Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland

Korzhenkov, Aleksei A.; Teplyuk, A. V.; Lebedinsky, Alexander V.; Khvashchevskaya, A. A.; Kopylova, Yu. G.; Аракчаа, К. Д.; Golyshin, Peter N.; Lunev, Evgenii A.; Golyshina, Olga V.; Kublanov, Ilya V.; Toshchakov, Stepan V.; Гаврилов, С. Н.

doi:10.1134/s0026261718060115

Cited by 8 publications

(4 citation statements)

References 29 publications

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“…For example, sequences assigned to the class Acetothermiia closely match with DNA sequences previously found in hydrothermal sediments (GenBank: FM868292). Members of this class were previously detected in anaerobic digesters, hot springs, and other deep biosphere studies ( Takami et al, 2012 ; Jungbluth et al, 2017 ; Zaitseva et al, 2017 ; Hao et al, 2018 ; Korzhenkov et al, 2018 ). Sequences assigned to the genus BRH-c8a from the family Desulfallas-Sporotomaculum match closely with sequences retrieved from deep groundwater (LC179584) and group with the genus Desulfotomaculum that can be found in deep subsurface environments ( Sousa et al, 2018 ; Watanabe et al, 2018 ).…”

Section: Discussionmentioning

confidence: 81%

Basalt-Hosted Microbial Communities in the Subsurface of the Young Volcanic Island of Surtsey, Iceland

et al. 2021

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The island of Surtsey was formed in 1963–1967 on the offshore Icelandic volcanic rift zone. It offers a unique opportunity to study the subsurface biosphere in newly formed oceanic crust and an associated hydrothermal-seawater system, whose maximum temperature is currently above 120°C at about 100m below surface. Here, we present new insights into the diversity, distribution, and abundance of microorganisms in the subsurface of the island, 50years after its creation. Samples, including basaltic tuff drill cores and associated fluids acquired at successive depths as well as surface fumes from fumaroles, were collected during expedition 5059 of the International Continental Scientific Drilling Program specifically designed to collect microbiological samples. Results of this microbial survey are investigated with 16S rRNA gene amplicon sequencing and scanning electron microscopy. To distinguish endemic microbial taxa of subsurface rocks from potential contaminants present in the drilling fluid, we use both methodological and computational strategies. Our 16S rRNA gene analysis results expose diverse and distinct microbial communities in the drill cores and the borehole fluid samples, which harbor thermophiles in high abundance. Whereas some taxonomic lineages detected across these habitats remain uncharacterized (e.g., Acetothermiia, Ammonifexales), our results highlight potential residents of the subsurface that could be identified at lower taxonomic rank such as Thermaerobacter, BRH-c8a (Desulfallas-Sporotomaculum), Thioalkalimicrobium, and Sulfurospirillum. Microscopy images reveal possible biotic structures attached to the basaltic substrate. Finally, microbial colonization of the newly formed basaltic crust and the metabolic potential are discussed on the basis of the data.

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Section: Discussionmentioning

confidence: 81%

Basalt-Hosted Microbial Communities in the Subsurface of the Young Volcanic Island of Surtsey, Iceland

et al. 2021

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“…Metagenomic bins with completeness more than 50% are shown in table 1. YAMB binnnig results are comparable to the results of binning using CONCOCT on the same data in terms of completeness and contamination of metagenomics bins (from 77.21 to 98.78% and from 0.92 to 3.39% respectively for five most complete bins) (Korzhenkov A. et al, 2018). Provisional taxonomical attribution was performed using NCBI BLAST (Johnson, M. et al, 2008) in blastx mode.…”

Section: Resultsmentioning

confidence: 52%

YAMB: metagenome binning using nonlinear dimensionality reduction and density-based clustering

Korzhenkov

2019

Preprint

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“…Subterranean thermal waters of Biragzang possessed substantial number (5.3% in 0.22 µm filter samples) of Firmicutes, belonging to uncultured class D8A-2, which is shown to be enriched in consortium performing methanogenic degradation of volatile fatty acids [ 43 ]. Blast search of Biragzang D8A-2-related ASVs revealed that the most closely related microorganisms were detected in microbial communities of hydrotherms, located at East Tuva Plateau, Russia [ 44 ]. Other major fractions of Firmicutes in Biragzang belonged to Thiobacterales and Ammonifexales orders ( Supplementary Figure S4 ).…”

Section: Resultsmentioning

confidence: 99%

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

Toshchakov

Izotova

Vinogradova

et al. 2021

Biology

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The Greater Caucasus is a part of seismically active Alpine–Himalayan orogenic belt and has been a center of significant volcanic activity during the Quaternary period. That led to the formation of the number of hydrothermal habitats, including subterranean thermal aquifers and surface hot springs. However, there are only a limited number of scientific works reporting on the microbial communities of these habitats. Moreover, all these reports concern only studies of specific microbial taxa, carried out using classical cultivation approaches. In this work, we present first culture-independent study of hydrotherms in the Republic of North Ossetia-Alania, located in the southern part of the North Caucasus. Using 16S metabarcoding, we analyzed the composition of the microbial communities of two subterranean thermal aquifers and terrestrial hot springs of the Karmadon valley. Analysis of correlations between the chemical composition of water and the representation of key taxa allowed us to identify the key factors determining the formation of microbial communities. In addition, we were able to identify a significant number of highly abundant deep phylogenetic lineages. Our study represents a first glance on the thermophilic microbial communities of the North Caucasus and may serve as a basis for further microbiological studies of the extreme habitats of this region.

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Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland

Cited by 8 publications

References 29 publications

Basalt-Hosted Microbial Communities in the Subsurface of the Young Volcanic Island of Surtsey, Iceland

Basalt-Hosted Microbial Communities in the Subsurface of the Young Volcanic Island of Surtsey, Iceland

YAMB: metagenome binning using nonlinear dimensionality reduction and density-based clustering

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

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