Fungal mutualisms and pathosystems: life and death in the ambrosia beetle mycangia

Joseph, Ross; Keyhani, Nemat O.

doi:10.1007/s00253-021-11268-0

Cited by 22 publications

(14 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The previous study showed that the mycangia of E. interjectus was located within their oral mycangia as observed by micro-CT scans (Jiang et al, 2019; Figure 1). This fact could provide an explanation for the higher presence of F. kuroshium in the head than the thorax and the abdomen because mycangia act as reservoirs to house the fungi during transport (Joseph and Keyhani, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Ambrosia beetles are wood-inhabiting insects which cultivate fungi as a source of nutrition typically in dead, but occasionally also in healthy, woody hosts (Hulcr and Stelinski, 2017). Most of the ambrosia beetles store and transport their fungal associates to and from their natal galleries in specialized transporting organs called mycangia (singular form: mycangium) (Batra, 1963;Hulcr and Cognato, 2010;Joseph and Keyhani, 2021) or mycetangia (singular form: mycetangium) (Vega and Biedermann, 2020). Once released from the mycangia, the fungi grow in galleries to provide nutrition to the beetles and their offspring (Batra, 1963;Mueller et al, 2005;Bentz and Six, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Many ambrosia beetles and their associated fungi are ecologically constrained to the dying or dead trees and usually remain harmless even after establishment in non-native regions (Cognato et al, 2015). Nevertheless, some invasive symbioses have been found to shift from non-pathogenic saprotrophy in native ranges to prolific tree killing in invaded ranges and cause significant damage (Hulcr and Dunn, 2011;Ranger et al, 2015;Carrillo et al, 2019;Joseph and Keyhani, 2021). Ambrosia beetles are successful invaders (Cognato et al, 2015;Li et al, 2015;Stouthamer et al, 2017;Dzurenko et al, 2021) which exist in part due to their haplodiploid mating system, wide host range, and association with the primary ambrosia fungi, such as Fusarium species, which can act as both the nutritional symbionts and weak phytopathogens (Kasson et al, 2013;O'Donnell et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

2021

View full text Add to dashboard Cite

Ficus carica plantations in Japan were first reported to be infested by an ambrosia beetle species, identified as Euwallacea interjectus, in 1996. The purpose of this study was to determine the symbiotic fungi of female adults of E. interjectus emerging from F. carica trees infected with fig wilt disease (FWD). Dispersal adults (51 females) of E. interjectus, which were collected from logs of an infested fig tree in Hiroshima Prefecture, Western Japan, were separated into three respective body parts (head, thorax, and abdomen) and used for fungal isolation. Isolated fungi were identified based on the morphological characteristics and DNA sequence data. Over 13 species of associated fungi were detected, of which a specific fungus, Fusarium kuroshium, was dominant in female head (including oral mycangia). The plant-pathogenic fungus of FWD, Ceratocystis ficicola, was not observed within any body parts of E. interjectus. We further discussed the relationship among E. interjectus and its associated fungi in fig tree.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

2021

View full text Add to dashboard Cite

show abstract

“…Furthermore, topics in general ecology (e.g., coexisting patterns on patchy resources [21,22]) and applied ecology (e.g., effects of forest management on ecological communities [23,24]) have also been investigated using the insect-basidiocarp system. However, studies on the relationships between insect communities on ascomycete fruit bodies, a sister taxon of basidiomycetes which also produces macroscopic fruit bodies, are quite limited, with examples of symbiotic associations in ambrosia beetles [25], woodwasps [26], and fungus-growing termites [27] and insect pathogens, such as in the genera Beauveria [28], Metarhizium [29], and Ophiocordyceps [30].…”

Section: Introductionmentioning

confidence: 99%

Invertebrate Assemblages on Biscogniauxia Sporocarps on Oak Dead Wood: An Observation Aided by Squirrels

Fukasawa

2021

Forests

View full text Add to dashboard Cite

Dead wood is an important habitat for both fungi and insects, two enormously diverse groups that contribute to forest biodiversity. Unlike the myriad of studies on fungus–insect relationships, insect communities on ascomycete sporocarps are less explored, particularly for those in hidden habitats such as underneath bark. Here, I present my observations of insect community dynamics on Biscogniauxia spp. on oak dead wood from the early anamorphic stage to matured teleomorph stage, aided by the debarking behaviour of squirrels probably targeting on these fungi. In total, 38 insect taxa were observed on Biscogniauxia spp. from March to November. The community composition was significantly correlated with the presence/absence of Biscogniauxia spp. Additionally, Librodor (Glischrochilus) ipsoides, Laemophloeus submonilis, and Neuroctenus castaneus were frequently recorded and closely associated with Biscogniauxia spp. along its change from anamorph to teleomorph. L. submonilis was positively associated with both the anamorph and teleomorph stages. L. ipsoides and N. castaneus were positively associated with only the teleomorph but not with the anamorph stage. N. castaneus reproduced and was found on Biscogniauxia spp. from June to November. These results suggest that sporocarps of Biscogniauxia spp. are important to these insect taxa, depending on their developmental stage.

show abstract

“…Secretory gland cells releasing fatty acids, amino acids, and/or proteins into the mycangium have been proposed to stimulate the growth of ambrosia beetle fungal mutualists and perhaps inhibit antagonistic fungi [13][14][15][16]. Micro-computed tomography is providing an insight into the structure and function of ambrosia beetle mycangia [10,17], but the chemical basis by which mycangia support mutualist growth remains unknown [18].…”

Section: Introductionmentioning

confidence: 99%

Stability of Nuclear and Mitochondrial Reference Genes in Selected Tissues of the Ambrosia Beetle Xylosandrus germanus

Patwa

Ranger

Lehenberger

et al. 2021

Insects

View full text Add to dashboard Cite

The fungus-farming ambrosia beetle Xylosandrus germanus (Blandford) uses a pouch-like structure (i.e., mycangium) to transport spores of its nutritional fungal mutualist. Our current study sought to identify reference genes necessary for future transcriptome analyses aimed at characterizing gene expression within the mycangium. Complementary DNA was synthesized using selected tissue types from laboratory-reared and field-collected X. germanus consisting of the whole body, head + thorax, deflated or inflated mycangium + scutellum, inflated mycangium, and thorax + abdomen. Quantitative reverse-transcription PCR reactions were performed using primers for 28S ribosomal RNA (28S rRNA), arginine kinase (AK), carbamoyl-phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase (CAD), mitochondrial cytochrome oxidase 1 (CO1), and elongation factor-1α (EF1α). Reference gene stability was analyzed using GeNorm, NormFinder, BestKeeper, ΔCt, and a comprehensive final ranking by RefFinder. The gene CO1 was identified as the primary reference gene since it was generally ranked in first or second position among the tissue types containing the mycangium. Reference gene AK was identified as a secondary reference gene. In contrast, EF1α was generally ranked in the last or penultimate place. Identification of two stable reference genes will aid in normalizing the expression of target genes for subsequent gene expression studies of X. germanus’ mycangium.

show abstract

Fungal mutualisms and pathosystems: life and death in the ambrosia beetle mycangia

Cited by 22 publications

References 83 publications

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

Invertebrate Assemblages on Biscogniauxia Sporocarps on Oak Dead Wood: An Observation Aided by Squirrels

Stability of Nuclear and Mitochondrial Reference Genes in Selected Tissues of the Ambrosia Beetle Xylosandrus germanus

Contact Info

Product

Resources

About