Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling
Camila Bruna de Lima,
Marcella Pecora Milazzotto,
Alessandra Aparecida Vireque
et al.
Abstract:Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic development. Lipid profiling in samples of minute size, such as oocytes, is challenging but has been achieved by mass spectrometry technologies such as multiple reaction monitoring (MRM) profiling. With the goals of further simplifying sample workflow and investigating the influence of pre-analytical conditions, we have evaluated how different extra… Show more
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