Membrane fouling during ultrafiltration of plasmid DNA through semipermeable membranes

Borujeni, Ehsan Espah; Zydney, Andrew L.

doi:10.1016/j.memsci.2013.08.032

Cited by 15 publications

(11 citation statements)

References 19 publications

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“…This small decline in filtrate flux (compared to the large decline in plasmid transmission) is due to fluid flow around the trapped plasmids in the partially blocked pores. This is discussed in more detail by Borujeni and Zydney [7].…”

Section: Membrane Orientationmentioning

confidence: 93%

“…Actual concentrations were determined using calibration standards included on each microplate. The quality of the plasmid stock solutions and the effectiveness of the enzymatic digestions were examined by the agarose gel electrophoresis (AGE) following the procedures described by Borujeni and Zydney [7]. AGE was also used to estimate the relative concentrations of the different isoforms in separation experiments using mixtures of the linear and supercoiled isoforms.…”

Section: Assaysmentioning

confidence: 99%

“…One of the challenges in DNA ultrafiltration is membrane fouling [7][8][9]. Borujeni and Zydney [7] showed that membrane fouling increased dramatically at high feed concentrations, with the extent of plasmid transmission decreasing rapidly during the ultrafiltration process due to the partial blockage of the membrane pores by individual plasmids that became trapped at the pore entrance.…”

Section: Introductionmentioning

confidence: 99%

“…Borujeni and Zydney [7] showed that membrane fouling increased dramatically at high feed concentrations, with the extent of plasmid transmission decreasing rapidly during the ultrafiltration process due to the partial blockage of the membrane pores by individual plasmids that became trapped at the pore entrance. The rate of fouling was greater for the larger plasmids due to the greater probability of a plasmid getting trapped at the pore entrance.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Ying

Borujeni

Zydney

2015

Journal of Membrane Science

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Section: Membrane Orientationmentioning

confidence: 93%

Section: Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Ying

Borujeni

Zydney

2015

Journal of Membrane Science

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“…Simulations show knots can jam nanopore translocation, 25 and experiments suggest jamming of knots during ultrafiltration of plasmid DNA. 47 Practically, our results could guide development of microfluidic devices that precondition molecules to unknotted states for such applications. Finally, since the model employed is parametrized to dsDNA at experimentally realizable conditions, we hope future experiments will test our results.…”

mentioning

confidence: 89%

Untying Knotted DNA with Elongational Flows

Renner

Doyle

2014

ACS Macro Lett.

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We present Brownian dynamics simulations of initially knotted double-stranded DNA molecules untying in elongational flows. We show that the motions of the knots are governed by a diffusion−convection equation by deriving scalings that collapse the simulation data. When being convected, all knots displace nonaffinely, and their rates of translation along the chain are topologically dictated. We discover that torus knots "corkscrew" when driven by flow, whereas nontorus knots do not. We show that a simple mechanism can explain a coupling between this rotation and the translation of a knot, explaining observed differences in knot translation rates. These types of knots are encountered in nanoscale manipulation of DNA, occur in biology at multiple length scales (DNA to umbilical cords), and are ubiquitous in daily life (e.g., hair). These results may have a broad impact on manipulations of such knots via flows, with applications to genomic sequencing and polymer processing.K nots are commonly encountered and manipulated in everyday experiences such as tying one's shoelaces or untangling spontaneously knotted strings. 1 Formally defined only for closed rings, the topologies of "open" knots (referred to hereafter simply as knots) are often unambiguous (e.g., shoelaces and neckties) and can be closed and algorithmically defined. 2−4 At microscopic scales, chromosomal knots are modified by topoisomerases during cell division 5 and are thought to participate in gene regulation. 6 Knots are found in proteins 7,8 and viral capsid DNA,9,10 likely with yet to be fully understood functions. It has been mathematically proven that knots become asymptotically likely as the length of a polymer increases, 11 a fact that explains their ubiquity.Due to the emerging significance of knotted polymers, a growing body of simulation literature is devoted to their study. 12,13 For instance, while the topology of a ring is fixed, an open polymer can spontaneously form and untie knots. 14 The probability of forming such knots can be nonmonotonic when the polymer is confined in slits 15,16 or tubes, 17 and increasing the stiffness of a polymer can similarly influence the knotting probability in unintuitive ways. 18,19 Such knots can substantially affect the mechanical properties 20,21 and rheological behavior 22 of a polymer, and the probability of forming knots has been used to infer the effective diameter of DNA molecules. 23 Recently, simulations have shown dramatic slowing of processes wherein a knot is driven along a chain such as entropic ejection of DNA from a viral capsid 24 and the translocation of single-stranded DNA (ssDNA) 25 and polypeptides 26 through pores.Common nanofluidic experiments have led to the spontaneous formation of knots in DNA by collision with channel defects 27 or the application of moderate electric fields 28,29 during electrophoresis. More broadly, the growing library of methods to manipulate DNA molecules in nanofluidic devices has enabled fundamental research about single polymer molecules. 30,31 Th...

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Nanoporous membranes generated from self‐assembled block polymer precursors: Quo Vadis?

Zhang

Sargent

Boudouris

et al. 2014

J of Applied Polymer Sci

103

135

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Nanoporous membranes based on self‐assembled block polymer precursors are an emerging class of promising separation, purification, and sensing devices due to the ability of researchers to control the nanostructure and chemistry of these multifunctional materials and devices. In fact, modern polymer chemistry provides techniques for the facile, controlled synthesis of the block polymers that constitute these devices. These designer macromolecules, in turn, can then self‐assemble into functional nanostructures depending upon the chemical identity of the synthesized block polymers and the thin film fabrication methods employed. After fabrication, these nanoporous membranes offer a highly tunable platform for applications that require high throughput, high surface area, homogeneous pore size, and varying material properties. And, with these readily tunable chemical and structural properties, block polymer membranes will allow for significant improvements in myriad applications. In this Review, we summarize the key advances, with a specific emphasis on the previous 5 years of work, that have allowed block polymer‐based membranes to reach their current level of technology. Furthermore, we project how these state‐of‐art, self‐assembled block polymer membrane technologies can be utilized in present‐day and future application arenas. In this way, we aim to demonstrate that the rigorous work performed on block polymer‐based membranes has laid a strong foundation that will allow these macromolecular systems to: (1) be major avenues of fundamental scientific research and (2) be parlayed into transferable technologies for the betterment of society in the imminent future. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41683.

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Membrane fouling during ultrafiltration of plasmid DNA through semipermeable membranes

Cited by 15 publications

References 19 publications

Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Untying Knotted DNA with Elongational Flows

Nanoporous membranes generated from self‐assembled block polymer precursors: Quo Vadis?

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