Membranas biológicas homólogas preservadas em solução alcalina seguida de liofilização, glicerina a 98% e por liofilização para implantação em eqüinos

Vulcani, Valcinir Aloisio Scolla; Macoris, Delphim da Graça; Plepis, Ana Maria de Guzzi

doi:10.1590/s0103-84782008000500020

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…The amount of denaturation temperature (Td) was obtained by the curve inflexion. 29 Evaluation of the morphology. Scanning electron microscopy (SEM) was used to analyze the collagen sponge morphology (surface and cross-section).…”

Section: Preparation and Characterization Of The Pmcsmentioning

confidence: 99%

Red light accelerates the formation of a human dermal equivalent

et al. 2018

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Development of biomaterials' substitutes and/or equivalents to mimic normal tissue is a current challenge in tissue engineering. Thus, three-dimensional cell culture using type I collagen as a polymeric matrix cell support designed to promote cell proliferation and differentiation was employed to create a dermal equivalent in vitro, as well to evaluate the photobiomodulation using red light. Polymeric matrix cell support was prepared from porcine serous collagen (1.1%) hydrolyzed for 96 h. The biomaterial exhibited porosity of 95%, a median pore of 44 µm and channels with an average distance between the walls of 78 ± 14 µm. The absorption of culture medium was 95%, and the sponge showed no cytotoxicity to Vero cells, a non-tumor cell line. Additionally, it was observed that irradiation with light at 630 nm (fluency 30 J cm) leads to the cellular photobiomodulation in both monolayer and human dermal equivalent (three-dimensional cell culture system). It was also verified that the cells cultured in the presence of the polymeric matrix cell support, allows differentiation and extracellular matrix secretion. Therefore, the results showed that the collagen sponge used as polymeric matrix cell support and the photobiomodulation at 630 nm are efficient for the production of a reconstructed human dermal equivalent in vitro.

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Section: Preparation and Characterization Of The Pmcsmentioning

confidence: 99%

Red light accelerates the formation of a human dermal equivalent

et al. 2018

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“…Estos tejidos son conocidos como membranas biológicas y son obtenidos de animales donantes, la mayor parte de ellos sin vida (11,12). Las membranas biológicas han sido frecuentemente utilizadas en medicina humana; en medicina veterinaria, su uso se conoce desde 1967, cuando Pigossi (13) empleó duramadre homóloga, conservada en glicerina en perros.…”

Section: Introductionunclassified

Implantes de membranas biológicas en cirugía reconstructiva veterinaria: aspectos básicos y métodos de conservación

Piso¹,

Restán²,

Padilla³

2015

Rev. Med. Vet.

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ResumenLas membranas biológicas se emplean como implantes en cirugía veterinaria reconstructiva con el objetivo de restablecer la función y la estructura de tejidos dañados. Diversos tejidos obtenidos de animales, conservados por diferentes técnicas e implantados en receptores de la misma o de diferente especie, permiten reparar heridas en las que es evidente la extensa pérdida tisular o la imposibilidad de inducir cicatrización por primera intención. Aunque las bondades de las membranas biológicas son mayores que sus desventajas, su uso en la rutina clínica y quirúrgica no es frecuente, en gran parte por el desconocimiento de sus características, manipulación e implantación. La presente revisión pretende recopilar los aspectos generales que envuelven las membranas biológicas, desde su obtención hasta las posibles complicaciones de su uso, a través de estudios experimentales y reportes de caso relatados con anterioridad, e incentivar su uso como biomaterial de implante en defectos anatómicos, heridas traumáticas, postoperatorios oncológicos y traumas en general.Palabras clave: animales, cirugía, membranas biológicas, reconstrucción, reparación. Biological membrane implants in veterinary reconstructive surgery: Basic aspects and conservation methods AbstractBiological membranes are used as implants in veterinary reconstructive surgery in order to restore the function and structure of damaged tissues. Various tissues from animals, kept by different techniques and implanted in recipients of the same or different species, help repair wounds where extensive tissue loss or the inability to scarring is evidenced. Although the benefits of biological membranes outweigh their disadvantages, their use in clinical and surgical routines is rare, largely due to ignorance of their characteristics, handling and implantation. The present article aims to review general aspects on biological membranes, from procurement to possible complications of their use, through experimental studies and previously reported cases, and to encourage their use as a biomaterial implant in case of anatomical defects, traumatic wounds, oncological postoperative care, and traumas in general.

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“…A razão de aquecimento foi de 10 °C min -1 , na faixa de 5 a 120 °C, em atmosfera de N 2 . Foi utilizada uma massa com cerca de 10 mg, em suporte de alumínio hermético e o valor da temperatura de desnaturação (T d ) foi obtido pela inflexão da curva(VULCANI;MACORIS;PLEPIS, 2008). O experimento 12.…”

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Derme reconstituída (equivalente) <i>in vitro</i>

Oliveira¹

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The development of biomaterials substitutes and/or equivalents to mimic normal tissue is a currently challenge in tissue engineering. Studies using cell monolayer culture presents limitations with respect to two-dimensional interactions between the cells, and experiments using animals cannot predict results in humans, due to the high viability, thus compromising their clinical relevance. In consequence, threedimensional cell culture (3D) using a biomaterial designed to promote cell proliferation and differentiation has been used to recreate the complexity of a normal tissue, allowing a larger and complex cellular interaction. Aiming to mimic the in vivo environment, the present work refers to create a reconstituted dermis (dermal equivalent) in vitro using collagen, the most abundant component of the dermis, as biological matrix, as support for human fibroblasts, as well evaluate the photobiomodulation with light at 630 nm. First, a sponge was prepared from serous 1.1% porcine collagen hydrolyzed for 96 h. The biomaterial was characterized by determination of its porosity, pore diameter, the fluid absorption and the biocompatibility assays, since these parameters are important to the cell proliferation and differentiation resulting in the in vitro tissue formation. The biomaterial showed porosity of 95.2%, with a median pore of 44 µM estimated by mercury porosimetry injection, and channels with an average distance between the walls of 7814 µM estimated by SEM. These values are considered as ideal for a biosupport fibroblast growth. The absorption of water and growth medium was 95%, and the sponge showed no cytotoxicity for the Vero cell line. Additionally, it was investigated the effect of irradiation in 3D culture with red light (dose 30 J cm-2), that showed photobiomodulation on the dose 30 J cm-2 , for culturing cells in monolayer and in the early-stage of the cell growth in three-dimensional culture. By confocal microscopy, it was verified that the cells cultured in the presence of the sponge (3D culture), allows differentiation and extracellular matrix secretion. Therefore, the results showed that the collagen sponge used as a biomaterial for cell support and the photobiomodulation at 630 nm and dose of 30 J cm-2 are efficient for the production of a reconstructed dermis (equivalent) in vitro.

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Membranas biológicas homólogas preservadas em solução alcalina seguida de liofilização, glicerina a 98% e por liofilização para implantação em eqüinos

Cited by 4 publications

References 8 publications

Red light accelerates the formation of a human dermal equivalent

Red light accelerates the formation of a human dermal equivalent

Implantes de membranas biológicas en cirugía reconstructiva veterinaria: aspectos básicos y métodos de conservación

Derme reconstituída (equivalente) <i>in vitro</i>

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