Membrane Ig on MPC11 myeloma cells: correlation between the expression of membrane Ig, a receptor for Ig and the process of secretion

Corte, Giorgio; Risso, Angela; Ferrarini, Manlio; Bargellesi, A.

doi:10.1002/eji.1830060103

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…As maturation proceeds, the cells " cap " surface Jg and express first Fc and then both Fc and C3 receptors. The plasma cells, or mature secretory B cells, have inconsistent surface markers, but contain intracytoplasmic Ig (Corte et al, 1976). We and others have found identical variations in the pattern of surface Ig, Fe and C3 receptors in human material such as normal peripheral blood (Habeshaw and Young, 1975), tonsil (Siegel, Grieco and Gupta, 1974), and in lymphomas (Brouet et al, 1975;Dorfman, 1975;Jaffe et al, 1975;Stuart and Habeshaw, 1976;Habeshaw and Stuart, 1975;Moscatelli, Bricarelli and Quartino, 1976).…”

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confidence: 51%

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Habeshaw¹,

Macaulay²,

Stuart³

1977

Br J Cancer

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Summary.-The receptor patterns of cell suspensions from 29 cases of non-Hodgkin lymphoma were correlated with the histology of the nodes from which the cells were taken. Twenty-two were judged to be predominantly or largely B-cell, and because of this preponderance these were divided by a method based on the distribution of surface immunoglobulin and the expression of Fc and C3 receptors." Mature " B-cell and B-mixed tumours showing capping surface Ig with Fc and/or C3 receptors correlated well with a nodular growth pattern, and consisted of what Rappaport (1966) calls "poorly differentiated" lymphocytes equivalent to the "small cleaved " cell as defined by Lukes and Collins (1975). Ten of the 14 patients in this receptor category are alive between 12 and 30 months after diagnosis.Receptor-silent and " immature " B-cell tumours with non-capping surface Ig correlated predominantly with the Rappaport histiocytic lymphoma and Lukes and Collins' large cleaved and large non-cleaved lymphomas, though these histological categories also included a wide variety of other receptor types such as T-cell, Receptor-overlap and the single true Macrophage tumour. Five of the 11 patients with receptor-silent or immature B-cell tumours are alive between 7 and 15 months after the diagnosis.Diffuse mixed and diffuse poorly differentiated lymphocytic lymphomas in Rappaport's classification correlated poorly with receptors, mature and immature B-cell tumours being equally represented.

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supporting

confidence: 51%

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Habeshaw¹,

Macaulay²,

Stuart³

1977

Br J Cancer

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“…Mouse myeloma cell cultures have been found to be useful models for the study of immunoglobulin G, M and A synthesis, assembly, and secretion (1)(2)(3)(4). Studies of myeloma-myeloma and myeloma-B-cell hybrids and their subclones have provided considerable information about the genetics and regulation of immunoglobulin production (5)(6).…”

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confidence: 99%

Propagation and Characterization of a Rat Myeloma Cell Line Producing Immunoglobulin E in Vitro

Eberly

Gavin

1979

Experimental Biology and Medicine

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Mouse myeloma cell cultures have been found to be useful models for the study of immunoglobulin G, M and A synthesis, assembly, and secretion (1-4). Studies of myeloma-myeloma and myeloma-B-cell hybrids and their subclones have provided considerable information about the genetics and regulation of immunoglobulin production (5-6). Similar investigations concerning immunoglobulin E (IgE) have not been possible due to the unavailability of mouse IgE-myelomas and their cell lines. However, the spontaneous myeloma model in Louvain/Wsl rats, developed by H. Bazin and his colleagues (7, 8) does offer a ready source of IgE-producing myelomas. We report here on the establishment of one of these myelomas, IR 162, in cell culture, its characterization, and the development of a system for the assay of drug effects on cell growth and IgE production.Materials arid methods. Louvain/Wsl/M rats and the IR162 IgE-secreting tumor were obtained from H. Bazin, University of Louvain, Belgium. The tumor was maintained by subcutaneous passage (7), and ascites passages of the tumor were initiated by intraperitoneal injection of tumor cells. IgE secreting cell cultures were established by a procedure similar to that described by Burtonboy et al. (9). Ascites fluid was harvested aseptically; cells were sedimented by centrifugation and resuspended at a density of 3 x 105-3 x lo6 cells/ml in RPMI 1640 medium supplemented with penicillin (100 units/ml), streptomycin (0.1 mg/ml) and 20% fetal calf serum, followed by incubation at 37". Cultures were "fed" by addition of 1 or 2 ml nutrient medium every 24-48 hr depending on the number of cells and the pH of the culture fluid. Culture medium was changed at 7-to 10-day intervals by centrifuging the cells, then resuspending them in fresh medium in the original flask. Adherent cells from the ascites formed a feeder layer to which the tumor cells attached loosely. After 6 to 8 weeks the cell cultures were transferred to medium containing 10% fetal calf serum. Passages were made every 2 or 3 days by breaking up cell clumps with a pipet and diluting the cells 1 5 or 1:lO with fresh medium.Soft agar cloning of tumor cells was performed in agarose solidified RPMI 1640 medium having a final concentration of 17% fetal calf m u m , 5 x M 2-mercaptoethanol and 5 pg/ml gentamicin. 0.5% agarose was included in the base layer and 0.22% agarose in the cell layer. No cell feeder layer was required.Cells grown in vitro were washed twice in serum-free medium and injected subcutaneously or intraperitoneally into Lou/Wsl/M rats to determine whether they retained tumorigenicity. The animals were observed for a minimum of three months.

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Lymphocyte membrane immunoglobulins: similarities between human IgD and mouse IgD‐like molecules

et al. 1977

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131I‐labeled IgD‐like molecules extracted from the membrane of mouse spleen, and lymph node cells and their constitutive chains have been characterized by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis (SDS‐PAGE) followed by radioautography. Unreduced mouse IgD‐like molecules concentrated in two definite bands (IgD1 and IgD2). IgD, migrated slightly ahead of 131I‐labeled mouse membrane IgM and displayed a mobility very similar to that of 131I‐labeled IgD extracted from the membrane of human tonsil cells. IgD2 migrated faster than both IgD1 and human membrane IgD (mIgD), but slower than 14C‐labeled mouse IgG2 b used as marker. The different mobility of IgD1 and IgD2 was due to difference in size of their respective H‐chains (δ1 and δ2), as shown by SDS‐PAGE analysis of the two molecules after reduction. Mol.wt. determination of IgD1, IgD2 and of the corresponding constitutive chains are consistent with an H2L2 structure. IgD1 and IgD2 were partially replaced following prolonged dialysis by molecules with a SDS‐PAGE mobility consistent with an HL structure, IgD3. In contrast to that found for spleen and lymph node, IgD1 only was detected on the surface of bone marrow cells. Immunofluorescence experiments have shown that while the majority of mouse lymph node or spleen cells bear IgM and IgD, IgM was found to be the predominant Ig class on the surface of bone marrow cells, although a certain number of IgD‐IgM and a few IgD‐positive cells were found. Experiments where mouse lymphocytes have been treated with pronase have indicated that, in analogy with mlgD of primates, mouse IgD‐like molecules are very labile and can be removed from the cell surface by very mild proteolytic treatment.

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Membrane Ig on MPC11 myeloma cells: correlation between the expression of membrane Ig, a receptor for Ig and the process of secretion

Cited by 10 publications

References 37 publications

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Propagation and Characterization of a Rat Myeloma Cell Line Producing Immunoglobulin E in Vitro

Lymphocyte membrane immunoglobulins: similarities between human IgD and mouse IgD‐like molecules

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