Membrane‐mediated capillary electrophoresis: Interaction of cationic peptides with bicelles

Mills, John O.; Holland, Lisa

doi:10.1002/elps.200405879

Cited by 29 publications

(30 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These model membranes have also shown a promising application in micellar electrokinetic chromatography as they mimic physiologically relevant conditions. More specifically, the bilayered structure allowed the separation of six beta blockers that were not soluble in micelles (125), and the membrane affinity of peptides to membranes was also studied using this technique (126). Bicelles are also useful in circular dichroism (CD) studies of membrane peptides, which complement well the structural data obtained by NMR.…”

Section: Resultsmentioning

confidence: 98%

Bicelles as model membranes for solid‐ and solution‐state NMR studies of membrane peptides and proteins

Marcotte

Auger

2005

Concepts Magnetic Resonance

190

196

View full text Add to dashboard Cite

ABSTRACT:Bicelles are an attractive membrane mimetic system because of their planar surface and lipid composition, which resemble biological membranes. In addition, their orientation and morphologic properties make them amenable to solid-and solution-state NMR. This article reviews the physical properties of bicelles, such as magnetic alignment and viscosity as well as the different models proposed in the literature to explain the bicelle morphology. The utility of bicelles for studying the interaction and structure of membrane peptides and proteins by solid-and solution-state NMR is also presented, along with the advantages and limitations of bicelles.

show abstract

Section: Resultsmentioning

confidence: 98%

Bicelles as model membranes for solid‐ and solution‐state NMR studies of membrane peptides and proteins

Marcotte

Auger

2005

Concepts Magnetic Resonance

190

196

View full text Add to dashboard Cite

show abstract

“…The labeled glycans are separated under reversed polarity by hydrodynamic volume with a phospholipid coating and a phospholipid additive. Previous reports have demonstrated a variety of applications based on phospholipid additives for Electrophoresis 2011, 32, 3491-3498 capillary electrophoresis [53][54][55], and have characterized the effects of temperature, phospholipid composition and concentration, injection protocol, separation field strength, and capillary length [50,51]. This paper demonstrates an effective strategy for sequence identification by sequentially incorporating enzymes within the separation capillary.…”

Section: Introductionmentioning

confidence: 95%

Online enzymatic sequencing of glycans from Trastuzumab by phospholipid‐assisted capillary electrophoresis

2011

Self Cite

View full text Add to dashboard Cite

CE separations of glycans taken from the cancer drug, Trastuzumab (Herceptin(®)), were accomplished using phospholipid additives. Glycans were labeled with 1-aminopyrene-3,6,8-trisulfonic acid and were separated with efficiencies as high as 510000 theoretical plates in a 60.2 cm 25 μm id fused-silica capillary. The thermally tunable phospholipid was loaded into the capillary when it possessed a viscosity similar to that of water. The temperature was increased, and the separations were performed when the material exhibited higher viscosity. Enzymes were integrated into the separation with the phospholipid additive. Neuraminidase, β1-4 galactosidase, and β-N-acetylglucosaminidase were injected into the capillary without covalent modification and used for enzyme hydrolysis. Exoglycosidase enzymes cleaved the terminal glycan residues. The glycan sequence could be verified based on enzyme specificity. Neuraminidase was used to determine total glycan content of the low-abundance glycans containing sialic acid. β1-4 Galactosidase and β-N-acetylglucosaminidase were used sequentially in-capillary, to determine the structure of the high-abundance glycans.

show abstract

“…Soon after its introduction, MEKC's potential for the separation of achiral ionic species with very similar electrophoretic mobilities as well as neutral and charged enantiomers was also recognized [5][6][7][8][9][10][11][12][13][14][15][16]. In parallel with the rapidly broadening areas of successful applications (e.g., pharmaceutical compounds [17][18][19][20][21], derivatized amino acids [22][23][24], water-and fat-soluble vitamins [25][26][27][28], and herbicides [29]) was the investigation of a wide variety of alternative PSPs including modified SDS micelles [30,31], non-SDS conventional micelles [2,32], mixed micelles [33,34], charged CDs [35], microemulsions [36], macrocyclic compounds [37], polymer micelles [38,39], bicelles [40,41], linear polymers [16], dendrimers [42], and pseudostationary ion-exchange phases [16].…”

Section: Introductionmentioning

confidence: 99%

Compositional effects on electrophoretic and chromatographic figures of merit in electrokinetic chromatography with cetyltrimethylammonium bromide/sodium octyl sulfate vesicles as the pseudostationary phase. Part 1: Effect of the phase ratio

et al. 2008

View full text Add to dashboard Cite

The effect of the phase ratio on the electrophoretic and chromatographic properties of unilamellar vesicles comprised of cetyltrimethylammonium bromide (CTAB) and sodium octyl sulfate (SOS) was investigated in EKC. The surfactant concentration of the vesicles was 0.9, 1.2, 1.5, and 1.8% w/v, with a mole ratio of 1:3.66 (CTAB/SOS). Results were compared to those obtained using SDS micelles at concentrations of 1.0% (w/v, 35 mM) and 1.5% (52 mM). The CTAB/SOS vesicles (0.9-1.8% w/v) provided a significantly larger elution range (5.7 < or = t(ves)/t(0) < or = 8.7) and greater hydrophobic (methylene) selectivity (2.8 < or = alpha(CH2) < or = 3.1) than SDS micelles (3.1 < or = t(mc)/t(0) < or = 3.3; alpha(CH2) = 2.2). Whereas the larger elution range can be attributed to the 25% reduction in EOF due to the interaction of unaggregated CTAB cations and the negatively charged capillary wall, the higher methylene selectivity is likely due to the lower concentration of water expected in the CTAB/SOS vesicle bilayer compared to the Palisades layer of SDS micelles. For a given phase ratio, CTAB/SOS vesicles are somewhat less retentive than SDS micelles, although retention factors comparable to those observed in 1.0-1.5% SDS can be obtained with 1.5-1.8% CTAB/SOS. A linear relationship was observed between phase ratio and retention factor, confirming the validity of the phase ratio model for these vesicles. Unique polar group selectivities and positional isomer shape selectivities were obtained with CTAB/SOS vesicles, with both types of selectivities being nearly independent of the phase ratio. For four sets of positional isomers, the elution order was always para < ortho < meta. Finally, the thermodynamics of solute retention was qualitatively similar to that reported for other surfactant aggregates (micelles and microemulsions); the enthalpic contribution to retention was consistently favorable for all compounds, whereas the entropic contribution was favorable only to hydrophobic solutes.

show abstract

Membrane‐mediated capillary electrophoresis: Interaction of cationic peptides with bicelles

Cited by 29 publications

References 23 publications

Bicelles as model membranes for solid‐ and solution‐state NMR studies of membrane peptides and proteins

Bicelles as model membranes for solid‐ and solution‐state NMR studies of membrane peptides and proteins

Online enzymatic sequencing of glycans from Trastuzumab by phospholipid‐assisted capillary electrophoresis

Compositional effects on electrophoretic and chromatographic figures of merit in electrokinetic chromatography with cetyltrimethylammonium bromide/sodium octyl sulfate vesicles as the pseudostationary phase. Part 1: Effect of the phase ratio

Contact Info

Product

Resources

About