Membrane fluorescent probes for the demonstration of lymphocyte population heterogeneity. I. T and B lymphocytes of mice and rats

Korkina, Liudmila; Dobretsov, G. E.; Walzel, Gabriel; Kogan, É. M.; Zimin, Yu. I.; Vladimirov, Yu. A.

doi:10.1016/0022-1759(81)90301-x

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…There is evidence, for example, for differences between various lymph nodes, including factors such as the ratio of T:B cells (Korkina et al, 1981), local cytokine environment (Daynes et al, 1990;Taguchi et al, 1990), and neurotransmitter-specific innervation (Bellinger et al, 2001). All of these factors could influence the development of antigen specific antibodies and how stress may affect this response.…”

Section: Discussionmentioning

confidence: 98%

Stressor Exposure Produces Long-term Reductions in Antigen-specific T and B Cell Responses

Gazda

Smith

Watkins

et al. 2003

Stress

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Exposure to an acute laboratory stressor at the time of keyhole limpet hemocyanin (KLH) immunization results in a long-term suppression in circulating anti-KLH antibody. The mechanism for the stress-induced reduction in anti-KLH immunoglobulin (Ig) remains unknown. Given that the generation of anti-KLH antibody requires T cell help, we hypothesize that stress reduces the proliferation of anti-KLH T cells, thus leading to a reduction in anti-KLH antibody. The present studies examined the effect of tail shock stress (100, 1.6 mA, 5-s, 60 s ITI) on the KLH specific T cell response. Fischer F344 rats were immunized either intraperitoneally (i.p.) or subcutaneously (s.c.) at the base of the tail with 200 microg KLH, and exposed to inescapable tail shock (IS) or remained in their home cages (HCC). T cell proliferation after KLH restimulation, but not ConA, was markedly suppressed in IS animals in both the spleen after i.p. immunization and the draining lymph nodes after s.c. immunization. Other secondary lymphoid cells did not differ in their proliferative capacity. Anti-KLH IgG, IgG1 and IgG2a, but not anti-KLH IgM serum levels were significantly suppressed. These data support the conclusion that stress suppresses the generation of antigen specific T cells. In addition, the methods employed in the current study allow the isolation of the site of the acquired T cell immune response, making it possible to further elucidate the cellular mechanisms that contribute to stress-induced modulation of the antigen-specific acquired immune response.

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Section: Discussionmentioning

confidence: 98%

Stressor Exposure Produces Long-term Reductions in Antigen-specific T and B Cell Responses

Gazda

Smith

Watkins

et al. 2003

Stress

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“…There are now dyes available probing the functional state of membranes (12) or of mitochondria (6,19); these dyes could also be of significant value for screening. …”

Section: Discussionmentioning

confidence: 99%

Use of flow cytometry in industrial microbiology for strain improvement programs

1984

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A flow cytometry (FCM) system was chosen to analyze and sort microbiological samples, e.g., bacteria, bacterial spores, yeasts, and fungal spores, without major changes in the commercially available state. The system was further improved by addition of a stepping motor-driven scanning table that accepts standard petri dishes or microtiter plates. The electronics of the sorting system were changed to enable the sorter to deliver only one particle at a time, working in a "handshake" mode with the scanning table. Appropriate parameters, depending on the biological material and including all fluorescent stains that do not impair growth and productivity of cells were chosen to sort distinct bioparticles under aseptic conditions and to clone colonies or cultures out of them. A mutagenized sample of spores entering the germination cycle can be followed and thus provide a means to pick only viable growing cells despite the killing effect of the mutagen. One example of a typical strain improvement is illustrated. From a spore suspension of Rhizopus arrhizus, a subpopulation of morphologically different spores comprising about 5-10% of the whole population was cloned. From approximately 8,000 clones, 10 were isolated that produced approximately five-to six-fold the amount of fungal lipase activity, compared to the original strain or to reisolated clones from the mean population of clones.

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Ryodipine ? A new fluorescent probe for detecting differences between lymphocytes

Belevich¹,

Kosnikov²,

Dubur³

et al. 1989

Bull Exp Biol Med

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Membrane fluorescent probes for the demonstration of lymphocyte population heterogeneity. I. T and B lymphocytes of mice and rats

Cited by 9 publications

References 17 publications

Stressor Exposure Produces Long-term Reductions in Antigen-specific T and B Cell Responses

Stressor Exposure Produces Long-term Reductions in Antigen-specific T and B Cell Responses

Use of flow cytometry in industrial microbiology for strain improvement programs

Ryodipine ? A new fluorescent probe for detecting differences between lymphocytes

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