Members of a new subgroup of Streptococcus anginosus harbor virulence related genes previously observed in Streptococcus pyogenes

Babbar, Anshu; Kumar, Venkatesan Naveen; Bergmann, René; Barrantes, Israel; Pieper, Dietmar H.; Itzek, Andreas; Nitsche-Schmitz, D. Patric

doi:10.1016/j.ijmm.2017.02.002

Cited by 22 publications

(22 citation statements)

References 42 publications

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“…To many physicians, S. anginosus remains shrouded in medical mystery with confusion regarding its classification hampering detailed investigation and diagnosis of infections 2 . S. pyogenes is well known as ‘group A beta-hemolytic streptococcus’ (GAS) because it demonstrates haemolysis on blood agar and carbohydrate ‘group’ antigens.…”

Section: Discussionmentioning

confidence: 99%

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Acute glomerulonephritis secondary toStreptococcus anginosus

Maharaj¹,

Seegobin

Chrzanowski

et al. 2018

BMJ Case Reports

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is a clinically important pathogen that is emerging globally but remains poorly investigated. Here, we report the first case of acute glomerulonephritis resulting from infection with Glomerulonephritis is typically caused by and reports secondary to other strains including and exist. Infection with in this patient was associated with acute nephritis (haematuria, oedema and hypertension), nephrotic syndrome and progressive azotemia. There was activation of the complement system. The presence of low C1q and elevated anti-C1q binding complexes points to a potential pathogenic role. Testing for streptococcal antigens was strongly positive. Emerging nephritogenic strains of present a significant health concern for both developed and developing countries.

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Section: Discussionmentioning

confidence: 99%

“…Recently, Babbar et al 2 examined α-haemolytic and β-haemolytic S. anginosus isolates from India and Germany. Complementary PCR-based screening for S. pyogenes virulence factors revealed a subgroup of S. anginosus that harbours superantigen and extracellular DNAse coding genes identical to corresponding genes of S. pyogenes .…”

Section: Discussionmentioning

confidence: 99%

Acute glomerulonephritis secondary toStreptococcus anginosus

Maharaj¹,

Seegobin

Chrzanowski

et al. 2018

BMJ Case Reports

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“…The genomes of the 255 SDSE isolates were screened for the presence of 21 S. pyogenes exotoxin encoding genes (speA,speB,speC,speG,speF,speH,speI,speJ,speK,speL,speM,ssa,smeZ,sdaB,sdaD,sdc,spd3,sic,mac,scpA,spyCEP) by a modified multiplex PCR system consisting of five independent reactions as described previously (Babbar et al 2017).…”

Section: Multiplex Pcrmentioning

confidence: 99%

Detection of Streptococcus pyogenes virulence genes in Streptococcus dysgalactiae subsp. equisimilis from Vellore, India

et al. 2018

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Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates were collected from humans infected with SDSE in Vellore, a region in southern India, with high incidence of SDSE infections. Initial evaluation indicated SDSE isolates comprising of 82.35% group G and 17.64% group C. A multiplex PCR system was used to detect 21 gene encoding virulence-associated factors of S. pyogenes, like superantigens, DNases, proteinases, and other immune modulatory toxins. As validated by DNA sequencing of the PCR products, sequences homologous to speC, speG, speH, speI, speL, ssa and smeZ of the family of superantigen coding genes and for DNases like sdaD and sdc were detected in the SDSE collection. Furthermore, there was high abundance (48.12% in group G and 86.6% in group C SDSE) of scpA, the gene coding for C5a peptidase in these isolates. Higher abundance of S. pyogenes virulence factor genes was observed in SDSE of Lancefield group C as compared to group G, even though the incidence rates in former were lower. This study not only substantiates detection of S. pyogenes virulence factor genes in whole genome sequenced SDSE but also makes significant contribution towards the understanding of SDSE and its increasing virulence potential.

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“…these sag genes(Figure 3).To assess the nucleotide sequence of the sagA genes in relation to the phylogeny of the investigated ß-hemolytic S. anginosus strains, multi locus sequence typing (MLST) analysis was performed on all of the ß-hemolytic strains(Bishop et al, 2009) and several of the non-ß-hemolytic strains of our collection. Subsequent analysis was carried out by the Mega7 program and grouped the S. anginosus strains in their recognized subspecies(Babbar et al, 2017;Jensen, Hoshino, & Kilian, 2013), representing a population overview (Figure 4). The ß-hemolytic strains mainly clustered in two of the previously defined subgroups, the S. anginosus subsp.…”

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Heterogeneity of Streptococcus anginosus ß‐hemolysis in relation to CRISPR/Cas

Bauer

Neffgen

Grempels

et al. 2020

Molecular Oral Microbiology

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Streptococcus anginosus is a commensal of the oral mucosa that can cause severe invasive infections. A considerable proportion of Streptococcus anginosus strains are ß‐hemolytic due to the presence of an SLS‐like gene cluster. However, the majority of strains do not display ß‐hemolysis. To investigate ß‐hemolysin heterogeneity in S. anginosus, we determined the presence of sag genes and correlated it with the presence of CRISPR/Cas genes in a collection of ß‐hemolytic and non‐ß‐hemolytic strains. All of the ß‐hemolytic strains carried the sag gene cluster. In contrast to other streptococci, clinical S. anginosus strains that do not display ß‐hemolysis do not harbor sag genes. Phylogenetic analysis of the ß‐hemolytic strains revealed that they belong to two previously defined clusters within S. anginosus. Correlation with CRISPR/Cas genes showed a significant difference for the presence of CRISPR/Cas in ß‐hemolytic versus non‐ß‐hemolytic isolates. The presence of the CRISPR/Cas type IIA or type IIC locus is associated with the absence of sag genes; in 65% of the non‐ß‐hemolytic strains a CRISPR/Cas locus was found, while only 24% of ß‐hemolytic strains carry CRISPR/Cas genes. Further analysis of the spacer content of the CRISPR systems revealed the presence of multiple self‐targeting sequences directed against S. anginosus genes. These results support the hypothesis that horizontal gene transfer is involved in the acquisition of ß‐hemolysin genes and that CRISPR/Cas may limit DNA uptake in S. anginosus.

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Members of a new subgroup of Streptococcus anginosus harbor virulence related genes previously observed in Streptococcus pyogenes

Cited by 22 publications

References 42 publications

Acute glomerulonephritis secondary toStreptococcus anginosus

Acute glomerulonephritis secondary toStreptococcus anginosus

Detection of Streptococcus pyogenes virulence genes in Streptococcus dysgalactiae subsp. equisimilis from Vellore, India

Heterogeneity of Streptococcus anginosus ß‐hemolysis in relation to CRISPR/Cas

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