Melittin inhibits lung metastasis of human osteosarcoma: Evidence of wnt/β-catenin signaling pathway participation

Zhu, Haixia; Chen, Deta; Xie, Xiaoxiao; Li, Yumei; Fan, Tao

doi:10.1016/j.toxicon.2021.04.024

Cited by 13 publications

(7 citation statements)

References 55 publications

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“…Several long non-coding RNAs were found to participate in the OS cell progression through the Wnt/β-catenin pathways [9,11,13,14]. The signalling was also revealed to be associated with the OS lung metastasis [12]. These results suggest that β-catenin-dependent Wnt signalling is crucial in the regulation of OS carcinogenesis.…”

Section: Introductionmentioning

confidence: 85%

“…The aberrant expression of Wnt signalling pathway is closely related to various diseases, especially cancers [8]. Growing evidence have been shown that canonical Wnt/β-catenin signalling is involved in the regulation of proliferation, invasion and migration of OS [9][10][11][12]. Several long non-coding RNAs were found to participate in the OS cell progression through the Wnt/β-catenin pathways [9,11,13,14].…”

Section: Introductionmentioning

confidence: 99%

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KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling

Zhang

Wang

et al. 2022

CIM

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Purpose: Osteosarcoma (OS) is the most common malignant solid bone tumor in children and young adults. We aimed to investigate the effects and cellular mechanisms of KMT5A on OS cell activity.Methods: The protein expression was evaluated in the clinical normal, adjacent and OS osteogenic tissues. Knockdown of KMT5A was achieved by KMT5A siRNAs in a human OS cell line, MG63, to detect cell proliferation and metastasis.Results: KMT5A expression was upregulated in clinical OS tissues. Knockdown of KMT5A inhibited cell proliferation but enhanced cell death, with significantly reduced cyclinD1 and Bcl2 and increased cleaved-caspase9 levels. KMT5A knockdown also suppressed OS cell migration and invasion capacity and deceased MMP3 and vimentin expression. β-catenin levels were upregulated in OS tissues and blocking KMT5A resulted in a significant decline in β-catenin expression in the OS cells. Further administration of β-catenin activator remarkably increased protein levels of KMT5A, cyclinD1, Bcl2, MMP3, and vimentin, which showed reversed effects of KMT5A knockdown on OS cell activity.Conclusion: KMT5A knockdown plays an inhibitory role in OS cell proliferation and metastasis through β-catenin signalling, which provides basic evidence and suggests potential targets for OS therapeutic research.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling

Zhang

Wang

et al. 2022

CIM

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“…Besides, Cyclin D, MMP-2, MMP-9, lipoprotein receptor related protein 5 (LRP5), β-catenin and other proteins associated with the Wnt/β-catenin pathway were downregulated after moderate and high concentrations of MEL on human osteosarcoma 143B cells, a malignant bone tumor. The s.c. injection of 143B cells and treatment with MEL in female BALB/c nu/nu nude mice showed a reduction in tumor size, mass and number of lung metastatic nodules, and inhibition of tumor metastatic behavior ( Zhu et al, 2021 ). Glioblastoma multiforme is also a malignant tumor.…”

Section: Anticancer Effectsmentioning

confidence: 99%

Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective

Shi

Xie²,

Yang³

et al. 2022

Front. Pharmacol.

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Bee venom (BV), a type of defensive venom, has been confirmed to have favorable activities, such as anti-tumor, neuroprotective, anti-inflammatory, analgesic, anti-infectivity effects, etc. This study reviewed the recent progress on the pharmacological effects and mechanisms of BV and its main components against cancer, neurological disorders, inflammatory diseases, pain, microbial diseases, liver, kidney, lung and muscle injury, and other diseases in literature during the years 2018–2021. The related target proteins of BV and its main components against the diseases include Akt, mTOR, JNK, Wnt-5α, HIF-1α, NF-κB, JAK2, Nrf2, BDNF, Smad2/3, AMPK, and so on, which are referring to PI3K/Akt/mTOR, MAPK, Wnt/β-catenin, HIF-1α, NF-κB, JAK/STAT, Nrf2/HO-1, TrkB/CREB/BDNF, TGF-β/Smad2/3, and AMPK signaling pathways, etc. Further, with the reported targets, the potential effects and mechanisms on diseases were bioinformatically predicted via Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, disease ontology semantic and enrichment (DOSE) and protein-protein interaction (PPI) analyses. This review provides new insights into the therapeutic effects and mechanisms of BV and its main components on diseases.

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“…Both BV as a whole and its individual components demonstrate anti-cancer activity [ 10 , 13 , 23 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Cancer diseases are the main cause of death in the population, and many of their forms are characterized by high malignancy, metastasis, and resistance to chemotherapy [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Antimicrobial Properties of Honey Bee Venom

Isidorov¹,

Zalewski

Zambrowski

et al. 2023

Molecules

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Due to its great medical and pharmaceutical importance, honey bee venom is considered to be well characterized both chemically and in terms of biomedical activity. However, this study shows that our knowledge of the composition and antimicrobial properties of Apis mellifera venom is incomplete. In this work, the composition of volatile and extractive components of dry and fresh bee venom (BV) was determined by GC-MS, as well as antimicrobial activity against seven types of pathogenic microorganisms. One-hundred and forty-nine organic C1–C19 compounds of different classes were found in the volatile secretions of the studied BV samples. One-hundred and fifty-two organic C2–C36 compounds were registered in ether extracts, and 201 compounds were identified in methanol extracts. More than half of these compounds are new to BV. In microbiological tests involving four species of pathogenic Gram-positive and two species of Gram-negative bacteria, as well as one species of pathogenic fungi, the values of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined for samples of dry BV, as well as ether and methanol extracts from it. Gram-positive bacteria show the greatest sensitivity to the action of all tested drugs. The minimum MIC values for Gram-positive bacteria in the range of 0.12–7.63 ng mL−1 were recorded for whole BV, while for the methanol extract they were 0.49–125 ng mL−1. The ether extracts had a weaker effect on the tested bacteria (MIC values 31.25–500 ng mL−1). Interestingly, Escherichia coli was more sensitive (MIC 7.63–500 ng mL−1) to the action of bee venom compared to Pseudomonas aeruginosa (MIC ≥ 500 ng mL−1). The results of the tests carried out indicate that the antimicrobial effect of BV is associated with the presence of not only peptides, such as melittin, but also low molecular weight metabolites.

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Melittin inhibits lung metastasis of human osteosarcoma: Evidence of wnt/β-catenin signaling pathway participation

Cited by 13 publications

References 55 publications

KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling

KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling

Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective

Chemical Composition and Antimicrobial Properties of Honey Bee Venom

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