Membrane potentials of thin nitrocellulose membranes

Carnell, Paul H.; McGunegle, Daniel E.

doi:10.1002/app.1967.070110606

Cited by 1 publication

(1 citation statement)

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“…Employing nitrocellulose in diagnostic kits is important for two reasons. First, nitrocellulose fibers possess high protein absorption ability and second, their absorption ability will not diminish in contact with aqueous solutions (Carnell & McGunegle, 1967;Holstein, Chevalier, Bennett, & Anderson, 2015;Lu, Shi, Qin, & Lin, 2010). The high capacity of nitrocellulose in the absorption of protein is due to the dipole interactions between the functioning nitro groups in the nitrocellulose and peptide bonds in the carbonyl group of proteins.…”

Section: Fixation Of Agglutinated Blood On Nitrocellulose Membranementioning

confidence: 99%

A novel method for blood‐typing using nitrocellulose

Afshari

Abolfathi

2017

Biomedical Chromatography

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Blood wicking in its steady-state form, i.e. the uniform distribution of blood cells in plasma, is completely different from that in its coagulated form on a porous surface like paper. The hydrophilic property of the cellulose leads to a significant wicking of the blood cells on paper fibers after rinsing with isotonic solution. The difference in the wicking length of the blood cells in steady state and that in the coagulated form could be considered as a criterion to recognize the blood type in a paper-based kit. However, owing to the molecular structure of the nitrocellulose, a better process occurs while separating the coagulated blood from the steady-state form of cells. Therefore, it is possible to use the nitrocellulose for the blood-typing kit which leads to a simpler way to diagnose a blood type. Two series of experiments were performed on nitrocellulose membrane. First, antibody solutions and blood samples were sequentially absorbed on nitrocellulose strips, allowed to interact, rinsed with an isotonic solution and distilled water, and image processing performed on a digital picture of the remaining blood cells. The efficiency of the agglutinated blood cell fixation was quantified by red color intensity. Then, it was demonstrated that there is no considerable difference in fixation of agglutinated blood cells with rinsing using isotonic and nonisotonic solutions. This fact can be a considerable advantage over paper since it can eliminate the probable mistake from using unisotonic solution for rinsing. Second, owing to the nonwicking property of the blood cells on the hydrophobic nitrocellulose fibers, we employed another diagnostic criterion and investigated nitrocellulose blood-typing prototypes. The nitrocellulose blood-typing kit provides more simple, sensitive and trustworthy assay for rapid blood typing in situations with no access to laboratory facilities.

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Section: Fixation Of Agglutinated Blood On Nitrocellulose Membranementioning

confidence: 99%