Melon13-lipoxygenase CmLOX18 may be involved in C6 volatiles biosynthesis in fruit

Chong, Zhang; Cao, Songxiao; Jin, Yongfeng; Ju, Lijun; Chen, Qiang; Xing, Qiaojuan; Qi, Hongyan

doi:10.1038/s41598-017-02559-6

Cited by 23 publications

(19 citation statements)

References 70 publications

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“…In our T 0 silenced lines, a 73–96% reduction in PDC1 expression resulted in a 91–97% decrease in pentanal levels, however such a strong effect was not observed in the T 1 line or the transiently silenced fruit. While significant decreases in pentyl esters following PDC1 silencing strongly indicate that PDC1 contributes to pentanal biosynthesis, the role of LOX enzymes in melon pentanal production should be further investigated, as several LOX genes are expressed in ripe fruit and likely participate in aroma development (Tang et al ., ; Zhang et al ., ).…”

Section: Discussionmentioning

confidence: 97%

PDC1, a pyruvate/α‐ketoacid decarboxylase, is involved in acetaldehyde, propanal and pentanal biosynthesis in melon (Cucumis melo L.) fruit

et al. 2019

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Plant pyruvate decarboxylases (PDC) catalyze the decarboxylation of pyruvate to form acetaldehyde and CO 2 and are well known to play a key role in energy supply via fermentative metabolism in oxygen-limiting conditions. In addition to their role in fermentation, plant PDCs have also been hypothesized to be involved in aroma formation although, to date, there is no direct biochemical evidence for this function. We investigated the role of PDCs in fruit volatile biosynthesis, and identified a melon pyruvate decarboxylase, PDC1, that is highly expressed in ripe fruits. In vitro biochemical characterization of the recombinant PDC1 enzyme showed that it could not only decarboxylate pyruvate, but that it also had significant activity toward other straight-and branched-chain a-ketoacids, greatly expanding the range of substrates previously known to be accepted by the plant enzyme. RNAi-mediated transient and stable silencing of PDC1 expression in melon showed that this gene is involved in acetaldehyde, propanal and pentanal production, while it does not contribute to branched-chain amino acid (BCAA)-derived aldehyde biosynthesis in melon fruit. Importantly, our results not only demonstrate additional functions for the PDC enzyme, but also challenge the long standing hypothesis that PDC is involved in BCAA-derived aldehyde formation in fruit.

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Section: Discussionmentioning

confidence: 97%

PDC1, a pyruvate/α‐ketoacid decarboxylase, is involved in acetaldehyde, propanal and pentanal biosynthesis in melon (Cucumis melo L.) fruit

et al. 2019

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“…To determine the predicted subcellular localization of soybean NRAMP proteins, six GmNRAMP proteins were selected to generate subcellular localization constructs, then transient expression in Arabidopsis protoplasts, which were widely used in subcellular localization analysis of genes not only for soybean, but also for other plant species (Zhang et al, 2016 ; Chong et al, 2017 ; Chu et al, 2017 ; Péron et al, 2017 ; Xu et al, 2017 ). Specifically, the coding region of each GmNRAMP gene was amplified with gene-specific primers shown in Table S3 .…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

et al. 2017

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The NRAMP (natural resistance-associated macrophage protein) family of genes has been widely characterized in organisms ranging from bacteria to yeast, plants, mice, and humans. This gene family plays vital roles in divalent metal ion transport across cellular membranes. As yet, comprehensive analysis of NRAMP family genes has not been reported for soybean. In this study, bioinformatics analysis was conducted to identify 13 soybean NRAMP genes, along with their gene structures, phylogenetic relationships, and transmembrane domains. Expression analysis suggests that GmNRAMP genes function in numerous tissues and development stages. Moreover, soybean NRAMP genes were differentially regulated by deficiencies of N, P, K, Fe, and S, along with toxicities of Fe, Cu, Cd, and Mn. These results indicate that GmNRAMP genes function in many nutrient stress pathways, and might be involved in crosstalk among nutrient stress pathways. Subcellular localization analysis in Arabidopsis protoplasts confirmed the tonoplast or plasma membrane localization of selected soybean NRMAP proteins. Protein-protein interaction analysis found that the networks of three GmNRAMP proteins which putatively interact with nodulin-like proteins, almost distinct from the network that is common to the other 10 soybean NRAMP proteins. Subsequent qRT-PCR results confirmed that these three GmNRMAP genes exhibited enhanced expression in soybean nodules, suggesting potential functions in the transport of Fe or other metal ions in soybean nodules. Overall, the systematic analysis of the GmNRAMP gene family reported herein provides valuable information for further studies on the biological roles of GmNRAMPs in divalent metal ion transport in various soybean tissues under numerous nutrient stresses and soybean-rhizobia symbiosis.

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“…It would be interesting to see if the overexpression of LOX genes produced fruits with a better flavor, although there is no evidence that the activities of LOX isoforms are rate-limiting to volatile synthesis (Garbowicz et al, 2018). Zhang et al (2017a) overexpressed a 13-LOX gene from melon, CmLOX18 , in tomato plants. Fruits from the transgenic lines showed enhanced emission of C6 volatiles together with an increased expression of HPL compared to the wild-type tomato plant.…”

Section: Metabolic Engineering For Fruit Quality Traitsmentioning

confidence: 99%

From Central to Specialized Metabolism: An Overview of Some Secondary Compounds Derived From the Primary Metabolism for Their Role in Conferring Nutritional and Organoleptic Characteristics to Fruit

2019

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Fruit flavor and nutritional characteristics are key quality traits and ones of the main factors influencing consumer preference. Central carbon metabolism, also known as primary metabolism, contributes to the synthesis of intermediate compounds that act as precursors for plant secondary metabolism. Specific and specialized metabolic pathways that evolved from primary metabolism play a key role in the plant’s interaction with its environment. In particular, secondary metabolites present in the fruit serve to increase its attractiveness to seed dispersers and to protect it against biotic and abiotic stresses. As a consequence, several important organoleptic characteristics, such as aroma, color, and fruit nutritional value, rely upon secondary metabolite content. Phenolic and terpenoid compounds are large and diverse classes of secondary metabolites that contribute to fruit quality and have their origin in primary metabolic pathways, while the delicate aroma of ripe fruits is formed by a unique combination of hundreds of volatiles that are derived from primary metabolites. In this review, we show that the manipulation of primary metabolism is a powerful tool to engineer quality traits in fruits, such as the phenolic, terpenoid, and volatile content. The enzymatic reactions responsible for the accumulation of primary precursors are bottlenecks in the transfer of metabolic flux from central to specialized metabolism and should be taken into account to increase the yield of the final products of the biosynthetic pathways. In addition, understanding the connection and regulation of the carbon flow between primary and secondary metabolism is a key factor for the development of fruit cultivars with enhanced organoleptic and nutritional traits.

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Melon13-lipoxygenase CmLOX18 may be involved in C6 volatiles biosynthesis in fruit

Cited by 23 publications

References 70 publications

PDC1, a pyruvate/α‐ketoacid decarboxylase, is involved in acetaldehyde, propanal and pentanal biosynthesis in melon (Cucumis melo L.) fruit

PDC1, a pyruvate/α‐ketoacid decarboxylase, is involved in acetaldehyde, propanal and pentanal biosynthesis in melon (Cucumis melo L.) fruit

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

From Central to Specialized Metabolism: An Overview of Some Secondary Compounds Derived From the Primary Metabolism for Their Role in Conferring Nutritional and Organoleptic Characteristics to Fruit

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