shapes of the membrane were obtained. Tubular structures [22] could be linked to the calculated prolate ellipsoidal vesicle shape of the ellipsoid, while torocyte-like vesicles [17] could be linked to the calculated oblate ellipsoidal vesicle shape. Anisotropic inclusions with a large intrinsic curvature deviator (Fig.…”

Section: Resultsmentioning

confidence: 99%

Shape variation of bilayer membrane daughter vesicles induced by anisotropic membrane inclusions

Bohinc¹,

Lombardo

Kralj‐Iglič³

et al. 2006

Cellular and Molecular Biology Letters

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A theoretical model of a two-component bilayer membrane was used in order to describe the influence of anisotropic membrane inclusions on shapes of membrane daughter micro and nano vesicles. It was shown that for weakly anisotropic inclusions the stable vesicle shapes are only sligthly out-of-round. In contrast, for strongly anisotropic inclusions the stable vesicle shapes may significantly differ from spheres, i.e. they have a flattened oblate shape at small numbers of inclusions in the membrane, and an elongated cigar-like prolate shape at high numbers of inclusions in the vesicle membrane.

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“…The addition of anti-CTB reduces the number of membrane nanotubes, which might be due to the formation of CTB-GM1-anti-CTB protein network of nonzero shear elasticity that cannot be deformed into long tubes. 18 Since cell metabolism determines at least partially the growth of membrane nanotubes, a change in the temperature of the growth medium may affect the expression level of membrane nanotubes. A previous experimental study showed that temperature treatment could cause phase separation of GM1 molecules, changing the diameter of giant unilamellar vesicles (GUVs).…”

Section: Introductionmentioning

confidence: 99%

“…18,33 As a result, the bound network of CTB molecules at the membrane would prevent the formation of new membrane nanotubes as well as destabilize those already formed.…”

mentioning

confidence: 99%

Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

Kabaso¹,

Lokar²,

Kralj‐Iglič³

et al. 2011

IJN

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The growth of membrane nanotubes is crucial for intercellular communication in both normal development and pathological conditions. Therefore, identifying factors that influence their stability and formation are important for both basic research and in development of potential treatments of pathological states. Here we investigate the effect of cholera toxin B (CTB) and temperature on two pathological model systems: urothelial cell line RT4, as a model system of a benign tumor, and urothelial cell line T24, as a model system of a metastatic tumor. In particular, the number of intercellular membrane nanotubes (ICNs; ie, membrane nanotubes that bridge neighboring cells) was counted. In comparison with RT4 cells, we reveal a significantly higher number in the density of ICNs in T24 cells not derived from RT4 without treatments ( P = 0.005), after 20 minutes at room temperature ( P = 0.0007), and following CTB treatment ( P = 0.000025). The binding of CTB to GM1–lipid complexes in membrane exvaginations or tips of membrane nanotubes may reduce the positive spontaneous (intrinsic) curvature of GM1–lipid complexes, which may lead to lipid mediated attractive interactions between CTB–GM1–lipid complexes, their aggregation and consequent formation of enlarged spherical tips of nanotubes. The binding of CTB to GM1 molecules in the outer membrane leaflet of membrane exvaginations and tips of membrane nanotubes may also increase the area difference between the two leaflets and in this way facilitate the growth of membrane nanotubes.

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Membrane Skeleton Detachment in Spherical and Cylindrical Microexovesicles

Cited by 31 publications

References 38 publications

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Shape variation of bilayer membrane daughter vesicles induced by anisotropic membrane inclusions

Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

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