Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Ying, Lei; Borujeni, Ehsan Espah; Zydney, Andrew L.

doi:10.1016/j.memsci.2015.01.029

Cited by 10 publications

(12 citation statements)

References 16 publications

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“…The filtrate flux remained stable at each applied pressure and the hydraulic permeability of the membrane after the ultrafiltration experiment (evaluated from data for the filtrate flux as a function of the transmembrane pressure) was within 10% of that for the clean membrane. The absence of any fouling is consistent with previous results obtained with similarly dilute plasmid solutions (Li et al, ).…”

Section: Resultssupporting

confidence: 92%

Enhanced purification of plasmid DNA isoforms by exploiting ionic strength effects during ultrafiltration

Currie

Zydney

2015

Biotech & Bioengineering

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The solution structure of plasmid DNA is known to be a strong function of solution conditions due to intramolecular electrostatic interactions between the charged phosphate groups along the DNA backbone. The objective of this work was to determine whether it was possible to enhance the use of ultrafiltration for separation of different plasmid isoforms by proper selection of the solution ionic strength and ion type. Experiments were performed with a 3.0 kbp plasmid using composite regenerated cellulose ultrafiltration membranes. The transmission of the linear isoform was nearly independent of solution ionic strength, but increased significantly with increasing filtrate flux due to the elongation of the highly flexible plasmid in the converging flow field into the membrane pores. In contrast, the transmission of the open-circular and supercoiled plasmids both increased with increasing NaCl or MgCl2 concentration due to the change in plasmid size and conformational flexibility. The effect of ionic strength was greatest for the supercoiled plasmid, providing opportunities for enhanced purification of this therapeutically active isoform. This behavior was confirmed using experiments performed with binary mixtures of the different isoforms. These results clearly demonstrate the potential for enhancing the performance of membrane systems for plasmid DNA separations by proper selection of the ionic conditions.

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Section: Resultssupporting

confidence: 92%

Enhanced purification of plasmid DNA isoforms by exploiting ionic strength effects during ultrafiltration

Currie

Zydney

2015

Biotech & Bioengineering

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“…However, this energy can also reflect the potential energy that an object must acquire in order to be transported in the map. For example, this energy can represent the stretching energy that is required to deform a plasmid DNA and, then, to enable its penetration inside a channel (Li et al, 2015). This energy can also represent the energy needed for a molecule to be solubilized in a material (as in the diffusion/solubilization model in reverse osmosis).…”

Section: The Energy Map: An Ingredient To Understand Complex Mechanismsmentioning

confidence: 99%

“…The small discrepancies could result from the way the partition is accounted for: with a ramp of potential in the simulation and with a discontinuity in the concentration profile in the analytical expression. The effect of the slope of the ramp, that could explain the facilitated transfer observed when working with pores that exhibit a conic shape of an hour glass shape (Gravelle et al, 2013;Li et al, 2015), will be further investigated.…”

Section: Case Study: Transient Transfer Of a Colloid Through A Membranementioning

confidence: 99%

An energy map model for colloid transport

Bacchin

2017

Chemical Engineering Science

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When dispersed colloids are flowing, they experience interactions with the fluid (friction) and with other colloids (surface interactions). These phenomena are usually taken into account through a Suspension Balance Model (SBM) that couples mass and momentum balances. However, in many applications, the dispersed particles flow close to an interface or inside a porous media. The flow in such a confined environment leads to significant particle-wall interactions. This paper puts forward an energy map model that accounts for these particle-wall interactions. A way to implement the energy map in the SBM is to introduce an interfacial pressure concept. The new possibilities opened up by the energy map that account for interfacial interaction in the SBM are analysed. A transient 1D case study for the transfer of colloids through a pore illustrates the potentialities of the Suspension Balance Model integrating an Energy Map (SBM-EM). The model enables the description of the transmission of the colloids through the energy map representing the membrane (mass balance) and the consequences in terms of an out-of-equilibrium counter pressure (momentum balance). The counter osmotic pressure is then explained by the interfacial interaction between the colloids and the interface; these interfacial interactions that prevents the colloids from leaving the bulk volume generate forces that are transmitted to the fluid (via the drag force), thus inducing osmosis. The energy map model can enable the incorporation of the physical and chemical heterogeneities of the interacting surfaces. It might be of interest to explore the transfer of colloids along or inside real surfaces (being a mosaic of nano-or micro-scale domaines with specific interactions).

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“…The shift in transmission described by the model in Figure 6 has been observed in filtration experiments carried out in two different directions [ 48 ]: with the flow directed from the bulk to the skin layer of the membrane (the interaction occurs abruptly at the pore entrance)—referred to as forward filtration and corresponding to Figure 5 c. with the flow directed through the open pores in the substructure before the skin (the interaction with the pore wall take place progressively)—referred to as reverse filtration and corresponding to Figure 5 d. …”

Section: Impact Of Energy Landscape Stiffnessmentioning

confidence: 99%

“…The experimental transmission of plasmid DNA obtained by Li et al [ 48 ] in forward and reverse filtration are represented by symbols in Figure 7 . The results demonstrate that the transmission of the protein is facilitated when the filtration is performed in the reverse direction ( Figure 5 d).…”

Section: Impact Of Energy Landscape Stiffnessmentioning

confidence: 99%

Membranes: A Variety of Energy Landscapes for Many Transfer Opportunities

Bacchin

2018

Membranes

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A membrane can be represented by an energy landscape that solutes or colloids must cross. A model accounting for the momentum and the mass balances in the membrane energy landscape establishes a new way of writing for the Darcy law. The counter-pressure in the Darcy law is no longer written as the result of an osmotic pressure difference but rather as a function of colloid-membrane interactions. The ability of the model to describe the physics of the filtration is discussed in detail. This model is solved in a simplified energy landscape to derive analytical relationships that describe the selectivity and the counter-pressure from ab initio operating conditions. The model shows that the stiffness of the energy landscape has an impact on the process efficiency: a gradual increase in interactions (such as with hourglass pore shape) can reduce the separation energetic cost. It allows the introduction of a new paradigm to increase membrane efficiency: the accumulation that is inherent to the separation must be distributed across the membrane. Asymmetric interactions thus lead to direction-dependent transfer properties and the membrane exhibits diode behavior. These new transfer opportunities are discussed.

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Use of preconditioning to control membrane fouling and enhance performance during ultrafiltration of plasmid DNA

Cited by 10 publications

References 16 publications

Enhanced purification of plasmid DNA isoforms by exploiting ionic strength effects during ultrafiltration

Enhanced purification of plasmid DNA isoforms by exploiting ionic strength effects during ultrafiltration

An energy map model for colloid transport

Membranes: A Variety of Energy Landscapes for Many Transfer Opportunities

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