Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity

Graça, Vânia; Barros, Lillian; Calhelha, Ricardo C.; Días, María Inês; Ferreira, Isabel C.F.R.; Santos, Paulo F.

doi:10.1016/j.indcrop.2017.07.016

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…Fractionation was carried out in the extracts that showed promising results in the bioactivity assays, namely DCM and n-hexane extracts. The procedure was conducted based on the methodology described by Graça et al [28], with some modifications.…”

Section: Fractionation Of the Extractsmentioning

confidence: 99%

Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions

et al. 2020

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Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.

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Section: Fractionation Of the Extractsmentioning

confidence: 99%

Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions

et al. 2020

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“…Compounds 1–9 were previously reported in a study on P. graveolens [ 55 ]. Compounds 2, 6, and 7 have been reported in other species of the Geraniacae family, except from geranium, i.e., Geranium molle L. [ 56 ] and Geranium robertianum L. [ 57 ]. Finally, to the best of our knowledge compounds 10, 11 have not been reported in Geraniacae family.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Essential Oils and Extracts of Rose Geranium and Rose Petals as Natural Preservatives in Terms of Toxicity, Antimicrobial, and Antiviral Activity

Androutsopoulou

Christopoulou

Hahalis³

et al. 2021

Pathogens

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Essential oils (EOs) and extracts of rose geranium (Pelargonium graveolens) and petals of rose (Rosa damascena) have been fully characterized in terms of composition, safety, antimicrobial, and antiviral properties. They were analyzed against Escherichia coli, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Aspergillus niger, and Adenovirus 35. Their toxicity and life span were also determined. EO of P. graveolens (5%) did not retain any antibacterial activity (whereas at 100% it was greatly effective against E. coli), had antifungal activity against A. niger, and significant antiviral activity. Rose geranium extract (dilutions 25−90%) (v/v) had antifungal and antibacterial activity, especially against E. coli, and dose-dependent antiviral activity. Rose petals EO (5%) retains low inhibitory activity against S. aureus and S. Typhimurium growth (about 20−30%), antifungal activity, and antiviral activity for medium to low virus concentrations. Rose petals extract had significant antibacterial activity at dilutions of 25−90%, especially against E. coli and S. Typhimurium, antifungal, and the most potent antiviral activity. None of the EOs and extracts were toxic in dilutions of up to 5% and 90%, respectively. Finally, all materials had a life span of more than eight weeks. These results support the aspect that rose petals and rose geranium EOs, and extracts, have beneficial antimicrobial and antiviral properties and they can be used as natural preservatives.

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“…Medicinal plants have been used as an alternative to orthodox medicine in many countries, Nigeria inclusive. Despite their widespread use, few scientific studies have been undertaken to ascertain the efficacy of traditional remedies (Graça et al, 2017). Thousands of plant species have been identified as a good source of therapeutics, and the use of these compounds is gradually increasing (Ishii et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory Activity of Aqueous Leaves Extract of Cassia Occidentalis on Innate and Adaptive Immune Response in Mice

Hamid¹,

Kalgo²,

Isyaku³

et al. 2021

FJS

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Different types of plants have been studied for their roles in immunostimulation and treatment of other diseases. The study aimed at evaluating the stimulatory activity of aqueous leaf extract of Cassia occidentalis on innate and adaptive immune responses in mice. Study mice were grouped into five and studied for 21 days; group I (control group) received normal saline. While the treatment groups: group II - V received aqueous leaf extract of C. occidentalis at different concentrations (250, 500, 1000, and 1500) mg/kg body weight (BW). The mice were sensitized and challenged with sheep red blood cells (SRBCs) on day 13th and 18th of the treatment, respectively. On the 19th, 20th, and 21st days, the animals’ delayed-type hypersensitivity (DTH) responses in each group were measured using a digital Vernier calliper, and humoral immune response was evaluated using the haemagglutination (HA) antibody technique. Blood samples were collected for Carbonic particle clearance index (K) and phagocytic index of Macrophage (α). Organ weight index (g/100g) was also recorded (α). The result showed a significant increase in the Carbonic particle clearance index (p=0.02) and phagocytic index of Macrophage (p<0.0001) in a dose-dependent manner. The study revealed a statistically significant difference in HA titres across the five groups, (p=0.02), also the DTH shows a statistically significant difference across the time (24 hours, 48 hours, 72 hours), Wilks’ Lambda = 0. 291, F = 15. 86, p = 0. 0001. The aqueous leaf extract of C. occidentalis has an immunostimulatory effect on innate and adaptive immunity.

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Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity

Cited by 10 publications

References 37 publications

Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions

Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions

Evaluation of Essential Oils and Extracts of Rose Geranium and Rose Petals as Natural Preservatives in Terms of Toxicity, Antimicrobial, and Antiviral Activity

Immunostimulatory Activity of Aqueous Leaves Extract of Cassia Occidentalis on Innate and Adaptive Immune Response in Mice

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