Membrane Protein Modified Electrodes in Bioelectrocatalysis

Zhang, Huijie; Catania, Rosa; Jeuken, Lars J. C.

doi:10.3390/catal10121427

Cited by 10 publications

(6 citation statements)

References 236 publications

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“…1c). Isolated PETCs come in many forms, including membrane discs, liposomes, structured lipid bilayers, or multi-lamellar membranes 1 , which can all be interfaced with electrodes, electron mediators and catalysts 45,46 . In ex vivo systems all liberated electrons can be redirected to artificial processes.…”

Section: In Vivo Vs Ex Vivo Systemsmentioning

confidence: 99%

Rewiring photosynthetic electron transport chains for solar energy conversion

Lawrence,

Egan,

Hoefer

et al. 2023

Nat Rev Bioeng

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Photosynthetic organisms have evolved versatile electron transport chains capable of the efficient conversion of solar energy into chemical energy. Researchers can engineer these electron transport pathways to drive new-to-nature processes in a class of systems we term "rewired photosynthetic electron transport chains" (R-PETCs). R-PETCs can be used for the light-driven production of electricity or chemicals and exhibit numerous advantages over the synthetic systems widely used for these applications, including enhanced stability, versatility, and sustainability. In this review we summarise the current state of R-PETC research, highlighting major advances alongside outstanding research problems. We also provide descriptions of R-PETC development, outlining the different classes of R-PETCs, the research tools used in their construction, and the key design considerations important for achieving high-performances. Finally, we identify future avenues for R-PETC research which we expect will enhance performances sufficiently to make these systems suitable for a variety of real-world applications.

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Section: In Vivo Vs Ex Vivo Systemsmentioning

confidence: 99%

Rewiring photosynthetic electron transport chains for solar energy conversion

Lawrence,

Egan,

Hoefer

et al. 2023

Nat Rev Bioeng

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“…Vesicles made of natural or synthetic lipids (liposomes) are a suitable platform for mimicking membrane structures and functions found in nature. , Liposomes have been widely exploited to fabricate artificial compartments in bottom-up synthetic biology (artificial cells and organelles) and nanoreactors in compartmentalized (photo)catalysis. , Functionalization of liposomes in biotechnology is achieved by the reconstitution of membrane proteins (MPs), which in spite of their complex amphiphilic nature, have an increasing number of promising applications in areas such as drug discovery, vaccines, biosensors, and energy conversion . However, the application of proteoliposomes is still hampered by the lack of chemical and physical long-term stability (typically days) and the complexity of purification and reconstitution of MPs. , …”

Section: Introductionmentioning

confidence: 99%

“…3,4 Functionalization of liposomes in biotechnology is achieved by the reconstitution of membrane proteins (MPs), which in spite of their complex amphiphilic nature, have an increasing number of promising applications in areas such as drug discovery, 5 vaccines, 6 biosensors, 7 and energy conversion. 8 However, the application of proteoliposomes is still hampered by the lack of chemical and physical long-term stability (typically days) 9 and the complexity of purification and reconstitution of MPs. 10,11 Recent developments using amphiphilic polymers have shown promise in solving these experimental limitations.…”

Section: ■ Introductionmentioning

confidence: 99%

Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs

et al. 2022

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Hybrid vesicles (HVs) that consist of mixtures of block copolymers and lipids are robust biomimetics of liposomes, providing a valuable building block in bionanotechnology, catalysis, and synthetic biology. However, functionalization of HVs with membrane proteins remains laborious and expensive, creating a significant current challenge in the field. Here, using a new approach of extraction with styrene-maleic acid (SMA), we show that a membrane protein (cytochrome bo 3 ) directly transfers into HVs with an efficiency of 73.9 ± 13.5% without the requirement of detergent, long incubation times, or mechanical disruption. Direct transfer of membrane proteins using this approach was not possible into liposomes, suggesting that HVs are more amenable than liposomes to membrane protein incorporation from a SMA lipid particle system. Finally, we show that this transfer method is not limited to cytochrome bo 3 and can also be performed with complex membrane protein mixtures.

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“…Membrane proteins, constituting 20-30% of all proteins secreted by living organisms, are a major subject of bioelectrocatalysis. In the review by Zhang et al, various and eventually coupled techniques, i.e., electrochemistry, spectroscopy, microscopy, and quartz crystal microbalance [7] are discussed toward the understanding and use of membrane enzymes active in bioenergy conversion. Electrode designs with a special focus on the specificity required for membrane proteins are highlighted.…”

mentioning

confidence: 99%