Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments

Lee, Chia‐Wei; Jang, Lan‐Ling; Pan, Huei-Jyuan; Chen, Yun‐Ru; Chen, Chih‐Cheng; Lee, Chau‐Hwang

doi:10.1186/s12951-016-0161-5

Cited by 22 publications

(16 citation statements)

References 26 publications

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“…4e, f) indicated that the roughness of the dehydrated CDs-fed cells was ∼49% higher than that of the pristine cells (104.3 ± 28.1 nm vs. 70.0 ± 22.1 nm, n = 24). According to the literature 19,20 , in addition to the attachment on the cell surface, the profile of intracellular particles or their clusters (with size detectable by AFM) can be reflected from the varied roughness of dehydrated cells, as the cells have lost the flexible and malleable cytoskeleton. To further explore whether the CDs were attached on the cell surface specifically or also being able to distribute inside the cells, cryo-electron tomography was employed.…”

Section: Resultsmentioning

confidence: 99%

Carbon dots-fed Shewanella oneidensis MR-1 for bioelectricity enhancement

et al. 2020

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Bioelectricity generation, by Shewanella oneidensis (S. oneidensis) MR-1, has become particularly alluring, thanks to its extraordinary prospects for energy production, pollution treatment, and biosynthesis. Attempts to improve its technological output by modification of S. oneidensis MR-1 remains complicated, expensive and inefficient. Herein, we report on the augmentation of S. oneidensis MR-1 with carbon dots (CDs). The CDs-fed cells show accelerated extracellular electron transfer and metabolic rate, with increased intracellular charge, higher adenosine triphosphate level, quicker substrate consumption and more abundant extracellular secretion. Meanwhile, the CDs promote cellular adhesion, electronegativity, and biofilm formation. In bioelectrical systems the CDs-fed cells increase the maximum current value, 7.34 fold, and power output, 6.46 fold. The enhancement efficacy is found to be strongly dependent on the surface charge of the CDs. This work demonstrates a simple, costeffective and efficient route to improve bioelectricity generation of S. oneidensis MR-1, holding promise in all relevant technologies.

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Section: Resultsmentioning

confidence: 99%

Carbon dots-fed Shewanella oneidensis MR-1 for bioelectricity enhancement

et al. 2020

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“…In other words, membrane roughness is modulated by way of actin filaments in cells responding to substrate properties. In our previous work, we showed that the membrane roughness of neuronal N2a cells was sensitive to the treatment of a microtubule-stabilization drug paclitaxel (Taxol) 12 . Here we also tested the effect of Taxol on the membrane roughness in MEFs and found that the Taxol treatment for 4 hours had no significant effect on the membrane roughness (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous works 3 , 12 , we demonstrated that the membrane roughness of neuroblastoma cells is sensitive to various external stimulations, including amyloid peptides of different conformations, electric fields, an anti-cancer drug Taxol, gold nanoparticles, and a hypertonic solution. Nonetheless, the treatments used in those previous works were all solution-based.…”

Section: Discussionmentioning

confidence: 97%

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Substrate properties modulate cell membrane roughness by way of actin filaments

Chang

Lee²,

Lee

2017

Sci Rep

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Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions. In order to know whether membrane roughness is associated with the substrate properties, we employed the non-interferometric wide-field optical profilometry (NIWOP) technique to measure the membrane roughness of living mouse embryonic fibroblasts with different conditions of the culture substrate. By controlling the surface density of fibronectin (FN) coated on the substrate, we found that cells exhibited higher membrane roughness as the FN density increased in company with larger focal adhesion (FA) sizes. The examination of membrane roughness was also confirmed with atomic force microscopy. Using reagents altering actin or microtubule cytoskeletons, we provided evidence that the dynamics of actin filaments rather than that of microtubules plays a crucial role for the regulation of membrane roughness. By changing the substrate rigidity, we further demonstrated that the cells seeded on compliant gels exhibited significantly lower membrane roughness and smaller FAs than the cells on rigid substrate. Taken together, our data suggest that the magnitude of membrane roughness is modulated by way of actin dynamics in cells responding to substrate properties.

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“…The plane fit more accurately revealed surface roughness. Recently, Lee et al [58] demonstrated that Taxol-induced decrease in the roughness of neuroblastoma cells indicates an increase of membrane stiffness caused by microtubule translocation. In addition, they showed a time-lapse measurement of the decrease in membrane tension induced by a hypertonic solution that resulted in an increase of membrane roughness.…”

Section: The New Biomarkers For Identifying the Neuroprotective Effecmentioning

confidence: 99%

Atomic force microscopy reveals new biomarkers for monitoring subcellular changes in oxidative injury: neuroprotective effects of quercetin at the nanoscale

Jembrek

Vlaini

ade

et al. 2018

Preprint

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Oxidative stress is a process involved in the pathogenesis of many diseases, including atherosclerosis, hypertension, diabetes, Alzheimer's disease etc. The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding cellular effects of free radicals and evaluation of the efficacy of drug treatment. Here, an interdisciplinary approach using atomic force microscopy (AFM) and cellular and biological molecular methods were used to obtain new potential biomarkers for monitoring oxidative stress condition. Biological methods confirmed the oxidative damage of investigated P19 neurons and revealed the underlying mechanism of quercetin protective action. AFM was employed to evaluate morphological (roughness) and nanomechanical (elasticity) properties that may be specific biomarkers for oxidative stress-induced cytoskeletal reorganization manifested by changes in the lateral dimension and height of neuronal somas.The morphological and nanomechanical analysis of neurons showed the strong mutual correlation between changes in cell membrane elasticity and neuroprotective effects of quercetin. Our findings indicate that AFM is a highly valuable tool for biomedical applications, detection and clarifying of drug-induced changes at the nanoscale and emphasize the potential of AFM approach in the development of novel therapeutic strategies directed against oxidative stress-induced neurodegeneration.

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Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments

Cited by 22 publications

References 26 publications

Carbon dots-fed Shewanella oneidensis MR-1 for bioelectricity enhancement

Carbon dots-fed Shewanella oneidensis MR-1 for bioelectricity enhancement

Substrate properties modulate cell membrane roughness by way of actin filaments

Atomic force microscopy reveals new biomarkers for monitoring subcellular changes in oxidative injury: neuroprotective effects of quercetin at the nanoscale

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