Membrane processes application on the Symphytum officinale and Geranium robertianum extracts concentration to obtain high antioxidative activity compounds

Abstract

The paper reports the successful application of membranes processes to obtain the good quality extracts with compounds of high antioxidative activity and therapeutic value. This study provided investigations on the phenolic compounds from two plant species used in Romanian ethno-medicine and evaluated the antioxidant and cytotoxic activities. Three extracts prepared from Geranium robertianum and Symphytum officinale were concentrated by microfiltration and ultrafiltration. The levels of phenolic compounds and flavonoids were determined by UV-Vis spectroscopy and HPLC. The freeradical scavenging activity of concentrated extracts was determined by DPPH method. The preliminary tests of cytotoxic activity for the concentrated extracts have been carried out on human epidermoid laryngeal carcinoma cell line (Hep-2p) and normal monkey kidneys cells (RM). The results showed that all concentrated extracts had a very low cytotoxicity against healthy cells, but a significant cytotoxic effect on Hep-2p tumor cells. The concentrated extracts have a high antioxidant activity (% DPPH inhibition > 80%)

Chemical characterization and bioactive properties of aqueous and organic extracts of Geranium robertianum L.

“…Only two recent reports on the antitumor potential of aqueous and aqueous/ethanolic extracts of plants native from Romania against human epidermoid laryngeal carcinoma cells (Hep-2p) can be found in the literature. 7,9 Phytochemical studies of this species have been mainly focused on phenolic compounds from alcoholic or hydroalcoholic extracts, more particularly flavonoids. [7][8][9][10]13,[18][19][20][21] The chemical composition of its essential oils has received much less attention.…”

Phytochemical composition and biological activities of Geranium robertianum L.: A review

“…robertianum contains other phenolic acids also resulting from the shikimate biosynthetic pathway such as ferulic acid (6a) and its precursor, the caffeic acid (7) (Bate-Smith, 1962;Fodorea et al, 2005;Kobakhidze and Alaniya, 2004;Paun et al, 2011;Paun et al, 2012). Ferulic acid was additionally detected as methyl (6b) and ethyl (6c) esters (Amaral et al, 2009).…”

“…Flavonoids constitute the main compounds found in G. robertianum (Table 2) and can be found in relatively high amounts (Igwenyi and Elekwa, 2014;Ben Jemia et al 2013;Neagu et al, 2010a;Paun et al, 2011;Paun et al, 2012;Paun et al, 2014). Within this class of secondary metabolites, flavonols, namely, quercetin and kaempferol, are predominant.…”

“…The foliage of specimens from England was referred to contain high concentrations of Ca, Na and Fe (Grime et al, 1988). Aqueous and hydro-alcoholic extracts of plants native from Romania were also screened for dietary elements and Ca, Mg, Mg, Zn and Fe were found to be present in reasonable amounts (Table 3) (Paun et al, 2012).…”

Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity

“…robertianum contains many biologically active compounds, mostly polyphenols (Amaral et al, 2009;Fodorea et al, 2005;Ivancheva and Petrova, 2000;Kartnig and Bucar-Stachel, 1991;Kobakhidze and Alaniya, 2004;Neagu et al, 2013;Paun et al, 2011Paun et al, , 2012Paun et al, , 2014.…”