Membrane bending elasticity and its role for shape fluctuations and shape transformations of cells and vesicles

Sackmann, E.; Duwe, H. P.; Engelhardt, H.

doi:10.1039/dc9868100281

Cited by 130 publications

(63 citation statements)

References 3 publications

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“…Such fluid-like properties would allow large membrane cirvature without abnormal increases in permeability. Presumably, this desirable property would be facilitated by rapid redistribution of molecular species in response to local stresses as discussed recently by Sackmann et al (48).…”

Section: Plasma Membranes In Eucaryotic Cells -The Second Stage Of Mementioning

confidence: 99%

The evolution of membranes

Bloom¹,

Mouritsen²

1988

Can. J. Chem.

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. J. Chem. 66, 706 (1988).Consideration of the influence of cholesterol on the physical properties of biological membranes leads to the conclusion that cholesterol increases the thickness of fluid membrane bilayers without appreciably increasing the microviscosity component of membrane fluidity. At sufficiently high cholesterol concentrations, the gel-liquid crystalline phase transition is completely eliminated in phospholipid-cholesterol mixtures and the system has the properties of a two-dimensional liquid over a wide range of temperatures. It is proposed that with the evolution of cholesterol and related sterols in an oxygen-rich atmosphere, the resulting modification of physical constraints on membrane properties made it possible for new evolutionary driving forces to manifest themselves leading to the peculiar properties of plasma membranes of eucaryotic cells.MYER BLOOM et OLE G. MOURITSEN. Can. J. Chem. 66, 706 (1988).Si on examine l'influence du cholestCro1 sur les propriCtCs physiques des membranes biologiques, on est amen6 B conclure que le cholesterol augmente 1'Cpaisseur des doubles couches des membranes fluides sans alfgmenter d'une facon appriciable la composante de la microviscosit6 de la fluidit6 de la membrane. A des concentrations de cholestCro1 suffisamment ClevCes, la transition de la phase cristalline gel-liquide est complktement CliminCe des mClanges phospholipide-cholestCro1 et, pour un grand intervalle de tempiratures, le systkme posskde les propriCtCs d'une liquide bi-dimensionnel. On est amen6 B proposer que, avec 1'Cvolution du cholestCro1 et des stCrols apparentCs dans un atmosphkre riche en oxygkne, la modification qui rCsulte de contraintes physiques sur les propriCtCs de la membrane fait qu'il est possible pour de nouvelles forces Cvolutionnaires de se manifester; ceci provoque l'apparition de propriitis particulikres pour les membranes plasmatiques des cellules eucaryotices.[Traduit par la revue]

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Section: Plasma Membranes In Eucaryotic Cells -The Second Stage Of Mementioning

confidence: 99%

The evolution of membranes

Bloom¹,

Mouritsen²

1988

Can. J. Chem.

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“…Here, k is the bending rigidity, also called bending elastic modulus. For phosphatidylcholine bilayers in the liquid phase k is typically 10 À19 J [782][783][784][785][786]. For the spontaneous adsorption of a bilayer, the adsorption energy must be higher than the bending energy.…”

Section: Force Curves On Lipid Bilayersmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,198

2,949

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“…Micron-scale fluid-phase lipid-bilayer vesicles have been observed in recent years under controlled laboratory conditions [1][2][3][4][5][6][7][8][9][10][11] to exhibit many amusing and diverse shapes. At the same time, there is now a one-parameter theory of vesicle shapes, the so-called areadifference-elasticity (ADE) model [12][13][14][15], which appears to be qualitatively consistent with available experimental observations.…”

Section: Introductionmentioning

confidence: 99%

Mapping vesicle shapes into the phase diagram: A comparison of experiment and theory

et al. 1997

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Phase-contrast microscopy is used to monitor the shapes of micron-scale fluid-phase phospholipid-bilayer vesicles in aqueous solution. At fixed temperature, each vesicle undergoes thermal shape fluctuations. We are able experimentally to characterize the thermal shape ensemble by digitizing the vesicle outline in real time and storing the time-sequence of images. Analysis of this ensemble using the area-difference-elasticity (ADE) model of vesicle shapes allows us to associate (map) each time-sequence to a point in the zerotemperature (shape) phase diagram. Changing the laboratory temperature modifies the control parameters (area, volume, etc.) of each vesicle, so it sweeps out a trajectory across the theoretical phase diagram. It is a nontrivial test of the ADE model to check that these trajectories remain confined to regions of the phase diagram where the corresponding shapes are locally stable. In particular, we study the thermal trajectories of three prolate vesicles which, upon heating, experienced a mechanical instability leading to budding. We verify that the position of the observed instability and the geometry of the budded shape are in reasonable accord with the theoretical predictions. The inability of previous experiments to detect the "hidden" control parameters (relaxed area difference and spontaneous curvature) make this the first direct quantitative confrontation between vesicle-shape theory and experiment. submitted to PRE Typeset using REVT E X 1

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Membrane bending elasticity and its role for shape fluctuations and shape transformations of cells and vesicles

Cited by 130 publications

References 3 publications

The evolution of membranes

The evolution of membranes

Force measurements with the atomic force microscope: Technique, interpretation and applications

Mapping vesicle shapes into the phase diagram: A comparison of experiment and theory

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