Members of the Euwallacea fornicatus species complex exhibit promiscuous mutualism with ambrosia fungi in Taiwan

Carrillo, Joseph Daniel; Rugman‐Jones, Paul F.; Husein, Deena; Stajich, Jason E.; Kasson, Matt T.; Carrillo, Daniel; Stouthamer, Richard; Eskalen, Akif

doi:10.1016/j.fgb.2019.103269

Cited by 27 publications

(29 citation statements)

References 48 publications

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“…DNA sequences of the symbiotic fungi recovered from the Hawaiian beetles also provided little information about potential origin. The F. euwallaceae and G. euwallaceae sequences generated from Hawaiian PSHB have, as yet, never been recovered in the native area of the beetles (Carrillo et al, 2019). However, the Hawaiian fungal sequences were identical to those of the fungi associated with the invasive PSHB populations in California (Eskalen et al, 2012;Lynch et al, 2016) and Israel (Freeman et al, 2013).…”

Section: Discussionmentioning

confidence: 92%

“…The co-occurrence of different cryptic species is not uncommon in this species complex (Stouthamer et al, 2017;Gomez et al, 2018;Smith et al, 2019). For example, in Taiwan, at least three species occur in complete sympatry (Carrillo et al, 2019). Stouthamer et al (2017) previously confirmed the presence of TSHB on the Big Island and on Maui but during the process of publishing that study, a sequence was deposited in GenBank by another group of researchers, indicating that PSHB had been detected on the Big Island.…”

Section: Discussionmentioning

confidence: 96%

“…The remaining abdomen/thorax segments were shipped to the Stouthamer lab (UC Riverside) for molecular identification of the beetle. DNA was extracted from the fungal isolates and sequenced following protocols detailed by Carrillo et al (2019), in which the PCR primers ITS4 and ITS5 (White et al, 1990) were used to amplify the ITS1-5.8S-ITS2 region of the fungal ribosome. Beetles were extracted and sequenced as described above.…”

Section: Identification Of Fungal Species Isolated From Pshb Specimensmentioning

confidence: 99%

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One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands

Rugman‐Jones

Ebrahimi

et al. 2020

PeerJ

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The cryptic species that make up the Euwallacea fornicatus species complex can be readily distinguished via their DNA sequences. Until recently, it was believed that the Hawaiian Islands had been invaded by only one of these cryptic species, E. perbrevis (tea shot hole borer; TSHB). However, following the 2016 deposition of a DNA sequence in the public repository GenBank, it became evident that another species, E. fornicatus (polyphagous shot hole borer; PSHB), had been detected in macadamia orchards on Hawaiʻi Island (the Big Island). We surveyed the two most-populous islands of Hawaiʻi, Big Island and Oʻahu, and herein confirm that populations of TSHB and PSHB are established on both. Beetles were collected using a variety of techniques in macadamia orchards and natural areas. Individual specimens were identified to species using a high-resolution melt assay, described herein and validated by subsequent sequencing of specimens. It remains unclear how long each species has been present in the state, and while neither is currently recognized as causing serious economic or ecological damage in Hawaiʻi, the similarity of the newly-confirmed PSHB population to other damaging invasive PSHB populations around the world is discussed. Although the invasive PSHB populations in Hawaiʻi and California likely have different geographic origins within the beetle’s native range, they share identical Fusarium and Graphium fungal symbionts, neither of which have been isolated from PSHB in that native range.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Identification Of Fungal Species Isolated From Pshb Specimensmentioning

confidence: 99%

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One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands

Rugman‐Jones

Ebrahimi

et al. 2020

PeerJ

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show abstract

Section: Discussionmentioning

confidence: 99%

“…Invasive PSHB populations in California and Hawaiʻi likely have different origins within the beetle's native range, and yet share identical Fusarium and Graphium fungal symbionts, neither of which have been isolated from PSHB anywhere in its native range. Indeed, among invasive populations of the E. fornicatus species complex, only the Fusarium associated with KSHB in California, F. kuroshium, has been found in the native range in Taiwan, although to add further to the conundrum, in Taiwan it has only been isolated from PSHB, and not KSHB (Carrillo et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands (v0.1)"

Smith¹

2020

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The cryptic species that make up the Euwallacea fornicatus species complex can be readily distinguished via their DNA sequences. Until recently, it was believed that the Hawaiian Islands had been invaded by only one of these cryptic species, E. perbrevis (tea shot hole borer; TSHB). However, following the 2016 deposition of a DNA sequence in the public repository GenBank, it became evident that another species, E. fornicatus (polyphagous shot hole borer; PSHB), had been detected in macadamia orchards on Hawaiʻi Island (the Big Island). We surveyed the two most-populous islands of Hawaiʻi, Big Island and Oʻahu, and herein confirm that populations of TSHB and PSHB are established on both. Beetles were collected using a variety of techniques in macadamia orchards and natural areas. Individual specimens were identified to species using a high-resolution melt assay, described herein and validated by subsequent sequencing of specimens. It remains unclear how long each species has been present in the state, and while neither is currently recognized as causing serious economic or ecological damage in Hawaiʻi, the similarity of the newly-confirmed PSHB population to other damaging invasive PSHB populations around the world is discussed. Although the invasive PSHB populations in Hawaiʻi and California likely have different geographic origins within the beetle's native range, they share identical Fusarium and Graphium fungal symbionts, neither of which have been isolated from PSHB in that native range.

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Fungal mutualisms and pathosystems: life and death in the ambrosia beetle mycangia

Joseph

Keyhani

2021

Appl Microbiol Biotechnol

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Members of the Euwallacea fornicatus species complex exhibit promiscuous mutualism with ambrosia fungi in Taiwan

Cited by 27 publications

References 48 publications

One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands

One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands

Peer Review #2 of "One becomes two: second species of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex is established on two Hawaiian Islands (v0.1)"

Fungal mutualisms and pathosystems: life and death in the ambrosia beetle mycangia

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