Membrane lipid changes in mouse blastocysts induced by ovarian stimulation, IVF and oocyte vitrification

Berteli, Thalita S.; Borges, Eduardo D.; Luz, Caroline Mantovani da; Vireque, A.A.; Navarro, Paula Andrea

doi:10.1016/j.rbmo.2023.01.007

Cited by 4 publications

(7 citation statements)

References 58 publications

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“…Membrane lipids, including PC, PE and PI lipids, showed higher relative intensities when lipid extraction was done using only with pure methanol, even though the same was not observed for PG and PS lipids. In this way, one-step lipid extraction with methanol for analysis of membrane lipids in oocytes and embryos has been reported successfully and allowed accurate identification of lipid changes within individual lipid classes [ 22 , 23 ]. Neutral lipids such as acyl-carnitines, cholesteryl esters and FFA were more efficiently extracted when a chloroform: methanol mixture was used.…”

Section: Discussionmentioning

confidence: 99%

Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling

de Lima,

Milazzotto,

Vireque

et al. 2023

Preprint

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Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic development. Lipid profiling in samples of minute size, such as oocytes, is challenging but has been achieved by mass spectrometry technologies such as multiple reaction monitoring (MRM) profiling. With the goals of further simplifying sample workflow and investigating the influence of pre-analytical conditions, we have evaluated how different extraction methods and transportation of lipid extracts in vacuum and at room temperature impacted the lipid profile of bovine oocytes. Using a comprehensive method, 316 MRMs associated with lipids of 10 different classes were screened in oocyte lipid extracts prepared by 2 extraction methods (one-step methanol addition or Bligh and Dyer) and transporting them in dry ice or at room temperature inside vacuum packages. No changes in the multivariate analysis (PCA) were noticeable due to transportation temperature, while lipid profiles were more affected by the lipid extraction protocol. Sample extraction using pure methanol favored the detection of phospholipids uniformly, while Bligh and Dyer favored the detection of neutral intracellular lipids. Triacylglycerol lipids and free fatty acids yielded decreased abundances when samples were transported at room temperature. We conclude that if samples are submitted to the same lipid extraction protocol and same transportation batch at room temperature coupled with vacuum conditions it is possible to analyze lipid extracts of bovine oocytes and still obtain informative lipid profiling results.

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Section: Discussionmentioning

confidence: 99%

Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling

de Lima,

Milazzotto,

Vireque

et al. 2023

Preprint

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show abstract

“…It is worth noting that although carnitine expression overall decreases, the age-dependent expression of butyryl carnitine and acetylcarnitine increases in oocytes [7]. Carnitine treatment contributes to the preservation of oocyte quality and the enhancement of embryo development [85][86][87][88][89]. Specifically, it decreases oocyte lipid content, boosts cellular energy supply, enhances antioxidant capacity, and actively modulates mitochondrial activity during oxidative stress [85][86][87][88][89].…”

Section: Lipid Metabolismmentioning

confidence: 99%

“…Carnitine treatment contributes to the preservation of oocyte quality and the enhancement of embryo development [85][86][87][88][89]. Specifically, it decreases oocyte lipid content, boosts cellular energy supply, enhances antioxidant capacity, and actively modulates mitochondrial activity during oxidative stress [85][86][87][88][89]. This, to some extent, prevents abnormal changes in phospholipid and sphingolipid content as oocytes develop into blastocysts [85][86][87][88][89].…”

Section: Lipid Metabolismmentioning

confidence: 99%

Ovarian aging: energy metabolism of oocytes

Bao,

Yin,

Liu

2024

J Ovarian Res

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In women who are getting older, the quantity and quality of their follicles or oocytes and decline. This is characterized by decreased ovarian reserve function (DOR), fewer remaining oocytes, and lower quality oocytes. As more women choose to delay childbirth, the decline in fertility associated with age has become a significant concern for modern women. The decline in oocyte quality is a key indicator of ovarian aging. Many studies suggest that age-related changes in oocyte energy metabolism may impact oocyte quality. Changes in oocyte energy metabolism affect adenosine 5'-triphosphate (ATP) production, but how related products and proteins influence oocyte quality remains largely unknown. This review focuses on oocyte metabolism in age-related ovarian aging and its potential impact on oocyte quality, as well as therapeutic strategies that may partially influence oocyte metabolism. This research aims to enhance our understanding of age-related changes in oocyte energy metabolism, and the identification of biomarkers and treatment methods.

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“…Maintenance of the female genotypic background is also possible to achieve with the aid of the methods applied to cryogenically preserve oocytes and embryos. The oocytes can be recovered and cryopreserved at any stage of meiotic maturation, including oocytes retrieved from primordial, preantral or antral ovarian follicles [86][87][88]. Nonetheless, cryogenic protection of oocytes is tremendously limited due to the extremely low efficiency of this biotechnique.…”

Section: The State Of the Art And A Wide Range Of Biotechnological An...mentioning

confidence: 99%

“…It is also noteworthy that, considering the vitrification-mediated cryopreservation of oocytes, the warming rate appears to play a more important role than the cooling rate. Such a finding can be explained by the fact that the viability of murine oocytes that had been subjected to vitrification under the conditions of a very slow cooling rate and high warming rate increased to a large extent, as compared to the viability of oocytes undergoing vitrification characterized by a combination of highly rapid kinetics of cooling and subsequent slow kinetics of warming [86,87,100,101]. Nevertheless, such a negative correlation has not been confirmed in the investigations aimed at the vitrification of oocytes and embryos in selected livestock species (rabbits, pigs and cattle), which exhibit a particular oversensitivity to cooling rates related to cryopreservation procedures [92,99,101,102].…”

Section: The State Of the Art And A Wide Range Of Biotechnological An...mentioning

confidence: 99%

Creating Ex Situ Protected Bioreservoirs as a Powerful Strategy for the Reproductive Biotechnology-Mediated Rescue of Threatened Polish Livestock Breeds

2023

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The current article presents the state of the art of the creation of bioreservoirs of cryopreserved somatic and stem cell lines and cryopreserved or lyophilized germplasm-based resources of selected farm animal species. It also presents the agricultural, biological and biotechnological determinants, and showcases the importance of the National Research Institute of Animal Production (NRIAP) in Poland in this process. The aforementioned bioreservoirs serve as an innovative research tool used for cryogenically or lyophilizogenically assisted and species-specific ex situ conservation. The latter ensures, in the long term, not only restitution, but also perpetuation of sustainable biodiversity that underlies genotypic and phenotypic, intra- and inter-population variability within pure-breeding herds of the national conserved livestock breeds.

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Membrane lipid changes in mouse blastocysts induced by ovarian stimulation, IVF and oocyte vitrification

Cited by 4 publications

References 58 publications

Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling

Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling

Ovarian aging: energy metabolism of oocytes

Creating Ex Situ Protected Bioreservoirs as a Powerful Strategy for the Reproductive Biotechnology-Mediated Rescue of Threatened Polish Livestock Breeds

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