Membrane fusogenic high-density lipoprotein nanoparticles

Kim, Hyungjin; Nobeyama, Tomohiro; Honda, Shinnosuke; Yasuda, Kaori; Morone, Nobuhiro; Murakami, Tatsuya

doi:10.1016/j.bbamem.2019.06.007

Cited by 7 publications

(7 citation statements)

References 49 publications

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“…In viral carriers, proteins embedded into the lipid bilayer or associated with it are directly involved in fusion induction, as well as in processes that precede or follow membrane coalescence [5,6,7,8]. Synthetic liposomes can also benefit from fusion peptides and proteins [9,10], but their presence is not mandatory for efficient membrane fusion induction. Cholesterol [11], as well as DNA in lipoplexes [12], have also been reported as membrane fusion initiators.…”

Section: Introductionmentioning

confidence: 99%

Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells

et al. 2019

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Lipid-based nanoparticles, also called vesicles or liposomes, can be used as carriers for drugs or many types of biological macromolecules, including DNA and proteins. Efficiency and speed of cargo delivery are especially high for carrier vesicles that fuse with the cellular plasma membrane. This occurs for lipid mixture containing equal amounts of the cationic lipid DOTAP and a neutral lipid with an additional few percents of an aromatic substance. The fusion ability of such particles depends on lipid composition with phosphoethanolamine (PE) lipids favoring fusion and phosphatidyl-choline (PC) lipids endocytosis. Here, we examined the effects of temperature, ionic strength, osmolality, and pH on fusion efficiency of cationic liposomes with Chinese hamster ovary (CHO) cells. The phase state of liposomes was analyzed by small angle neutron scattering (SANS). Our results showed that PC containing lipid membranes were organized in the lamellar phase. Here, fusion efficiency depended on buffer conditions and remained vanishingly small at physiological conditions. In contrast, SANS indicated the coexistence of very small (~50 nm) objects with larger, most likely lamellar structures for PE containing lipid particles. The fusion of such particles to cell membranes occurred with very high efficiency at all buffer conditions. We hypothesize that the altered phase state resulted in a highly reduced energetic barrier against fusion.

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Section: Introductionmentioning

confidence: 99%

Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells

et al. 2019

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“…Nanodisc–liposome fusion has been previously studied to gain insight into synaptic processes, to understand the fusion pore, and for its potential in drug delivery. , However, the first approach is based on utilizations of elaborate fusion machinery, and the second requires hours for significant fusion to occur. We aimed to find a simpler, rapid, and scalable method more suited for future application in in vitro artificial cell production.…”

Section: Resultsmentioning

confidence: 99%

CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs

Stępień,

Świątek,

Robles

et al. 2023

ACS Appl. Mater. Interfaces

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For the successful generative engineering of functional artificial cells, a convenient and controllable means of delivering membrane proteins into membrane lipid bilayers is necessary. Here we report a delivery system that achieves this by employing membrane protein-carrying nanodiscs and the calcium-dependent fusion of phosphatidylserine lipid membranes. We show that lipid nanodiscs can fuse a transported lipid bilayer with the lipid bilayers of small unilamellar vesicles (SUVs) or giant unilamellar vesicles (GUVs) while avoiding recipient vesicles aggregation. This is triggered by a simple, transient increase in calcium concentration, which results in efficient and rapid fusion in a one-pot reaction. Furthermore, nanodiscs can be loaded with membrane proteins that can be delivered into target SUV or GUV membranes in a detergent-independent fashion while retaining their functionality. Nanodiscs have a proven ability to carry a wide range of membrane proteins, control their oligomeric state, and are highly adaptable. Given this, our approach may be the basis for the development of useful tools that will allow bespoke delivery of membrane proteins to protocells, equipping them with the cell-like ability to exchange material across outer/subcellular membranes.

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“…Because the use of cpHDL improved the degree and rate of depolarization, improved cpHDL will certainly result in greater efficiency. Therefore, the use of recently developed cpHDL [43] that can achieve the efficient and appropriate targeted transport of CS molecules (such as cell membrane delivery efficiency) may contribute to the development of more efficient light-induced depolarization systems. For example, the absorption spectrum of TC1 solubilized with liposomes was reported to be similar to that in H 2 O containing 10% DMSO, and their extinction coefficient at the excitation wavelength was also comparable [12].…”

Section: Discussionmentioning

confidence: 99%