Membrane cell disruption of Candida albicans by Masoyi bark essential oil

Abstract

Essential oils distilled from the bark of Massoia aromatica Becc. (Masoyi) has been known from our previous research as a potential towards both and states. This study aims to determine essential oil’s mechanism of action towards Candida ATCC 10231. Observation of the mechanism towards C. was performed by Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) following the essential oil application in sub-minimum inhibition concentration, in comparison to untreated cells as the control. Considering that both and of C. were influenced, the effect on production was also observed using thin layer chromatography of the microbial . Following SEM and TEM results, the effect on membrane permeability seems to play a role in the activity. To support the result, an experiment using influx Propidium Iodide Test was performed. TLC and GC-MS analysis showed that C-10 is a major constituent of oil. Observation of C. cells by using SEM showed cell shrinkage, which may be due to disruption in membrane cell permeability. TEM confirmed the membrane cell disruption, which causes cytoplasmic leakage. The result of the iodide influx test confirmed the damage to membrane permeability. The decrease in production was observed, which may play a role in the activity of the essential oil.

Essential Oils against Candida auris—A Promising Approach for Antifungal Activity

“…The position of the-OH group within the molecule is also critical, as the phenolic-OH group attached directly to the benzene ring (forming phenol) is more effective in damaging membranes than an -OH group attached to the benzene ring via a methylene bridge [ 20 ]. Electron microscopy and transmission electron microscopy analyses were used to demonstrate that Massoia aromatica EO caused cell shrinkage, cytoplasmic leakage, and changes in cell shape of C. albicans , also increasing membrane permeability and leading to the leakage of cytosol and eventual rupture of the cell membrane [ 21 ].…”

“…The EO from Massoia aromatic was found to inhibit farnesol, a quorum-sensing molecule that is necessary for biofilm formation in C. albicans . This inhibition may play a role in the anti-biofilm activity of EOs [ 21 ]. A summary of the mechanisms of antifungal actions of EOs and their chemical constituents is provided in Figure 2 .…”