Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics

El-Nakeeb, Moustafa A.; Abou-Shleib, Hamida; Khalil, Amal M; Omar, Hoda; El-Halfawy, Omar M.

doi:10.1590/s1517-83822011000300019

Cited by 23 publications

(20 citation statements)

References 3 publications

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“…Alteration of membrane permeability is one of the major mechanisms underlying the drug tolerance phenomenon, and damage to the cell wall should affect overall cell permeability (28). We found that the cell membrane became permeable to a membrane-impermeant dye, PI (29), when cells were exposed to CUR along with CWP agents at synergistic concentrations.…”

Section: Discussionmentioning

confidence: 86%

Curcumin Targets Cell Wall Integrity via Calcineurin-Mediated Signaling in Candida albicans

Kumar

Dhamgaye

Maurya

et al. 2014

Antimicrob Agents Chemother

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Curcumin (CUR) shows antifungal activity against a range of pathogenic fungi, including Candida albicans. The reported mechanisms of action of CUR include reactive oxygen species (ROS) generation, defects in the ergosterol biosynthesis pathway, decrease in hyphal development, and modulation of multidrug efflux pumps. Reportedly, each of these pathways is independently linked to the cell wall machinery in C. albicans, but surprisingly, CUR has not been previously implicated in cell wall damage. In the present study, we performed transcriptional profiling to identify the yet-unidentified targets of CUR in C. albicans. We found that, among 348 CUR-affected genes, 51 were upregulated and 297 were downregulated. Interestingly, most of the cell wall integrity pathway genes were downregulated. The possibility of the cell wall playing a critical role in the mechanism of CUR required further validation; therefore, we performed specific experiments to establish if there was any link between the two. The fractional inhibitory concentration index values of 0.24 to 0.37 show that CUR interacts synergistically with cell wall-perturbing (CWP) agents (caspofungin, calcofluor white, Congo red, and SDS). Furthermore, we could observe cell wall damage and membrane permeabilization by CUR alone, as well as synergistically with CWP agents. We also found hypersusceptibility in calcineurin and mitogen-activated protein (MAP) kinase pathway mutants against CUR, which confirmed that CUR also targets cell wall biosynthesis in C. albicans. Together, these data provide strong evidence that CUR disrupts cell wall integrity in C. albicans. This new information on the mechanistic action of CUR could be employed in improving treatment strategies and in combinatorial drug therapy.

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Section: Discussionmentioning

confidence: 86%

Curcumin Targets Cell Wall Integrity via Calcineurin-Mediated Signaling in Candida albicans

Kumar

Dhamgaye

Maurya

et al. 2014

Antimicrob Agents Chemother

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“…Azelastine has been previously shown to induce alterations in the bacterial membrane permeability (6). This was demonstrated by induction of leakage of bacterial intracellular materials, damage to bacterial membranes as shown by TEM, and damage to negatively charged cholesterol-free unilamellar liposomes, an artificial membrane model.…”

Section: Resultsmentioning

confidence: 97%

“…Azelastine is a phthalazinone non-sedating new generation antihistaminic drug widely used in the management of allergic conditions. Azelastine possesses certain antibacterial activity against a variety of microorganisms (5) due to its ability to induce alterations in membrane permeability and disruption of its integrity as previously shown (6). However, its interaction with antibiotics was not reported in the literature.…”

mentioning

confidence: 95%

Reversal of antibiotic resistance in Gram‐positive bacteria by the antihistaminic azelastine

El-Nakeeb

Abou-Shleib

Khalil

et al. 2011

APMIS

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Antibiotic resistance represents a serious problem that complicates microbial infection. The use of 'helper compounds' capable of enhancing the antimicrobial activity of antibiotics is being investigated. Azelastine, a new generation antihistaminic, possesses certain antibacterial activity and is capable of inducing alteration in the bacterial membrane permeability. Hence, we hypothesized that it could reverse resistance to antibiotics. Azelastine significantly increased the antibacterial activity of eight antibiotics belonging to five different classes (β-lactams, macrolides, fluoroquinolones, aminoglycosides and tetracyclines) against nine Gram-positive clinical isolates: five Staphylococcus aureus, two Staphylococcus epidermidis and two Enterococcus faecium, seven of which were multi-drug resistant, reversing their resistance to the tested antibiotics. The synergistic effects of azelastine with the studied antibiotics increased with raising the pH from 5 to 8. Antibiotics did not affect the ability of azelastine to alter the permeability of a liposomal artificial membrane model, an effect thought to be critical for the interaction with antibiotics. The findings of this study present azelastine as a potential 'helper compound' that could reverse the resistance of multi-drug resistant Gram-positive clinical isolates to antibiotics.

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“…11 Saponins work by damaging the cytoplasmic membrane by reducing surface tension resulting in increased cell permeability and leakage resulting in intracellular compounds coming out. 12 The mechanism of action of phenolic as an antibacterial is poisoning protoplasm, damaging and penetrating walls and depositing bacterial cell proteins. Large molecular phenolic compounds are capable of activating essential enzymes in bacterial cells even in very low concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of action of the tannin as an antibacterial is to inhibit the reverse transcriptase and DNA topoisomerase enzymes so that the DNA transcription process is disrupted and new bacterial cells cannot be formed. 12…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566

2018

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Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

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Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics

Cited by 23 publications

References 3 publications

Curcumin Targets Cell Wall Integrity via Calcineurin-Mediated Signaling in Candida albicans

Curcumin Targets Cell Wall Integrity via Calcineurin-Mediated Signaling in Candida albicans

Reversal of antibiotic resistance in Gram‐positive bacteria by the antihistaminic azelastine

Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566

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