Membrane-enclosed electroenzymatic catalysis with a low molecular weight electron-transfer mediator

Grimes, Michael T.; Drueckhammer, Dale G.

doi:10.1021/jo00074a056

Cited by 24 publications

(11 citation statements)

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“…Currently, the most common clinical method for measuring blood lactic acid involves collecting a blood sample and analyzing the sample by an enzyme-mediated electrochemical method. [8] Blood samples need to be preserved on ice and centrifuged immediately to avoid lactic acid reduction by red blood cells. It is inconvenient to monitor lactic acid continuously using traditional methods.…”

Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

Hsu

Chiang²

2010

J Raman Spectroscopy

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Lactic acid is a simple and effective indicator for estimating physiological function. Rapid and sensitive detection of lactic acid is very useful in clinical diagnosis. However, the concentration of lactic acid in the physiological state is too low to be detected using traditional Raman spectroscopy. We applied silver colloidal nanoparticles-mediated surface-enhanced Raman spectroscopy (SERS) for rapid identification and quantification of lactic acid. The standard SERS spectra of lactic acid were defined and the 1395 cm −1 band intensity was used for quantification from 0.3 to 2 mM (R 2 = 0.99). In clinical blood sample measurement, the ultrafiltration (cutoff value 5 kDa) can efficiently reduce background fluorescence to improve SERS performance. We established identical and optimal procedure by adjusting reaction time and volume ratio of serum and nanoparticles to obtain high SERS reproducibility. Finally, we showed that silver colloidal nanoparticles-mediated SERS technique was successfully applied to detect lactic acid at physiological concentrations in the blood.

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Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

Hsu

Chiang²

2010

J Raman Spectroscopy

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“…In a compact reactor, nitrogen bubbling is no longer a problem, because it can be performed in the storage tank where no enzyme is present; the enzyme, confined near the electrode surface, is not in contact with the dispersed gas phase. To our knowledge, the first reactor of this type was proposed by Grimes and Drueckhammer [39]. The working electrode was simply inserted in a dialysis tube and the results were promising.…”

Section: Membrane Electrochemical Reactorsmentioning

confidence: 99%

Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness

Délécouls-Servat

Bergel

Basseguy

2004

Bioprocess Biosyst Eng

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Two membrane electrochemical reactors (MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as electrochemical mediator. A semipermeable membrane (dialysis or ultra-filtration) was integrated in the filter-press electrochemical reactor to confine the enzyme(s) as close as possible to the electrode surface. When methyl viologen was used, the transformation ratio of cyclohexanone varied from 0 to 65% depending on the internal arrangement of the reactor. Matching the reactor configuration to the reaction system was essential in this case. With the rhodium complex, the ultra-filtration MER was tested in continuous and recycling configurations. The best conditions led to 100% transformation of 0.1 L volume of 0.1 M cyclohexanone after 70 h with the recycling mode. Finally, the performances of the reactors are discussed with respect to different evaluations of the production yields.

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“…Membrane-enclosed electroenzymatic synthesis using L-LDH (entrapped together with LiDH in a dialysis membrane) in order to prepare L-lactate and α-hydroxy butyrate was reported too [149].…”

Section: Syntheses Of α-Hydroxy Acidsmentioning

confidence: 99%

“…Membranetype immobilized enzyme reactors (with glucose oxidase, glutaminase, and laccase, respectively) were used in flowinjection analysis for the monitoring of glucose (during continuous cultivation of yeast), for ammonia and glutamate (during mammalian cell culture), and for studying the properties of immobilized laccase [283]. More recently a membrane-enclosed procedure was used in the conversion of pyruvate to L-lactate, catalyzed by L-LDH, and coupled to NADH regeneration using LiDH and and a low molecular weight mediator [149]. PEG-NAD + was immobilized in a membrane reactor together with a crude preparation of two diketocamphane monooxygenases from Pseudomonas putida.…”

Section: Reactors For Redox Reactions Coupled To Coenzyme Regenerationmentioning

confidence: 99%

Redox Enzymes Used in Chiral Syntheses Coupled to Coenzyme Regeneration

Leonida¹

2001

CMC

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Due to their ability to perform reactions in a stereo- and regiospecific manner, while functioning under mild conditions of temperature and pH, enzymes are increasingly used for chiral synthesis in the pharmaceutical industry, in medicinal chemistry, agriculture, cosmetic and food industry. The oxidoreductases as catalysts require a coenzyme (cofactor) which functions as an electron carrier. If used in stoichiometric amounts the cost of coenzymes makes synthetic applications of redox enzymes prohibitively expensive for industrial applications. The present review updates previous discussions about the objectives of cofactor regeneration as a requirement for the applicability of enzymatic redox reactions, and about the strategies customarily used to this end. Different types of chiral syntheses coupled successfully to coenzyme regeneration (amino acid synthesis, lactone synthesis, synthetic reactions involving alcohols, hydroxy acid and steroid synthesis, deracemization) are then discussed. New catalysts, cross-linked enzyme crystals, prepared from redox enzyme are presented in a small paragraph together with some of their applications. Special attention is given to whole cells used in this type of synthesis and their advantages and disadvantages are presented in one paragraph. Finally, different reactors, within which were conducted chiral syntheses catalyzed by oxireductases and coupled to cofactor regeneration, are briefly discussed.

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Membrane-enclosed electroenzymatic catalysis with a low molecular weight electron-transfer mediator

Cited by 24 publications

References 0 publications

Surface‐enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

Surface‐enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness

Redox Enzymes Used in Chiral Syntheses Coupled to Coenzyme Regeneration

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