Membrane lipid compositions and their difference between subcellular structures

Sim, Pei Fang; Noor, Wan Nurul Izzati Wan Mohamad; Inaba, Takehiko; Suetsugu, Shiro

doi:10.1016/b978-0-323-89911-6.00008-x

Cited by 3 publications

(5 citation statements)

References 115 publications

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“…This hypothesis is supported by two of the five top terms in the enrichment analyses, “above average bilayer thickness” and “high bilayer thickness.” Out of the 147 dysregulated lipids, 60.5% (89/147) were phospholipids, which are primarily located within cellular membranes (plasma membrane and organelle membranes). Although the lipid composition of each cellular membrane has a different abundance in lipid species, 129 we did not explore which cellular membrane (plasma membrane, endoplasmic reticulum, Golgi, etc.) may be most affected by TBT exposure.…”

Section: Discussionmentioning

confidence: 99%

“…Last, the term “negative intrinsic curvature” indicates that TBT may induce a concave membrane structure, which is driven by higher abundance of phosphatidylethanolamines and phosphatidic acid. 129 We hypothesize that the combined reduction in phosphatidylcholines, phosphatidylserines (drivers of no membrane curvature), and phosphatidylethanolamines results in membrane curvature changes. The direct impact of curvature changes upon TBT exposure on cell function, however, remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin

Pascuali,

Pu,

Waye

et al. 2024

Environ Health Perspect

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Background: Exposure to obesogenic chemicals has been reported to result in enhanced adipogenesis, higher adipose tissue accumulation, and reduced ovarian hormonal synthesis and follicular function. We have reported that organotins [tributyltin (TBT) and triphenyltin (TPT)] dysregulate cholesterol trafficking in ovarian theca cells, but, whether organotins also exert lipogenic effects on ovarian cells remains unexplored. Objective: We investigated if environmentally relevant exposures to organotins [TBT, TPT, or dibutyltin (DBT)] induce lipid dysregulation in ovarian theca cells and the role of the liver X receptor (LXR) in this effect. We also tested the effect of TBT on oocyte maturation and neutral lipid accumulation, and lipid-related transcript expression in cumulus cells and preimplantation embryos. Methods: Primary theca cell cultures derived from human and ovine ovaries were exposed to TBT, TPT, or DBT (1, 10, or ). The effect of these chemical exposures on neutral lipid accumulation, lipid abundance and composition, lipid homeostasis-related gene expression, and cytokine secretion was evaluated using liquid chromatography–mass spectrometry (LC-MS), inhibitor-based methods, cytokine secretion, and lipid ontology analyses. We also exposed murine cumulus-oocyte complexes to TBT and evaluated oocyte maturation, embryo development, and lipid homeostasis-related mRNA expression in cumulus cells and blastocysts. Results: Exposure to TBT resulted in higher intracellular neutral lipids in human and ovine primary theca cells. In ovine theca cells, this effect was dose-dependent, independent of cell stage, and partially mediated by LXR. DBT and TPT resulted in higher intracellular neutral lipids but to a lesser extent in comparison with TBT. More than 140 lipids and 9 cytokines were dysregulated in TBT-exposed human theca cells. Expression of genes involved in lipogenesis and fatty acid synthesis were higher in theca cells, as well as in cumulus cells and blastocysts exposed to TBT. However, TBT did not impact the rates of oocyte maturation or blastocyst development. Discussion: TBT induced dyslipidemia in primary human and ovine theca cells, which may be responsible for some of the TBT-induced fertility dysregulations reported in rodent models of TBT exposure. https://doi.org/10.1289/EHP13955

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin

Pascuali,

Pu,

Waye

et al. 2024

Environ Health Perspect

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“…PG is transported to the inner mitochondrial membrane and acts as a precursor for cardiolipin biosynthesis, a lipid found exclusively within mitochondria. PG is a relatively rare lipid species in mammalian membranes and is more abundant in bacterial membranes (Sim et al, 2023). Vancomycin may therefore localise to mitochondria in mammalian cells via the increased abundance of PG used for cardiolipin synthesis, however we also saw significant labelling of other membrane structures including the plasma membrane and nucleus.…”

Section: Discussionmentioning

confidence: 99%

“…The composition of membranes differs between intracellular organelles (Sim et al, 2023), and these differences may affect vancomycin binding in different cell types, but this may also be influenced by additional factors such as shape, charge and presence of transmembrane proteins. The function and integrity of mitochondria depends on the composition of lipids that make up their membranes.…”

Section: Discussionmentioning

confidence: 99%

Vancomycin impairs macrophage fungal killing by disrupting mitochondrial morphology and function

Bojang,

Sheriff,

et al. 2024

Preprint

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Vancomycin is a widely prescribed antibiotic used in the treatment of Gram-positive bacterial infections. We recently showed that this antibiotic disrupted protective anti-fungal immune responses via microbiome dysbiosis, enhancing susceptibility to invasive candidiasis. Antibiotics are an independent risk factor for developing this life-threatening fungal infection, but whether microbiota-independent mechanisms also drive this association is not clear. Here, we show that vancomycin directly impairs macrophage responses toCandida albicans, the main causative agent of invasive candidiasis. Vancomycin-treated macrophages were less able to killC. albicansdespite normal phagocytosis rates and were hyper-inflammatory and more likely to die during infection. We found that vancomycin bound to macrophage mitochondria, leading to depolarisation, reduced respiratory capacity and a hyper-fragmented morphology associated with increased ROS production. Taken together, this work demonstrates direct effects of vancomycin on mammalian immune cells, helping us to understand pro-inflammatory effects of this drug and how it promotes susceptibility to life-threatening fungal infection.

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“…Mitotracker barviva jsou skupinou fluorescenčních sond používaných v buněčné biologii k značení a sledování mitochondrií v živých buňkách, přičemž některá z nich jsou stabilní i po jejich fixaci 36 . Tato barviva jsou užitečná ke studiu morfologie, dynamiky a funkce mitochondrií prostřednictvím různých zobrazovacích technik, jako je fluorescenční mikroskopie a průtoková cytometrie 37 . Mitotracker barviva jsou navržena tak, aby se selektivně akumulovala v aktivních mitochondriích.…”

Section: Fluorescenční Sondy Používané Při Fenotypovém Testováníunclassified

Identification of Cellular Targets of Active Substances Using Microscopic Methods and Fluorescent Probes

Stanková,

Hajdúch,

Džubák

2024

Chem. Listy

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Target identification of active substances is critical in optimizing drugs and minimizing side effects. The complex nature of biological systems presents challenges; to meet them, however, microscopic methods, particularly phenotypic screening, represented by "Cell Painting" method and fluorescent probes, can be used as valuable tools for understanding the impact of various substances at the cellular and organelle levels. These methods enable rapid testing of large libraries of compounds and offer unique insights into their mechanism of action by observing cell behavior, assessing cell morphology, motility, division, and migration. However, live cell microscopy faces challenges like phototoxicity, requiring a careful selection of fluorescent labels and optimized conditions. Among synthetic probes for live cell microscopy, BODIPY dyes stand out for their synthetic versatility and photophysical properties, providing minimal sample damage during bioimaging.

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Membrane lipid compositions and their difference between subcellular structures

Cited by 3 publications

References 115 publications

Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin

Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin

Vancomycin impairs macrophage fungal killing by disrupting mitochondrial morphology and function

Identification of Cellular Targets of Active Substances Using Microscopic Methods and Fluorescent Probes

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