Membrane Fusion Through Point Defects in Bilayers

Hui, S. W.; Stewart, T.P.; Boni, Lawrence T.; Yèagle, Philip L.

doi:10.1126/science.7233185

Cited by 249 publications

(106 citation statements)

References 20 publications

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“…We refer to it as a ''modified stalk.'' A modified stalk has been pictorially proposed by others (15). We show that at an energy of about 40 kT above that of the initial stalk, the lipids of the modified stalk can reconfigure to a fusion pore-like structure.…”

mentioning

confidence: 86%

A quantitative model for membrane fusion based on low-energy intermediates

Kuzmin

Zimmerberg²,

Chizmadzhev³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

302

373

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The energetics of a fusion pathway is considered, starting from the contact site where two apposed membranes each locally protrude (as ''nipples'') toward each other. The equilibrium distance between the tips of the two nipples is determined by a balance of physical forces: repulsion caused by hydration and attraction generated by fusion proteins. The energy to create the initial stalk, caused by bending of cis monolayer leaflets, is much less when the stalk forms between nipples rather than parallel flat membranes. The stalk cannot, however, expand by bending deformations alone, because this would necessitate the creation of a hydrophobic void of prohibitively high energy. But small movements of the lipids out of the plane of their monolayers allow transformation of the stalk into a modified stalk. This intermediate, not previously considered, is a low-energy structure that can reconfigure into a fusion pore via an additional intermediate, the prepore. The lipids of this latter structure are oriented as in a fusion pore, but the bilayer is locally compressed. All membrane rearrangements occur in a discrete local region without creation of an extended hemifusion diaphragm. Importantly, all steps of the proposed pathway are energetically feasible.

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mentioning

confidence: 86%

A quantitative model for membrane fusion based on low-energy intermediates

Kuzmin

Zimmerberg²,

Chizmadzhev³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

302

373

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“…In most of these systems an isotropic 31p-NMR signal has been detected next to line shapes typical of lipids organized in the lamellar and H~I phases. Such mixtures are PE/egg PC [5], DOPE/DOPC/cholesterol [3], cardiolipin/egg PC in the presence of Ca 2+, MGlucDG/egg PC [3], total lipid extracts of E. coil [19], rod outer segments [30] and inner mitochondrial membranes [31]. Such an isotropic signal found next to bilayer and H~I phase spectra indicates isotropic motion of lipids in large structures.…”

Section: Hc H N Phase-lamellar Transition In Mixed Lipid Systemsmentioning

confidence: 99%

“…These particles have been called 'lipidic particles' since they were found in pure lipid systems, and thus must originate from a specific lipid organization. Such lipidic particles have been found in a variety of lipid mixtures and in total lipid extracts under physiological conditions in excess water [2][3][4][5][6][7][8]. A common feature of these lipid systems is that at least one of the lipids prefers to adopt the hexagonal II (Hn) phase when dispersed in pure form.…”

Section: Introductionmentioning

confidence: 99%

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Lipidic intramembranous particles

Verkleij

1984

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

411

166

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“…Siegel refined his mechanism and eliminated IMls as possible intermediates in favor of a more stable semitoroidal structure termed a stalk (Siegel, 1993c). Siegel's stalk structure is a modified version of the stalk that was originally proposed by Hui et al (Hui et al, 1982). Later Markin, Chernomordik and coworkers developed a molecular mechanism for the involvement of stalks in the Lcx/Hu transition (Markin et al, 1984;Chernomordik et al, 1985;Chernomordik et al, 1987;Leikin et al, 1987;Kozlov et al, 1989).…”

mentioning

confidence: 99%

Photoinduced Fusion of Liposomes

Bennett¹,

O’Brien²

1994

J. Am. Chem. Soc.

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Membrane Fusion Through Point Defects in Bilayers

Cited by 249 publications

References 20 publications

A quantitative model for membrane fusion based on low-energy intermediates

A quantitative model for membrane fusion based on low-energy intermediates

Lipidic intramembranous particles

Photoinduced Fusion of Liposomes

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